39 results about "Tumor marker test" patented technology

The application provides an AFP detection kit and application thereof, relates to the technical field of tumor marker detection, and the kit is a double-antibody sandwich ELISA kit. The kit contains two AFP monoclonal antibodies, AFPH8 and AFPE2. The amino acid sequences of the CDR of the heavy chain of AFPH8 are shown in SEQ ID NO. 4-6, the amino acid sequences of the CDR of the light chain of AFPH8 are shown in SEQ ID NO. 7-9, the amino acid sequences of the CDR of the heavy chain of AFPE2 are shown in SEQ ID NO. 12-14, and the amino acid sequences of the CDR of the light chain of AFPE2 are shown in SEQ ID NO. 15-17. Experiments show that the kit has high sensitivity and can detect slightly elevated AFP in initial serum.

The invention relates to the technical field of tumor marker detection, in particular to a breast cancer lung metastasis related biomarker screening system which comprises a differential genetic factor mining module, a function association module, a verification screening module, an interference elimination module and an optimization integration module. According to the method, the candidate genetic factors highly associated with lung metastasis are extracted, and the expression fluctuation characteristics are combined to carry out difference analysis, so that the preliminary screening accuracy is improved, pathological information and pathway annotation are integrated to construct an action network, pathway weights are analyzed, and functional expressions of the candidate factors are quantitatively evaluated; an independent sample is called to verify transcription consistency and clinical relevance, interference factors with poor repeatability are eliminated, non-tumor tissue high-expression interference items are rejected in combination with background transcription characteristics, the specificity and adaptability of screening results are improved, and finally a marker set with metastasis mechanism indicating significance and prognosis prediction value is formed through comprehensive evaluation. And clinical transformation support is provided for identification of lung metastasis of breast cancer.

[Problem] In fields such as tumor marker test and pregnancy test kit, it is known to concentrate a target component (protein) by utilizing a superabsorbent polymer (SAP) as a method for improving test sensitivity. However, due to the powder form, SAP exhibits difficulties in handleability and formability and also causes problems such as falling off. Furthermore, the concentration effect is hardly at a sufficient level. The present invention has been made in view of such problems and on the basis of the discovery that a fiber containing an ammonium salt-type carboxyl group and a crosslinked structure serves as a material for protein concentration having excellent handleability and formability, in addition to having a high protein concentration effect. [Solution] A fiber for protein concentration is a fiber that contains an ammonium salt-type carboxyl group and a crosslinked structure, and is characterized by having a water absorption ratio of 4-300 times.

The invention belongs to the technical field of micro-fluidic chips, and discloses a Tesla valve micro-fluidic chip for tumor marker detection. The chip comprises an upper flow channel layer, a lower flow channel layer and a base which are stacked, wherein the upper flow channel layer is provided with a driving structure; the driving structure comprises a first driver and a second driver which are respectively used for controlling a cleaning solution and a detection reagent; a fluid channel integrated with a Tesla valve is arranged in the upper runner layer and is used for preventing reagent backflow and cross contamination; the lower runner layer is provided with a detection interface; the base is provided with a slidable mechanical cleaning module used for replacing manual plate cleaning operation. Through the unique structural design, multiple steps of operation such as sample adding, incubation, cleaning and color developing required by traditional ELISA are integrated in a single chip and are completed through manual driving, the detection performance is maintained, meanwhile, the operation complexity, the detection time, reagent consumption and dependence on large equipment are remarkably reduced, and the detection efficiency is improved. And the method is suitable for on-site rapid detection (POCT) of various tumor markers.

The invention discloses engineering bacteria, a biosensor and a detection method thereof. According to the sensor, modified Acinetobacter bayli ADP1 is taken as a carrier, and exogenous nucleic acid can be autonomously absorbed without external induction by utilizing the natural nucleic acid uptake capability of the carrier; after nucleic acid internalization, hairpin structure limitation of the blocking type gRNA is relieved, a CRISPRa system is activated, dCas9-SoxS protein is driven to be combined with a target promoter in a targeted mode, CymA-MtrCAB gene expression is started, and specific recognition of a nucleic acid sequence is achieved. The system is integrated with a CymA-MtrCAB transmembrane electron conduction module, a biological recognition signal is converted into an electrochemical signal in real time to be output through a membrane protein mediated electron transfer chain, and a nucleic acid recognition-gene activation-electric response detection path is established. The biosensor has the advantages of structural modularization, high response specificity, high detection sensitivity, wide environment adaptability and the like, is suitable for tumor marker detection, wearable health monitoring and other biomolecule recognition scenes, and provides a novel biosensing solution which is high in universality and programmable.

