24 results about "Tumor marker test" patented technology

The application provides an AFP detection kit and application thereof, relates to the technical field of tumor marker detection, and the kit is a double-antibody sandwich ELISA kit. The kit contains two AFP monoclonal antibodies, AFPH8 and AFPE2. The amino acid sequences of the CDR of the heavy chain of AFPH8 are shown in SEQ ID NO. 4-6, the amino acid sequences of the CDR of the light chain of AFPH8 are shown in SEQ ID NO. 7-9, the amino acid sequences of the CDR of the heavy chain of AFPE2 are shown in SEQ ID NO. 12-14, and the amino acid sequences of the CDR of the light chain of AFPE2 are shown in SEQ ID NO. 15-17. Experiments show that the kit has high sensitivity and can detect slightly elevated AFP in initial serum.

The invention relates to the technical field of tumor marker detection, in particular to a breast cancer lung metastasis related biomarker screening system which comprises a differential genetic factor mining module, a function association module, a verification screening module, an interference elimination module and an optimization integration module. According to the method, the candidate genetic factors highly associated with lung metastasis are extracted, and the expression fluctuation characteristics are combined to carry out difference analysis, so that the preliminary screening accuracy is improved, pathological information and pathway annotation are integrated to construct an action network, pathway weights are analyzed, and functional expressions of the candidate factors are quantitatively evaluated; an independent sample is called to verify transcription consistency and clinical relevance, interference factors with poor repeatability are eliminated, non-tumor tissue high-expression interference items are rejected in combination with background transcription characteristics, the specificity and adaptability of screening results are improved, and finally a marker set with metastasis mechanism indicating significance and prognosis prediction value is formed through comprehensive evaluation. And clinical transformation support is provided for identification of lung metastasis of breast cancer.

[Problem] In fields such as tumor marker test and pregnancy test kit, it is known to concentrate a target component (protein) by utilizing a superabsorbent polymer (SAP) as a method for improving test sensitivity. However, due to the powder form, SAP exhibits difficulties in handleability and formability and also causes problems such as falling off. Furthermore, the concentration effect is hardly at a sufficient level. The present invention has been made in view of such problems and on the basis of the discovery that a fiber containing an ammonium salt-type carboxyl group and a crosslinked structure serves as a material for protein concentration having excellent handleability and formability, in addition to having a high protein concentration effect. [Solution] A fiber for protein concentration is a fiber that contains an ammonium salt-type carboxyl group and a crosslinked structure, and is characterized by having a water absorption ratio of 4-300 times.

The invention belongs to the technical field of micro-fluidic chips, and discloses a Tesla valve micro-fluidic chip for tumor marker detection. The chip comprises an upper flow channel layer, a lower flow channel layer and a base which are stacked, wherein the upper flow channel layer is provided with a driving structure; the driving structure comprises a first driver and a second driver which are respectively used for controlling a cleaning solution and a detection reagent; a fluid channel integrated with a Tesla valve is arranged in the upper runner layer and is used for preventing reagent backflow and cross contamination; the lower runner layer is provided with a detection interface; the base is provided with a slidable mechanical cleaning module used for replacing manual plate cleaning operation. Through the unique structural design, multiple steps of operation such as sample adding, incubation, cleaning and color developing required by traditional ELISA are integrated in a single chip and are completed through manual driving, the detection performance is maintained, meanwhile, the operation complexity, the detection time, reagent consumption and dependence on large equipment are remarkably reduced, and the detection efficiency is improved. And the method is suitable for on-site rapid detection (POCT) of various tumor markers.

The application relates to the technical field of tumor marker detection, in particular to a sensor for detecting a tumor marker EpCAM as well as a preparation method and application thereof. The sensor is obtained by self-assembly of a compound of formula 1) and a compound of formula 2) into diacetylene liposomes, then a fluorescent dye-labeled EpCAM aptamer is combined with the diacetylene liposomes through electrostatic interaction to obtain the sensor; the sensor can be used as an activatable fluorescent sensor to sensitively and selectively detect EpCAM and has the effect of fast response, and can realize rapid and sensitive analysis and detection of EpCAM at the water solution level, the living cell level and the animal level; formula 1); formula 2).

