59 results about "CSF - Cerebrospinal fluid" patented technology

This invention belongs to the field of biosensing and detection technology, specifically disclosing a Tau protein electrochemiluminescent aptamer sensor, its preparation method, and its application. The sensor uses a CsPbBr₃@PVP / Au composite material as the electrochemiluminescent substrate. A Tau protein-specific aptamer is immobilized on the substrate surface via Au-S bonds, and non-specific adsorption sites are blocked with 6-mercapto-1-hexanol. The CsPbBr₃@PVP / Au composite material is prepared by encapsulating CsPbBr₃ with PVP and then combining it with Au NPs. This invention utilizes the coordination passivation effect of PVP and CsPbBr₃ to significantly improve the aqueous stability and electrochemiluminescence efficiency of perovskite nanocrystals. Combined with the anchoring effect of AuNPs and the specific recognition effect of aptamers, it achieves ultrasensitive detection of Tau protein with a detection limit as low as 0.80 fg / mL. Furthermore, it exhibits excellent selectivity, stability, and detection accuracy in complex biological samples such as cerebrospinal fluid, providing a novel and efficient detection platform for the early diagnosis of Alzheimer's disease and showing promising clinical application prospects.

Disclosed are methods for quantification of a perivascular space in a subject's central nervous system. The methods include administering contrast agent into cerebrospinal fluid of the subject; performing quantitative ultra-short time-to-echo contrast-enhanced magnetic resonance imaging (QUTE-CE MRI) on a region of interest of the subject's brain; and determining presence of the contrast agent in the perivascular space within the region of interest.

This utility model discloses an anesthesia puncture needle, including an outer needle tube and a needle hub connected to one end of the outer needle tube; it also includes an inner needle body nested inside the outer needle tube, with one end extending to the outside through the opening of the outer needle tube, and the other end extending into the needle hub. The protruding end of the inner needle body is conical, and at least one collection hole is opened on its outer wall near the end. This utility model relates to the field of medical anesthesia technology. This anesthesia puncture needle, through the cooperation of the inner needle body and the outer needle tube, can facilitate the puncture needle to enter the subarachnoid space. At the same time, it can pull the piston collector to draw cerebrospinal fluid from the end of the inner needle body and store it in the internal cavity of the piston collector, so that it can be mixed with the colorimetric solution injected through the colorimetric branch tube, thereby enabling colorimetric treatment of the cerebrospinal fluid, facilitating rapid diagnosis by medical personnel. In this process, the colorimetric solution will not come into contact with the human body, avoiding harm to the human body.

The application discloses a cistema brain-computer interface-minimally invasive whole brain nerve signal acquisition and closed-loop control system based on a cerebrospinal fluid channel and a method thereof. The system comprises a flexible cistema interface array, an ultra-wideband wireless energy and data synchronous transmission module, a nerve signal analysis and decoding module, a self-adaptive closed-loop control module and a system integrated control platform. The flexible cistema interface array is implanted in a cerebrospinal fluid cavity in a minimally invasive manner to acquire multi-brain region nerve signals; and the ultra-wideband transmission module realizes energy supply and high-speed bidirectional communication between an implanted body and an extracorporeal device. The application solves the problems of a large trauma, limited coverage and poor signal quality of an existing invasive interface, has the advantages of minimally invasive, wide area coverage, high signal quality and good integration, and is suitable for diagnosis and treatment of neurological diseases such as epilepsy suppression and post-stroke motor function reconstruction.

The application relates to application of BDNF as a biomarker in prenatal prediction of postpartum depression morbidity risk. The mBDNF and proBDNF levels in cerebrospinal fluid and plasma can be used as independent markers for prediction of postpartum depression morbidity risk, and the plasma mBDNF or cerebrospinal fluid proBDNF level can be used for prediction of postpartum depression morbidity risk through prenatal detection, which is helpful for prediction, early identification and timely intervention of postpartum depression and reduction of harm.