The invention provides a compound shown as a formula (I), and the compound can be used for recognizing a prostate specific antigen, can efficiently detect prostate cancer, and has a relatively high application value. # imgabs0 #

The application relates to the technical field of tumor marker detection, in particular to a sensor for detecting a tumor marker EpCAM as well as a preparation method and application thereof. The sensor is obtained by self-assembly of a compound of formula 1) and a compound of formula 2) into diacetylene liposomes, then a fluorescent dye-labeled EpCAM aptamer is combined with the diacetylene liposomes through electrostatic interaction to obtain the sensor; the sensor can be used as an activatable fluorescent sensor to sensitively and selectively detect EpCAM and has the effect of fast response, and can realize rapid and sensitive analysis and detection of EpCAM at the water solution level, the living cell level and the animal level; formula 1); formula 2).

The invention belongs to the technical field of biological medicine, and particularly discloses a combined tumor marker for noninvasive detection of oral cancer and application of the combined tumor marker. The invention provides application of a reagent for detecting a biomarker in preparation of a product for diagnosing or predicting oral cancer. The biomarker is an RBMX gene and / or an NEK2 gene. The invention finds that the two oral cancer carcinogenic key molecules are significantly highly expressed in oral cancer tissues, and the activity, migration and invasion ability of oral cancer cells can be inhibited by knocking down the expression of RBMX or NEK2, and the cis-platinum drug resistance of the oral cancer cells can be inhibited. According to the invention, RBMX or NEK2 is taken as a tumor marker to detect the oral cancer and has good diagnostic efficacy, and the RBMX and the NEK2 are combined for use, so that the sensitivity and the specificity of diagnosis can be further improved, and the risk of the oral cancer can be more comprehensively judged. The invention also provides application of the two pathogenic factors as inhibition targets in preparation of drugs for oral cancer.

The invention discloses a medical kit for detecting tumor markers. The medical kit comprises a kit body; the blocking structure comprises flange plates and a buffer pad, the flange plates are formed in the middles of the side walls of the two opposite sides of the box body, and the buffer pad is connected between the two flange plates; the damping cushion block is embedded in the lower empty bin and placed on the bottom wall, the damping cushion block comprises a plurality of unit plate blocks, each unit plate block comprises a sponge block and a base plate, the sponge block is bonded to the upper portion of the base plate, a plurality of inserting holes are formed in the sponge block, a plurality of magnetic plates are connected to the outer edge of the base plate in a turnover mode, and the magnetic plates are attached to the side portions of the sponge blocks; every two adjacent magnetic plates are connected together through a film strip, rubber strips are laid on the outer sides of the magnetic plates and covered with isolating films, and the magnetic plates of every two adjacent unit plates are magnetically attracted together. According to the kit, the problem that a film sealing plate, a microporous elisa plate and a plurality of liquid bottles of an existing kit for detecting the tumor markers are easily damaged due to collision is solved.

The utility model discloses a kind of kits for multiple tumor marker detection, it is related to medical instrument technical field, including box and sample liquid preparation component, the box one end is equipped with receiving groove, the sample liquid preparation component is set in receiving groove inside, the sample liquid preparation component includes the needle cylinder being set to outside, needle cylinder inside is filled with blood sample diluent in advance.This application provides a kind of kits for multiple tumor marker detection, the blood sample of examinee is injected by needle cylinder one side middle part injection port using syringe, and mixed with the blood sample diluent filled in advance in needle cylinder, operator handheld needle cylinder and through the cooperation of rotating shaft sleeve and receiving groove side wall connecting port, swing needle cylinder so that blood sample and diluent are mixed uniformly, squeeze needle handle again, and sample liquid flows to detection area through distribution flow channel, the sample liquid preparation component is integrated in the side of box in this application, it is convenient for user to prepare blood sample quickly, and it is more practical.