The invention belongs to the technical field of biological medicine, and particularly discloses a combined tumor marker for noninvasive detection of oral cancer and application of the combined tumor marker. The invention provides application of a reagent for detecting a biomarker in preparation of a product for diagnosing or predicting oral cancer. The biomarker is an RBMX gene and / or an NEK2 gene. The invention finds that the two oral cancer carcinogenic key molecules are significantly highly expressed in oral cancer tissues, and the activity, migration and invasion ability of oral cancer cells can be inhibited by knocking down the expression of RBMX or NEK2, and the cis-platinum drug resistance of the oral cancer cells can be inhibited. According to the invention, RBMX or NEK2 is taken as a tumor marker to detect the oral cancer and has good diagnostic efficacy, and the RBMX and the NEK2 are combined for use, so that the sensitivity and the specificity of diagnosis can be further improved, and the risk of the oral cancer can be more comprehensively judged. The invention also provides application of the two pathogenic factors as inhibition targets in preparation of drugs for oral cancer.

The invention discloses a medical kit for detecting tumor markers. The medical kit comprises a kit body; the blocking structure comprises flange plates and a buffer pad, the flange plates are formed in the middles of the side walls of the two opposite sides of the box body, and the buffer pad is connected between the two flange plates; the damping cushion block is embedded in the lower empty bin and placed on the bottom wall, the damping cushion block comprises a plurality of unit plate blocks, each unit plate block comprises a sponge block and a base plate, the sponge block is bonded to the upper portion of the base plate, a plurality of inserting holes are formed in the sponge block, a plurality of magnetic plates are connected to the outer edge of the base plate in a turnover mode, and the magnetic plates are attached to the side portions of the sponge blocks; every two adjacent magnetic plates are connected together through a film strip, rubber strips are laid on the outer sides of the magnetic plates and covered with isolating films, and the magnetic plates of every two adjacent unit plates are magnetically attracted together. According to the kit, the problem that a film sealing plate, a microporous elisa plate and a plurality of liquid bottles of an existing kit for detecting the tumor markers are easily damaged due to collision is solved.

The utility model discloses a kind of kits for multiple tumor marker detection, it is related to medical instrument technical field, including box and sample liquid preparation component, the box one end is equipped with receiving groove, the sample liquid preparation component is set in receiving groove inside, the sample liquid preparation component includes the needle cylinder being set to outside, needle cylinder inside is filled with blood sample diluent in advance.This application provides a kind of kits for multiple tumor marker detection, the blood sample of examinee is injected by needle cylinder one side middle part injection port using syringe, and mixed with the blood sample diluent filled in advance in needle cylinder, operator handheld needle cylinder and through the cooperation of rotating shaft sleeve and receiving groove side wall connecting port, swing needle cylinder so that blood sample and diluent are mixed uniformly, squeeze needle handle again, and sample liquid flows to detection area through distribution flow channel, the sample liquid preparation component is integrated in the side of box in this application, it is convenient for user to prepare blood sample quickly, and it is more practical.

The application discloses an engineering bacterium, a biosensor and a detection method thereof. The biosensor takes the modified Acinetobacter baylyi ADP1 as a carrier, utilizes the natural nucleic acid uptake capacity thereof, and can autonomously absorb exogenous nucleic acid without external induction; after the nucleic acid is internalized, the hairpin structure restriction of the blocked gRNA is removed, the CRISPRa system is activated, the dCas9-SoxS protein is targeted and combined with the target promoter, the CymA-MtrCAB gene expression is started, and the specific recognition of the nucleic acid sequence is realized. The system integrates the CymA-MtrCAB transmembrane electron conduction module, converts the biological recognition signal into an electrochemical signal output in real time through the membrane protein-mediated electron transfer chain, and establishes a detection path of 'nucleic acid recognition-gene activation-electric response'. The biosensor has the advantages of modular structure, high response specificity, high detection sensitivity, wide environmental adaptability and the like, is suitable for tumor marker detection, wearable health monitoring and other biological molecule recognition scenes, and provides a novel programmable biological perception solution with high universality.

