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Paper chip enzyme-linked immunoassay test card for combined multi-tumor marker test

A tumor marker and enzyme-linked immunosorbent technology, which is applied in the field of paper chip enzyme-linked immunosorbent test cards, can solve the problems of inconvenient clinical application of tumor diagnosis, and achieve the effects of convenient on-site detection and application, high sensitivity and improved repeatability.

Inactive Publication Date: 2014-04-23
INST OF ELECTRONICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with the traditional enzyme-linked immunoassay method, these paper chips have significantly improved the detection cost and analysis time, but they still need to be equipped with corresponding kits when used, which brings inconvenience to the clinical field application of tumor diagnosis

Method used

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  • Paper chip enzyme-linked immunoassay test card for combined multi-tumor marker test
  • Paper chip enzyme-linked immunoassay test card for combined multi-tumor marker test
  • Paper chip enzyme-linked immunoassay test card for combined multi-tumor marker test

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1: Detection of three tumor markers of AFP, CEA and AFU

[0050] AFP: It is an embryonic protein synthesized by the liver during the fetal period, and it is the most valuable tumor marker for detecting primary liver cancer. Under normal circumstances, it only exists in the blood of the fetus. Normal adult liver cells do not produce AFP, but when the liver cells become malignant, they can regain the ability to synthesize AFP. And there is a certain correlation between AFP content and tumor size and pathological type.

[0051] CEA: an acidic glycoprotein, which exists on the cell surface of the embryonic gastrointestinal mucosal epithelium and some malignant tissues, and is an important general tumor marker. The content of CEA in the serum of normal adults is extremely low, but it can be re-expressed when a tumor occurs, and its content has a certain relationship with the size of the tumor and whether there is metastasis. , the increase of CEA was particularly o...

Embodiment 2

[0058] Example 2: Detection of four tumor markers CEA, NSE, CYFRA21-1 and CA125

[0059] NSE: Neuron-specific enolase is an acidic protease that participates in glycolysis, and its main function is to catalyze the conversion of 2-phosphate-glycerol into enol phosphopyruvate. The glycolysis of cancer tissue is enhanced, the cell proliferation cycle is accelerated, and the release of NSE into the blood increases, resulting in an increase in the level of this enzyme in the serum. It is a tumor marker for small cell lung cancer (SCLC) and neuroblastoma. Using NSE to increase to monitor SCLC recurrence is 4-12 weeks earlier than clinically determined recurrence.

[0060] CYFRA21-1: A fragment of cytokeratin 19, mainly present in the cytoplasm of tumor cells of epithelial origin such as lung cancer and esophageal cancer. It can be released into the blood when tumor cells are lysed or necrotic, and the positive rate of CYFRA21-1 in lung cancer patients can be as high as 60% to 85%. ...

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Abstract

The invention discloses a paper chip enzyme-linked immunoassay test card for a combined multi-tumor marker test. The test card is characterized in that upper, middle and lower filter paper chips, a double-sided tape and a paper-based slide frame are arranged between an upper shell and a lower shell; the upper, middle and lower filter paper chips refer to wax patterned filter paper chips which are provided with hydrophilic and hydrophobic regions and are alternately arranged respectively; a sample introduction and liquid feeding window is formed in an upper shell body; the upper filter paper chip is arranged between the upper shell and the double-sided tape; the middle filter paper chip is arranged between the double-sided tape and the lower filter paper chip, and sensitive regions which are distributed in arrays and used for fixing different reaction reagents are arranged on the middle filter paper chip; the water-based slide frame is arranged between the lower filter paper chip and the lower shell; the upper and middle filter paper chips are bonded through the double-sided tape; the lower filter paper chip slides on the water-based slide frame relative to the upper and middle filter paper chips, so that the different reaction reagents can sequentially flow among the multiple layers of filter paper chips. According to the test card, an early diagnosis device applied in clinical site is provided for patients.

Description

technical field [0001] The invention relates to the technical field of immunoassay, in particular to a paper chip enzyme-linked immunoassay card which can be used for combined detection of multiple tumor markers. Background technique [0002] Tumor marker detection is a new method of tumor diagnosis developed in recent years. It has a unique role in early diagnosis, especially in the detection of micrometastasis. It can complement each other's advantages and disadvantages and improve the positive detection rate. At present, the quantitative detection of various tumor markers mainly relies on the traditional enzyme-linked immunosorbent assay (ELISA) and chemiluminescent enzyme-linked immunosorbent assay (CLIA). The processing process consumes a large amount of expensive antibody reagents, and the analysis time is long and the operation is complicated, which is not conducive to the rapid detection application of multiple indicators in the clinical field. [0003] Microfluidi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574
CPCG01N33/57484G01N33/54386
Inventor 罗金平蔡新霞刘军涛石文韬王蜜霞
Owner INST OF ELECTRONICS CHINESE ACAD OF SCI
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