The application discloses an engineering bacterium, a biosensor and a detection method thereof. The biosensor takes the modified Acinetobacter baylyi ADP1 as a carrier, utilizes the natural nucleic acid uptake capacity thereof, and can autonomously absorb exogenous nucleic acid without external induction; after the nucleic acid is internalized, the hairpin structure restriction of the blocked gRNA is removed, the CRISPRa system is activated, the dCas9-SoxS protein is targeted and combined with the target promoter, the CymA-MtrCAB gene expression is started, and the specific recognition of the nucleic acid sequence is realized. The system integrates the CymA-MtrCAB transmembrane electron conduction module, converts the biological recognition signal into an electrochemical signal output in real time through the membrane protein-mediated electron transfer chain, and establishes a detection path of 'nucleic acid recognition-gene activation-electric response'. The biosensor has the advantages of modular structure, high response specificity, high detection sensitivity, wide environmental adaptability and the like, is suitable for tumor marker detection, wearable health monitoring and other biological molecule recognition scenes, and provides a novel programmable biological perception solution with high universality.

The invention belongs to the technical field of marker detection, and discloses a multichannel Raman spectrum analysis system for tumor marker detection, which comprises a preprocessing module, an acquisition module, an enhancement processing module, an analysis module and an output module, the pretreatment module is used for uniformly coating the surface of a target tissue slice with an electromagnetic nano material; the acquisition module is used for acquiring a Raman spectrum of a target tissue slice, and the Raman spectrum comprises a plurality of Raman scattering lights with different wavelengths; the enhancement processing module is used for converting the Raman spectrum acquired by the acquisition module into an electric signal, and is also used for enhancing the electric signal; the analysis module is used for identifying a tumor marker by analyzing the enhanced electric signal; and the output module is used for marking a tumor marker according to an identification result of the analysis module, and is also used for outputting a target tissue pathological image marked with the tumor marker.

The present invention belongs to the field of biomedical technologies. The present invention discloses a comprehensive analysis platform for tumor marker detection data, which includes: a data collection module for collecting marker detection data of candidate markers; a data encoding module for encoding the marker detection data to obtain marker encoded data; a model construction module for constructing a tumor prognosis model based on the marker encoded data; a potential evaluation module for evaluating the potential of markers according to the marker encoded data and the tumor prognosis model; and a marker determination module for determining whether a candidate marker can be used as a tumor marker according to the marker potential. If so, a quantitative calculation of clinical practicability is performed. The present invention not only improves the detection and verification efficiency of tumor markers, but also provides important support for personalized medicine and precision treatment, and can significantly improve the prognosis evaluation and treatment effect of patients.

The invention relates to the technical field of tumor marker detection, in particular to a tumor sampling and tumor marker detection system. The method comprises the following steps: collecting a concentration value; after the patients are classified, the patients are clustered based on concentration values, and abnormity of each tumor marker is determined; analyzing all detected tumor markers to obtain progress values of the tumor markers; analyzing the fluctuation condition based on the progress value, and calculating a risk assessment index according to the fluctuation condition proportion and the fluctuation index of the patient in the same kind; determining the next interval duration through the risk assessment index; and detecting the tumor marker again after the adjusted interval duration. According to the invention, the problem of time misjudgment caused by false progress is solved.

This invention discloses a tumor marker detection system based on an antibody-modified gold nanoparticle periodic linear array, belonging to the field of biomolecular detection. In this invention, a primary antibody is modified onto gold nanowires, and a nanolabel of antibody molecules modified onto gold nanoparticles is prepared as a detection tag (secondary antibody) for tumor markers. A test solution mixed with the secondary antibody is drop-coated onto the structure, and after a sufficient reaction time, the entire structure is rinsed. When light is incident perpendicularly on the gold nanowire array, surface plasmon resonance (SPR) occurs. The tumor markers (antigens) present in the solution are specifically adsorbed with the primary and secondary antibodies, forming a double-antibody sandwich structure, which changes the macroscopic color of the structure. By observing whether the color changes in different regions of the nanowire array after detection, the presence of tumor markers in the test solution can be determined. This invention's system can simultaneously detect multiple tumor markers and features short detection time, small size, low cost, high sensitivity, and ease of outdoor observation.

