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Detection type gene chip for detecting various infectious desease and use thereof

A technology for gene chips, infectious diseases, applied in the fields of resistance to vector-borne diseases, determination/examination of microorganisms, biochemical equipment and methods, etc.

Inactive Publication Date: 2003-10-22
陶开华 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, domestic research on practical microarrays has just started. Up to now, there have been no domestic and foreign applications for these seven important infectious diseases (epidemic hemorrhagic fever, cholera, Escherichia coli 0157:H7, malaria, scrub typhus, and Leptospira). , schistosome) rapid detection gene chip

Method used

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  • Detection type gene chip for detecting various infectious desease and use thereof
  • Detection type gene chip for detecting various infectious desease and use thereof
  • Detection type gene chip for detecting various infectious desease and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] A detection-type gene chip for detecting multiple infectious diseases, including template processing and amplification; carriers such as glass slides, silicon wafers, etc.; modification of arm molecules on the carrier surface; oligonucleotide probes (including detection of oligonucleotides) acid probes and quality control probes) and their curing; detection and analysis, etc. Example 1. Development and preliminary application of a gene chip for detection and typing of malaria parasites

[0058] Malaria is a parasitic disease that seriously endangers human health. Especially in the event of floods, large-scale crowd gatherings, and bioterrorism, the threat of epidemics is still relatively prominent. Efficient, sensitive, and rapid methods of monitoring malaria parasites are the primary prerequisite for malaria control. We established a gene chip for detecting and typing Plasmodium vivax and Plasmodium falciparum, optimized and determined the detection conditions of the ch...

Embodiment 2

[0109] For the detection results of Plasmodium vivax, Plasmodium falciparum infection and mixed infection, see Figure 4 . The results showed that the chip prepared in this paper can specifically detect Plasmodium vivax, Plasmodium falciparum and mixed infection, and no non-specific binding occurred in the control 6 pathogen samples and randomly selected healthy human blood samples, and the difference between the two types of Plasmodium No cross-reactivity. The blood samples of Plasmodium vivax and Plasmodium falciparum of appropriate density were serially diluted with whole blood of healthy people, the DNA template was quickly prepared by Tritonx-100 method, and after PCR parallel amplification, Agarose electrophoresis was performed, and positive bands could be seen under the gel imaging system The maximum dilution of Plasmodium vivax is 10 worms / μl, and that of Plasmodium falciparum is also 10 worms / μl. The above samples were used to prepare templates for chip simulation d...

Embodiment 3

[0122] Example 3. Establishment of a method for detecting Orientia tsutsugamushi DNA by gene chip technology

[0123] Scrub typhus is an insect-borne natural foci disease caused by Orientia tsutsugamushi. In recent years, the epidemic area in my country has been expanding. Because the disease is prone to misdiagnosis, resulting in improper treatment, and even death. Therefore, rapid and accurate diagnosis is extremely important. We selected the 56kD protein gene sequences of three standard strains of Orientia tsutsugamushi, which are both conserved and specific, synthesized oligonucleotide probes, and preliminarily established a diagnostic method for detecting Orientia tsutsugamushi-specific DNA using chip technology

[0124] 1. Design and synthesis of primers and probes

[0125] According to the sequence of the main surface antigen 56kD protein of Orientia tsutsugamushi standard strains Karp Gilliam and Kato[2,3], combined with computer software analysis and NCBI BLAST scre...

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Abstract

The present invention relates to a gene chip for detecting several infections lideases and its application. It can be used for detecting, typing and strain-identifying 7 main infections diseases of epidemic hemorrhagic fever, tsutsugamushi disease, leptospirosis, schistosomiasis, malaria, cholera and hemorrhagic enteritis due to 0157:H7. Said invention can select specific gene probe and PCR primer respectively from S gene of epidemic hemorrhagic fever virus, 56KD protein gene of oriental rickettsia, 23 SRNA gene of leptospirosis, 5D antigen gene of schistosomiasis, SSrRNA gene of malarial parasite, 0157 antigen gene of colibacillus 0157:H7, H7 antigen gene and toxic gene and outer membrane OWP protein gene of cholera vibrio and toxic gene.

Description

technical field [0001] The invention relates to a detection type gene chip for detecting various infectious diseases and the technical field related to the application is medical inspection and gene chip technology. It is a gene chip for the detection of infectious diseases. It detects and types seven major infectious diseases: epidemic hemorrhagic fever, scrub typhus, leptospirosis, schistosomiasis, malaria, cholera, and 0157:H7 hemorrhagic enteritis. Identify strains. Background technique [0002] Epidemic hemorrhagic fever, scrub typhus, leptospirosis, schistosomiasis, malaria, cholera, and hemorrhagic enteritis (Escherichia coli 0157: H7) are still the main infectious diseases that are prone to epidemics in my country and pose a great threat to human health. Additionally, these pathogens are susceptible to biological warfare agents. [0003] Therefore, the rapid detection and determination of the types of these pathogens not only has important clinical value and provide...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCY02A50/30
Inventor 陶开华李越希张锦海
Owner 陶开华
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