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Detection type gene chip for detecting various infectious desease and use thereof

A gene chip, infectious disease technology, applied in the field of medical testing and gene chip, detection type gene chip

Inactive Publication Date: 2005-03-30
陶开华 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, domestic research on practical microarrays has just started. Up to now, there have been no domestic and foreign applications for the seven important infectious diseases in my country (epidemic hemorrhagic fever, cholera, E. coli O157:H7, malaria, scrub typhus, and Leptospira). , schistosome) rapid detection gene chip

Method used

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  • Detection type gene chip for detecting various infectious desease and use thereof
  • Detection type gene chip for detecting various infectious desease and use thereof
  • Detection type gene chip for detecting various infectious desease and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Example 1. Development and preliminary application of a gene chip for detection and typing of malaria parasites

[0066] Malaria is a parasitic disease that seriously endangers human health. Especially in the event of floods, large-scale crowd gatherings and bioterrorism, the threat of epidemics is still relatively prominent. Efficient, sensitive, and rapid methods of monitoring malaria parasites are the primary prerequisite for malaria control. We established a gene chip for detecting and typing Plasmodium vivax and Plasmodium falciparum, optimized and determined the detection conditions of the chip, and compared some samples.

[0067] 1. Design of primers and probes

[0068] On the basis of fully consulting various literatures, according to the small subunit ribosomal ribonucleic acid (SSrRNA) of Plasmodium vivax and Plasmodium falciparum, the corresponding sequences of other human Plasmodium genera such as Plasmodium malariae and human SSrRNA were analyzed and compa...

Embodiment 2

[0118] Example 2. Preparation and detection of Schistosoma japonicum gene chip

[0119] Schistosomiasis is a zoonotic parasitic disease that is widely distributed all over the world, and the epidemic is still very serious in some areas of my country. In the process of prevention and control, the reinfection of the population and the re-population of schistosomiasis in the control area have always been difficult problems in the practice of prevention and control. Since oncomelania is the only intermediate host of Schistosoma japonicum, the spread and redistribution of Oncomelania snails are the basic factors causing the re-epidemic of schistosomiasis. Therefore, efficient and correct monitoring of schistosomiasis and snails is extremely important in the prevention and control of schistosomiasis. Gene chip technology is suitable for large-scale and high-throughput detection requirements, especially for on-site detection and monitoring of schistosomiasis endemic areas, and has b...

Embodiment 3

[0131] Example 3. Establishment of a method for detecting Orientia tsutsugamushi DNA by gene chip technology

[0132] Scrub typhus is an insect-borne natural foci disease caused by Orientia tsutsugamushi. In recent years, the epidemic area in my country has been expanding. Because the disease is prone to misdiagnosis, resulting in improper treatment, and even death. Therefore, rapid and accurate diagnosis is extremely important. We selected the 56kD protein gene sequences of three standard strains of Orientia tsutsugamushi, which are both conserved and specific, synthesized oligonucleotide probes, and preliminarily established a diagnostic method for detecting Orientia tsutsugamushi-specific DNA using chip technology

[0133] 1. Design and synthesis of primers and probes

[0134] According to the sequence of the main surface antigen 56kD protein of Orientia tsutsugamushi standard strains Karp Gilliam and Kato[2,3], combined with computer software analysis and NCBI BLAST scre...

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Abstract

The present invention relates to a gene chip for detecting several infections lideases and its application. It can be used for detecting, typing and strain-identifying 7 main infections diseases of epidemic hemorrhagic fever, tsutsugamushi disease, leptospirosis, schistosomiasis, malaria, cholera and hemorrhagic enteritis due to 0157:H7. Said invention can select specific gene probe and PCR primer respectively from S gene of epidemic hemorrhagic fever virus, 56KD protein gene of oriental rickettsia, 23 SRNA gene of leptospirosis, 5D antigen gene of schistosomiasis, SSrRNA gene of malarial parasite, 0157 antigen gene of colibacillus 0157:H7, H7 antigen gene and toxic gene and outer membrane OWP protein gene of cholera vibrio and toxic gene.

Description

technical field [0001] The invention relates to a detection type gene chip for detecting various infectious diseases and the technical field related to the application is medical inspection and gene chip technology. It is a gene chip for the detection of infectious diseases. It detects and types seven major infectious diseases: epidemic hemorrhagic fever, scrub typhus, leptospirosis, schistosomiasis, malaria, cholera, and O157:H7 hemorrhagic enteritis. Identify strains. Background technique [0002] Epidemic hemorrhagic fever, scrub typhus, leptospirosis, schistosomiasis, malaria, cholera, and hemorrhagic enteritis (Escherichia coli O157:H7) are still the main infectious diseases that are prone to epidemics in my country and pose a great threat to human health. Additionally, these pathogens are susceptible to biological warfare agents. [0003] Therefore, the rapid detection and determination of the types of these pathogens not only has important clinical value and provides...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
CPCY02A50/30
Inventor 陶开华李越希张锦海
Owner 陶开华
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