The invention relates to the technical field of photochemical biosensing detection, and discloses a fluorescent biosensor, system and method for detecting tumor markers, commercialized filter paper is used as a substrate, a silica-based sensitive layer is constructed on the surface of the substrate by a sol-gel method to obtain a nanopore membrane, and specific capture of target molecules is realized by introducing click chemistry. The stability and the tolerance of a sensing system are improved. The method is simple and convenient to operate, high in result visualization degree, capable of greatly shortening the detection time, high in specificity, high in sensitivity, high in precision, good in reproducibility and low in cost, and can be widely applied to tumor marker detection.

The present invention discloses a method for detecting tumor marker PD-L1 using a composite structure optical fiber sensor based on gold nano-urchins, comprising the following steps: preparing a pSC4 solution, immersing the NS-LSPR-FOS in the pSC4 solution for 12 hours; washing to remove unbound pSC4 molecules; immersing the sensor end face in a PBS solution containing 20 μg / mL PD-L1 antibody for 1 hour to form a specific recognition layer; immersing the sensor end face in a BSA solution for 20 minutes; successively immersing the sensor end face in PD-L1 antigen solutions of different concentrations, with each group being immersed for 45 minutes, from low to high concentration; recording the reflection spectrum every 15 seconds through a spectrometer to track the movement of the resonance valley wavelength and detect the PD-L1 concentration. Compared with liquid biopsy methods such as ELISA, IFA, and microfluidic chips, this application has many advantages such as small size, label-free, real-time detection, and in-vivo detection, and is expected to overcome the two major challenges of low-concentration detection and spatio-temporal heterogeneity characterization of tumor marker PD-L1, playing an important role in cancer research and immunotherapy.

The invention relates to the technical field of tumor marker detection, in particular to a sensor for detecting a tumor marker EpCAM and a preparation method and application of the sensor. The sensor is obtained by self-assembling a compound shown in a formula 1) and a compound shown in a formula 2) to obtain diacetylene liposome, and then combining a fluorescent dye labeled EpCAM aptamer with the diacetylene liposome through electrostatic interaction. The sensor disclosed by the invention can be used as an activatable fluorescent sensor for sensitively and selectively detecting EpCAM, has a quick response effect, and can realize quick and sensitive analysis and detection of EpCAM in an aqueous solution, a living cell level and an animal level; # imgabs 0 # formula (1) and # imgabs 1 # formula (2).

The invention relates to the technical field of electrochemical biosensing detection, and discloses a nanopore biosensor, system and method for detecting tumor markers. The carbon fiber paper is used as a substrate, so that excellent mechanical strength and conductivity are provided; a silicon-based sensitive layer with a nano-scale pore structure is constructed on the surface of the substrate by a sol-gel method, so that the comprehensive regulation and control of the pore diameter are realized; click chemistry is introduced to realize specific capture of target molecules, and the stability and tolerance of a sensing system are improved. The method for detecting the tumor marker by combining the nanopore membrane with click chemistry has universality and universality, is simple and convenient to operate, greatly shortens the detection time, is strong in specificity, high in sensitivity, high in precision, good in reproducibility and low in cost, can be widely applied to detection of the tumor marker, and has a wide application prospect. And the application of commercial carbon fiber paper in the field of life analysis is expanded.

The invention belongs to the technical field of tumor marker detection, and particularly relates to a method for detecting a tumor marker based on magnetic separation and super-hydrophobic convergence strategy SERS (Surface Enhanced Raman Scattering). A strategy of magnetic separation capture-probe separation-probe convergence is adopted, the magnetic balls capable of capturing the tumor markers in the sample are added, the tumor markers are rapidly extracted from the sample through magnetic separation, and the operation is simple and convenient; adding an SERS probe capable of being specifically combined with the marker, and separating the Raman probe on the surface of the tumor marker from the magnetically separated sample by adopting a desorption process; finally, the desorbed Raman probes are converged through the super-hydrophobic SERS substrate, Raman signals are collected, the method has the advantages of being high in specificity and sensitivity, good in reproducibility and the like, the sensitivity and reproducibility of SERS detection of tumor markers are improved, and the bottleneck problem that an existing detection method restricts actual application of SERS in early screening of the tumor markers is solved.