This invention discloses a tumor marker detection system based on an antibody-modified gold nanoparticle periodic linear array, belonging to the field of biomolecular detection. In this invention, a primary antibody is modified onto gold nanowires, and a nanolabel of antibody molecules modified onto gold nanoparticles is prepared as a detection tag (secondary antibody) for tumor markers. A test solution mixed with the secondary antibody is drop-coated onto the structure, and after a sufficient reaction time, the entire structure is rinsed. When light is incident perpendicularly on the gold nanowire array, surface plasmon resonance (SPR) occurs. The tumor markers (antigens) present in the solution are specifically adsorbed with the primary and secondary antibodies, forming a double-antibody sandwich structure, which changes the macroscopic color of the structure. By observing whether the color changes in different regions of the nanowire array after detection, the presence of tumor markers in the test solution can be determined. This invention's system can simultaneously detect multiple tumor markers and features short detection time, small size, low cost, high sensitivity, and ease of outdoor observation.

The invention belongs to the technical field of biological nanotechnology, and particularly relates to a DNA triangular prism support structure and a DNA enzyme walker guiding directional movement based on a DNA triangular prism support track. The six vertexes of the constructed three-dimensional DNA triangular prism scaffold structure can be used for designing specific cohesive ends and fixing at least three different substrate chains and a DNAzyme walker, and the structural characteristics enable DNAzyme to perform sequential cutting according to the principle of the shortest spatial distance between substrates under the assistance of Mg, so that the stability of the scaffold structure is improved. According to the system, the derailing phenomenon in a traditional free system is completely avoided through the physical confinement effect, and the cutting path can be dynamically adjusted when an adjacent substrate is removed. The technical scheme has the advantages of low preparation cost, strong structure programmability, high cutting specificity, controllable operation direction and the like, and can be widely applied to the biomedical fields of tumor marker detection, targeted drug delivery, cancer precise treatment and the like.

This invention discloses an electrochemiluminescence microfluidic chip for the detection of multiple tumor markers, relating to the field of biodetection technology. It includes a substrate and a microfluidic channel system, an electrochemiluminescence detection unit, and a signal acquisition interface integrated on the substrate. The microfluidic channel system includes a sample inlet, a shunt structure, multiple parallel and equally spaced detection channels, and a waste liquid pool connected in sequence. The shunt structure includes a main channel and multiple branch channels integrally formed with the main channel. In this invention, the microfluidic channel system, the electrochemiluminescence detection unit, and the signal acquisition interface are integrated on the same substrate, achieving an integrated design for sample transmission, reaction, detection, and signal export. Compared to traditional distributed detection technologies, this significantly simplifies the detection operation steps, reduces the professional skills required of operators, and shortens the detection cycle, meeting the needs of rapid clinical diagnosis.

The invention discloses a tumor marker detection method and system based on DNA quantitative analysis in the technical field of cell images. The detection method comprises the following steps: S1, staining a tissue / cell section; s2, scanning the stained tissue / cell section to obtain a digital section image; s3, performing cell segmentation on the slice image to obtain a segmented image containing a cell mask region, and performing DNA quantitative analysis and cytological analysis and classification on the segmented image; s4, performing virtual dyeing on the slice image; and S5, acquiring a DNA quantitative analysis result and a cytological analysis and classification result, and jointly outputting the results in combination with the virtual dyeing image. According to the detection method, a complete solution with high objectivity and high clinical practicability is jointly formed through collaborative diagnosis of DNA quantitative analysis and cytological analysis and by combining virtual staining, the collaborative diagnosis efficiency is generated, and the problem that practical application is difficult due to the fact that current pathological AI diagnosis is insufficient in accuracy is solved.

The invention discloses a marker group for screening and diagnosing ovarian cancer and application of the marker group, and belongs to the technical field of molecular biology. The marker group comprises at least four of phospholipids and polypeptides as shown in sequences SEQ ID NO. 1-41. When being used for auxiliary diagnosis and early screening of ovarian cancer, the marker combination constructed by the invention has more excellent sensitivity and specificity, and the auxiliary diagnosis results of the marker combination are partially or completely superior to those of an ROMA index ovarian cancer risk prediction model recommended by a current clinical guide. The early screening result is also partially or completely superior to that of tumor marker CA125 detection and HE4 detection with the highest ovarian cancer early screening value at present. The kit is expected to be applied to diagnosis and treatment of ovarian cancer.

