Visual measurement method for uric acid based on mobile reaction interface and electrophoretic titration chip

Abstract

The invention discloses a visual measurement method for uric acid based on a mobile reaction interface and an electrophoretic titration chip. The method comprises the following steps of: firstly, injecting a gel mixed with a background buffer solution and sodium acetate into a channel of the electrophoretic titration chip, and filling the background buffer solution in a cathode chamber; then injecting (1) urine to be tested or (2) a mixture of serum and background buffer solution into an anode chamber; adding uricase, and then adding colorless crystal violet and horseradish peroxidase in the anode chamber after the reaction is sufficient, wherein the colorless crystal violet and the horseradish peroxidase react with the reaction product in the original anode chamber to produce colored crystal violet ions; electrophoresing the colored crystal violet ions into the channel and reacting with sodium acetate in the channel to produce a titration neutralization reaction interface; and calculating a linear relationship between a moving distance of a moving reaction interface and the uric acid detecting sample with the known concentration to obtain a contrast curve, thereby calculating theuric acid concentration of the sample to be tested. According to the visual measurement method for uric acid based on the mobile reaction interface and the electrophoretic titration chip, the contentof uric acid in the sample to be tested can be rapidly and effectively determined, the time and sample consumption is low, the portable performance is excellent, and the detection in different environments can be realized.

Description

technical field [0001] The invention relates to a technology in the field of clinical laboratory diagnosis, in particular to a visual measurement method for uric acid based on a mobile reaction interface electrophoretic titration chip. Background technique [0002] The detection of uric acid (UA) in human urine and blood is of great significance to the clinical diagnosis of gout, renal failure, hyperuricemia and other diseases (Baker et al.2005, Am J Med, 118, 816-826; Chen et al. al. 2005, Sens Actuators B Chem, 110, 364-369; Nakanishi et al. 2003, Eur J Epidemiol, 18, 523-530). At present, there are two main methods for the determination of uric acid in urine and blood, namely, enzymatic method and non-enzymatic method. In the enzyme-based method, uric acid (UA) is decomposed by uricase oxidase (UOD) to produce hydrogen peroxide, carbon dioxide and allantoin, and then hydrogen peroxide is measured by quantum dot / dual enzyme fluorescence method to obtain UA content (Azmi ...

AI Technical Summary

Problems solved by technology

Although these POCT devices are portable in the detection of UA, they still suffer from high cost and poor stability due to chemical and environmental interference.

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