The invention belongs to the technical field of biological nanotechnology, and particularly relates to a DNA triangular prism support structure and a DNA enzyme walker guiding directional movement based on a DNA triangular prism support track. The six vertexes of the constructed three-dimensional DNA triangular prism scaffold structure can be used for designing specific cohesive ends and fixing at least three different substrate chains and a DNAzyme walker, and the structural characteristics enable DNAzyme to perform sequential cutting according to the principle of the shortest spatial distance between substrates under the assistance of Mg, so that the stability of the scaffold structure is improved. According to the system, the derailing phenomenon in a traditional free system is completely avoided through the physical confinement effect, and the cutting path can be dynamically adjusted when an adjacent substrate is removed. The technical scheme has the advantages of low preparation cost, strong structure programmability, high cutting specificity, controllable operation direction and the like, and can be widely applied to the biomedical fields of tumor marker detection, targeted drug delivery, cancer precise treatment and the like.

This invention discloses an electrochemiluminescence microfluidic chip for the detection of multiple tumor markers, relating to the field of biodetection technology. It includes a substrate and a microfluidic channel system, an electrochemiluminescence detection unit, and a signal acquisition interface integrated on the substrate. The microfluidic channel system includes a sample inlet, a shunt structure, multiple parallel and equally spaced detection channels, and a waste liquid pool connected in sequence. The shunt structure includes a main channel and multiple branch channels integrally formed with the main channel. In this invention, the microfluidic channel system, the electrochemiluminescence detection unit, and the signal acquisition interface are integrated on the same substrate, achieving an integrated design for sample transmission, reaction, detection, and signal export. Compared to traditional distributed detection technologies, this significantly simplifies the detection operation steps, reduces the professional skills required of operators, and shortens the detection cycle, meeting the needs of rapid clinical diagnosis.

The invention discloses a tumor marker detection method and system based on DNA quantitative analysis in the technical field of cell images. The detection method comprises the following steps: S1, staining a tissue / cell section; s2, scanning the stained tissue / cell section to obtain a digital section image; s3, performing cell segmentation on the slice image to obtain a segmented image containing a cell mask region, and performing DNA quantitative analysis and cytological analysis and classification on the segmented image; s4, performing virtual dyeing on the slice image; and S5, acquiring a DNA quantitative analysis result and a cytological analysis and classification result, and jointly outputting the results in combination with the virtual dyeing image. According to the detection method, a complete solution with high objectivity and high clinical practicability is jointly formed through collaborative diagnosis of DNA quantitative analysis and cytological analysis and by combining virtual staining, the collaborative diagnosis efficiency is generated, and the problem that practical application is difficult due to the fact that current pathological AI diagnosis is insufficient in accuracy is solved.

The invention discloses a marker group for screening and diagnosing ovarian cancer and application of the marker group, and belongs to the technical field of molecular biology. The marker group comprises at least four of phospholipids and polypeptides as shown in sequences SEQ ID NO. 1-41. When being used for auxiliary diagnosis and early screening of ovarian cancer, the marker combination constructed by the invention has more excellent sensitivity and specificity, and the auxiliary diagnosis results of the marker combination are partially or completely superior to those of an ROMA index ovarian cancer risk prediction model recommended by a current clinical guide. The early screening result is also partially or completely superior to that of tumor marker CA125 detection and HE4 detection with the highest ovarian cancer early screening value at present. The kit is expected to be applied to diagnosis and treatment of ovarian cancer.

The application belongs to the technical field of biological detection, and particularly relates to a magnetic bead and a method for detecting glycosylation tumor marker. The magnetic bead for detecting the glycosylation tumor marker is a boronic acid-based magnetic bead, and the magnetic microspheres on the surface of the magnetic bead contain boronic acid groups, the size of the magnetic microspheres is 300-500 nm, and the content of boron elements on the surface of the magnetic microspheres is greater than or equal to 1000 mg / g. Specifically, the boronic acid-based magnetic bead is used as a solid-phase extraction agent, and the method comprises the following steps: the combination of the magnetic bead and the glycosylation tumor marker, the blocking of the magnetic bead, and the coupling of the corresponding antibody of a signal molecule label, and the detection of the magnetic bead on a corresponding signal molecule detection instrument after magnetic separation. The application has the advantages of simple route, rapid and convenient operation, low cost and the like for detecting the glycosylation tumor marker.