The application belongs to the technical field of biological detection, and particularly relates to a magnetic bead and a method for detecting glycosylation tumor marker. The magnetic bead for detecting the glycosylation tumor marker is a boronic acid-based magnetic bead, and the magnetic microspheres on the surface of the magnetic bead contain boronic acid groups, the size of the magnetic microspheres is 300-500 nm, and the content of boron elements on the surface of the magnetic microspheres is greater than or equal to 1000 mg / g. Specifically, the boronic acid-based magnetic bead is used as a solid-phase extraction agent, and the method comprises the following steps: the combination of the magnetic bead and the glycosylation tumor marker, the blocking of the magnetic bead, and the coupling of the corresponding antibody of a signal molecule label, and the detection of the magnetic bead on a corresponding signal molecule detection instrument after magnetic separation. The application has the advantages of simple route, rapid and convenient operation, low cost and the like for detecting the glycosylation tumor marker.

The application discloses a marker group for ovarian cancer screening and diagnosis and application thereof, and belongs to the field of molecular biological technology.The marker group comprises at least four of phospholipids and polypeptides shown in sequences SEQ ID NO.1-41.The marker group constructed by the application has more excellent sensitivity and specificity when used for the auxiliary diagnosis and early screening of ovarian cancer, and the auxiliary diagnosis results are all partially or completely superior to the ROMA index ovarian cancer risk prediction model recommended by the current clinical guideline.The early screening result is also partially or completely superior to the detection of the tumor marker CA125 and the detection of HE4, which are the most valuable tumor markers for the early screening of ovarian cancer, and the application is expected to be applied to the diagnosis and treatment of ovarian cancer.

The application belongs to the technical field of electrochemiluminescence immunosensor, discloses a kind of electrochemiluminescence immunosensor for detecting tumor marker and application thereof, and the preparation system of electrochemiluminescence immunosensor for detecting tumor marker includes: graphite carbon nitride preparation module, working electrode pretreatment module, central control module, antigen standard solution preparation module, modified electrode preparation module, immunosensor preparation module, tumor marker detection module, sensor detection curve drawing module, data storage module, update display module.The electrochemiluminescence immunosensor for detecting tumor marker provided by the application has high analysis sensitivity, strong specificity, is simple to use and low in cost.The application improves the sensitivity of electrochemiluminescence immunosensor by dropping coating bovine serum albumin solution and tumor marker antigen standard solution on the surface of graphite carbon nitride working electrode, and greatly simplifies the electrode preparation process without enzyme and label.

The invention belongs to the field of tumor marker detection and application, and particularly relates to an electrochemiluminescence detection kit based on a lanthanide complex and a preparation method, the lanthanide complex Tb-dCOP constructed according to a specific molar ratio is used as a core electrochemiluminescence material, the lanthanide complex Tb-dCOP has relatively high conductivity and stability, can generate an extremely strong ECL signal, and can be used for detecting tumor markers. According to the kit, AgNPs (at) ZnONFs are used as a co-reaction reagent and a signal stabilizer and are combined with a DNA chain fixing carrier, an efficient sensing interface is constructed, ultra-sensitive detection of microRNA-21 (miRNA-21) is achieved through a dendritic double-chain hybridization chain reaction (D-HCR), and the detection limit reaches 46.8 amol / L. Compared with the prior art, the kit has the advantages that the sensitivity is high, and the sensitivity is high. The invention effectively overcomes the problems of low sensitivity and poor accuracy in detection of tumor markers such as miRNA-21 and the like in the prior art, and has the advantages of lower detection limit, better stability, wider linear range and the like.

The embodiment of the invention provides a tumor marker detection method and related equipment, and belongs to the technical field of medical detection. The method comprises the following steps: modifying corresponding antibodies on a preset sensing chip according to a plurality of different tumor marker antibodies to construct a preset micro-fluidic chip; wherein the preset micro-fluidic chip comprises a micro-fluidic channel, and the micro-fluidic channel comprises a reference region and a sensing structure corresponding to each tumor marker antibody; inputting a to-be-detected blood sample into the micro-fluidic channel, and dynamically collecting to obtain chip detection video data; performing image analysis according to the chip detection video data to obtain preset gray scale change data, and further calculating the preset gray scale change data to obtain marker concentration data; wherein the preset gray scale change data comprises the gray scale change data corresponding to the reference area and each sensing structure. According to the embodiment of the invention, high-sensitivity quantitative detection can be realized, and the accuracy and efficiency of diagnosis are effectively improved.