The application belongs to the technical field of medical devices, and particularly discloses an intracranial pressure monitoring and cerebrospinal fluid drainage integrated nursing device, which comprises a mounting frame, a collecting mechanism is arranged on the mounting frame and used for collecting cerebrospinal fluid, a drainage tube is connected with the collecting mechanism through a three-way valve and used for draining cerebrospinal fluid, and a monitoring mechanism is arranged on one side of the collecting mechanism and connected with the drainage tube through the three-way valve. Through the action of the metal wire, the outer protective sleeve, the first moving sleeve and the second moving sleeve, the fixed tube between the drainage tube and the puncture tube can be supported and protected on the inner side and the outer side at the same time, the pipeline has the characteristics of resisting hard bending and resisting external compression or pulling, even if the fixed tube is pressed and slightly pulled, the lumen of the fixed tube will not collapse and form a hard bending angle, and the lumen can still remain open, so that the small movement of the head of the patient is not affected, and the monitoring and drainage effect is improved.

The application relates to the technical field of image processing, in particular to an MRI image feature extraction method for dynamic evaluation of child brain development, which comprises the following steps: determining a cerebrospinal fluid coefficient of a child brain; dividing an MRI image into a cerebrospinal fluid region and a residual brain region based on the cerebrospinal fluid coefficient of the child brain of each pixel point; determining a white matter coefficient of each pixel point in the residual brain region according to the gray value of each pixel point in the decayed weighted image of each pixel point in different gradient directions, and the gray value of each pixel point in the first weighted image and the second weighted image in the residual brain region; segmenting the white matter region and the gray matter region of the residual brain region based on the white matter coefficient, and extracting image features in the white matter region and the gray matter region. In this way, the accuracy and reliability of the segmentation result of different tissue regions in the child brain MRI image are improved.

The application provides a biomarker dopamine-3-O-sulfate (DA3S) for evaluating the risk of movement complications of Parkinson's disease patients. The application also provides a cerebrospinal fluid kit for evaluating the risk of movement complications of Parkinson's disease patients. The application is based on the concentration of DA3S in cerebrospinal fluid, and combines with clinical variables such as daily dose of levodopa (LEDD), disease duration, etc. to construct a joint prediction model, so as to realize early and quantitative individualized evaluation of the risk of movement complications of Parkinson's disease patients. The application also provides a plasma kit and a urine kit for evaluating the risk of movement complications of Parkinson's disease patients.

This invention discloses the application of the serum biomarker miR-206 in the preparation of Alzheimer's disease diagnostic products. This invention is the first to use the serum biomarker miR-206 in the effective and auxiliary diagnosis of Alzheimer's disease, demonstrating extremely high diagnostic performance. This invention lays the technical and data foundation for the development of stable and reliable Alzheimer's disease point-of-care testing kits or automated chemiluminescence detection systems and other in vitro diagnostic products. It has significant clinical application prospects in promoting the transformation of early screening and auxiliary diagnosis of Alzheimer's disease from reliance on complex imaging and cerebrospinal fluid examinations to convenient, non-invasive blood tests.

The present application provides a kind of nutritional composition and its application in the preparation of protecting brain barrier health product.The nutritional composition provided by the present application includes animal bifidobacterium lactis subsp CP-9 and 3'-sialyllactose, the present application has been confirmed from histological level: the expression of occludin and claudin-5 in brain tissue can be improved after the nutritional composition of animal bifidobacterium lactis subsp CP-9 and 3'-sialyllactose is applied to organism, so as to strengthen the integrity and occlusion of brain barrier structure, promote the maturation and function perfection of blood-brain barrier, cerebrospinal fluid barrier and other brain barrier system, to realize the effect of enhancing brain barrier function, promoting brain barrier development and other maintenance brain barrier health.

The application discloses a RPA-CRISPR / Cas12a-based rapid visual detection method for manning's paragonimus. The method takes the mitochondrial cox1 gene as a target, screens a conservative region through sequence alignment, designs specific crRNA and RPA primers, optimizes Cas12a, crRNA, a fluorescent reporter molecule and a buffer system, and constructs a recombinase polymerase amplification combined CRISPR / Cas12a trans-cleavage detection system. Two visual detection strategies, visual fluorescence and lateral flow chromatography, are adopted, so that instant detection can be realized without large instruments and professional personnel, and the method has the advantages of high sensitivity and good specificity, can accurately detect manning's paragonimus DNA, cat and dog feces and blood samples, and brain schistosomiasis cerebrospinal fluid samples, and is suitable for on-site rapid screening.