The application discloses a marker group for ovarian cancer screening and diagnosis and application thereof, and belongs to the field of molecular biological technology.The marker group comprises at least four of phospholipids and polypeptides shown in sequences SEQ ID NO.1-41.The marker group constructed by the application has more excellent sensitivity and specificity when used for the auxiliary diagnosis and early screening of ovarian cancer, and the auxiliary diagnosis results are all partially or completely superior to the ROMA index ovarian cancer risk prediction model recommended by the current clinical guideline.The early screening result is also partially or completely superior to the detection of the tumor marker CA125 and the detection of HE4, which are the most valuable tumor markers for the early screening of ovarian cancer, and the application is expected to be applied to the diagnosis and treatment of ovarian cancer.

The application belongs to the technical field of electrochemiluminescence immunosensor, discloses a kind of electrochemiluminescence immunosensor for detecting tumor marker and application thereof, and the preparation system of electrochemiluminescence immunosensor for detecting tumor marker includes: graphite carbon nitride preparation module, working electrode pretreatment module, central control module, antigen standard solution preparation module, modified electrode preparation module, immunosensor preparation module, tumor marker detection module, sensor detection curve drawing module, data storage module, update display module.The electrochemiluminescence immunosensor for detecting tumor marker provided by the application has high analysis sensitivity, strong specificity, is simple to use and low in cost.The application improves the sensitivity of electrochemiluminescence immunosensor by dropping coating bovine serum albumin solution and tumor marker antigen standard solution on the surface of graphite carbon nitride working electrode, and greatly simplifies the electrode preparation process without enzyme and label.

The invention belongs to the field of tumor marker detection and application, and particularly relates to an electrochemiluminescence detection kit based on a lanthanide complex and a preparation method, the lanthanide complex Tb-dCOP constructed according to a specific molar ratio is used as a core electrochemiluminescence material, the lanthanide complex Tb-dCOP has relatively high conductivity and stability, can generate an extremely strong ECL signal, and can be used for detecting tumor markers. According to the kit, AgNPs (at) ZnONFs are used as a co-reaction reagent and a signal stabilizer and are combined with a DNA chain fixing carrier, an efficient sensing interface is constructed, ultra-sensitive detection of microRNA-21 (miRNA-21) is achieved through a dendritic double-chain hybridization chain reaction (D-HCR), and the detection limit reaches 46.8 amol / L. Compared with the prior art, the kit has the advantages that the sensitivity is high, and the sensitivity is high. The invention effectively overcomes the problems of low sensitivity and poor accuracy in detection of tumor markers such as miRNA-21 and the like in the prior art, and has the advantages of lower detection limit, better stability, wider linear range and the like.

The embodiment of the invention provides a tumor marker detection method and related equipment, and belongs to the technical field of medical detection. The method comprises the following steps: modifying corresponding antibodies on a preset sensing chip according to a plurality of different tumor marker antibodies to construct a preset micro-fluidic chip; wherein the preset micro-fluidic chip comprises a micro-fluidic channel, and the micro-fluidic channel comprises a reference region and a sensing structure corresponding to each tumor marker antibody; inputting a to-be-detected blood sample into the micro-fluidic channel, and dynamically collecting to obtain chip detection video data; performing image analysis according to the chip detection video data to obtain preset gray scale change data, and further calculating the preset gray scale change data to obtain marker concentration data; wherein the preset gray scale change data comprises the gray scale change data corresponding to the reference area and each sensing structure. According to the embodiment of the invention, high-sensitivity quantitative detection can be realized, and the accuracy and efficiency of diagnosis are effectively improved.

The present invention belongs to the technical field of tumor marker detection, and specifically relates to a method for detecting tumor markers by SERS based on magnetic separation and superhydrophobic aggregation strategy. The present invention adopts the strategy of "magnetic separation capture → probe separation → probe aggregation", adds magnetic beads capable of capturing tumor markers in the sample, quickly extracts tumor markers from the sample through magnetic separation, and has simple operation; then adds SERS probes that can specifically bind to the markers, and then adopts a desorption process for the sample after magnetic separation to separate the Raman probes on the surface of the tumor markers; finally, the desorbed Raman probes are aggregated by a superhydrophobic SERS substrate, and Raman signals are collected. This method has the advantages of strong specificity, high sensitivity, good reproducibility, etc., improves the sensitivity and reproducibility of SERS detection of tumor markers, and solves the bottleneck problem that existing detection methods restrict the practical application of SERS in the early screening of tumor markers.