The invention provides a kit for detecting a tumor marker Legumain. The kit comprises a biosensor and a Legumain antibody solution, wherein the biosensor is combined with an antibody Anti-Legumian-Ab1, and the Legumain antibody solution is marked by colloidal gold. The invention also provides a method for detecting the tumor marker Legumain by using the kit, and the method comprises the following steps: exposing the biosensor combined with the antibody Anti-Legumian-Ab1 to a human serum sample, transferring the biosensor combined with the target molecule to a colloidal gold labeled Legumain antibody solution, and detecting the tumor marker Legumain by using the kit. Determining the amount of an Anti-Legumain-Ab2 antibody labeled by colloidal gold deposited on the biosensor by using a biological membrane interference method; and according to the deposition amount of the Anti-Legumain-Ab2 antibody labeled by colloidal gold, carrying out quantitative analysis on the antibody. The method has high detection sensitivity.

The application relates to the technical field of tumor marker detection, in particular to a tumor sampling and tumor marker detection system. The system comprises: collecting concentration values; after classifying patients, clustering the patients based on the concentration values, and determining the abnormalities of each tumor marker; then analyzing the progress values of all the detected tumor markers; then analyzing the fluctuation conditions based on the progress values, calculating a risk assessment index according to the proportion of fluctuation conditions of the patients in the same category and a fluctuation index; determining the interval length of the next time through the risk assessment index; and re-performing tumor marker detection after the adjusted interval length. The application solves the problem of time misjudgment caused by false progress.

The application belongs to the technical field of marker detection, and discloses a multi-channel Raman spectrum analysis system for tumor marker detection, which comprises a pretreatment module, an acquisition module, an enhancement processing module, an analysis module and an output module; the pretreatment module is used for uniformly coating electromagnetic nanomaterial on the surface of a target tissue section; the acquisition module is used for acquiring the Raman spectrum of the target tissue section, and the Raman spectrum comprises a plurality of Raman scattering lights with different wavelengths; the enhancement processing module is used for converting the Raman spectrum acquired by the acquisition module into an electric signal, and also used for enhancing the electric signal; the analysis module is used for identifying tumor markers by analyzing the enhanced electric signal; and the output module is used for marking tumor markers according to the identification result of the analysis module, and also used for outputting a target tissue pathological image with the tumor markers marked.

The utility model belongs to the technical field of marker detection, and particularly relates to a tumor marker detection device based on a sers spectrum technology, which comprises a treatment bin, a partition plate is arranged on the inner side of the treatment bin and divides the inside of the treatment bin into an adsorption bin and a cleaning bin, an adsorption structure is arranged in the adsorption bin, and a second drainage pipe is arranged at the bottom of the adsorption bin. A central column is fixed to the position, located at the center of the partition plate, in the treatment bin, a driving structure used for driving the adsorption structure to move into the cleaning bin from the interior of the adsorption bin is installed at the top of the central column, a detection bin is installed at the bottom of the cleaning bin, and a first drainage pipe is installed at the bottom of the detection bin; a detection assembly for detecting tumor markers is mounted in the detection bin, the adsorption structure comprises an adsorption plate, and first electromagnets are uniformly arranged in the adsorption plate. According to the utility model, the target object can be accurately separated, useless body fluid can be discharged, impurity interference can be obviously reduced through cleaning, and the detection accuracy can be greatly improved.

The invention relates to the technical field of biomedicine, in particular to a methylation marker for diagnosis of ovarian clear cell carcinoma. By detecting the methylation levels of the methylation markers MIR148A, CRLF1 and FER1L4 in an ovarian clear cell carcinoma related sample, specific diagnosis of the type of ovarian cancer can be realized at the molecular level. Compared with traditional histopathologic diagnosis or conventional tumor marker detection (such as CA125, HE4 and the like), the detection system provided by the invention can realize high-sensitivity, high-specificity and low-invasiveness diagnosis in tissue or body fluid samples, and the accuracy of early detection and typing diagnosis is effectively improved.