This utility model discloses a lumbar puncture needle with monitoring function, comprising: a needle body, with a puncture needle main body internally connected to the needle body. The puncture needle main body is used to detect puncture depth and monitor cerebrospinal fluid pressure. The puncture needle main body includes an outer needle and an inner needle, with the inner needle located inside the outer needle. A gap is reserved between the outer and inner needles. A signal receiving mechanism is provided at the top of the outer needle. Both the outer and inner needles are electrically connected to the signal receiving mechanism via a point-to-line connection. This utility model provides a lumbar puncture needle with monitoring function, consisting of a system and a puncture needle main body. It can issue an alarm when the puncture needle tip contacts the cerebrospinal fluid in the subarachnoid space of the lumbar spine, and can monitor the cerebrospinal fluid pressure in real time during cerebrospinal fluid collection. Through precise puncture depth feedback and real-time cerebrospinal fluid pressure monitoring, doctors can more accurately locate the puncture point, reduce surgical errors, and improve the success rate of surgery. Real-time monitoring of cerebrospinal fluid pressure helps to detect abnormal intracranial pressure in a timely manner, prevent serious complications such as brain herniation, and ensure patient safety.

The present application relates to neuromodulation and brain-computer interface technical field, propose a kind of robust optimization method and system under the condition of parameter uncertainty, robust optimization method includes constructing five-layer head tissue model including skin, skull, cerebrospinal fluid, grey matter and white matter based on the magnetic resonance image of patient, and the independent conductivity uncertainty radius of each layer of tissue is set, multi-dimensional ellipsoid uncertainty set is constructed;Min-Max worst case cost function is constructed and is solved, guarantee in ellipsoid set under the worst case, electrode current weight meets safety constraint;Worst case cost function is converted into second-order cone programming form using dual principle;Pseudo-trace perception sliding window mechanism is introduced to monitor the continuity of physiological signal or impedance data, when detecting non-physiological mutation, trigger re-optimization by calling historical stable parameters and amplifying uncertainty radius;When re-optimization has no solution, trigger safety derating mechanism, and the current instruction of previous period is scaled proportionally.

This invention discloses a cerebrospinal fluid external filtration and dialysis device for the purification and treatment of cerebrospinal fluid in diseases such as central nervous system infections and subarachnoid hemorrhage. The device includes a dual-lumen drainage tube, comprising an outflow pipe and an inflow pipe. The outflow pipe is connected to the inlet of the filtration and dialyzer via a suction-side connecting pipe. The suction-side connecting pipe passes through a peristaltic pump and is equipped with a sampling pipe, on which a detachable timed sampler is mounted. The first outlet of the filtration and dialyzer is connected to a storage bag via a suction connecting pipe, and the second outlet is connected to the inflow pipe via a return connecting pipe. Pressure sensors are installed on both the suction-side and return connecting pipes, and a flow meter is installed on the return connecting pipe. These components form a feedback control loop with a control processor to adjust the pump speed and valve opening and closing, achieving safe and controllable flow and pressure. This device can filter / dialyze pathogens and inflammation-related substances in cerebrospinal fluid and return them, while simultaneously performing timed sampling for dynamic monitoring.

The application discloses a cerebrospinal fluid low-abundance protein biomarker for multiple sclerosis diagnosis and application thereof, and belongs to the field of biomedical technology. The application obtains the MS cerebrospinal fluid biomarker with high specificity and sensitivity through high-throughput proteomics technology screening and strict clinical verification, and establishes a standardized detection method, thereby providing a new core biomarker PFKP protein for the diagnosis of MS, enriching the technical means for early diagnosis of MS, and having good market application prospect and social and economic benefits.

The present invention relates to a device for reading multiple cerebrospinal fluid droplets equipped with a micro-drip mechanism and a reading mechanism with a movement mechanism for moving along the X- and Y-axes by mean of worm gears, rails and motors. The device for reading multiple cerebrospinal fluid droplets of the present invention uses optical fibre and a light source to read the microdroplet that is placed on microscope slides located on a base. The device for reading multiple cerebrospinal fluid droplets of the present invention has a moisture-retaining structure configured to maintain moisture inside the device and prevent the microdroplets placed on the slides (3) from evaporating.

This invention discloses a gene biomarker panel for detecting PCNSL based on cerebrospinal fluid (cerebrospinal fluid) ctDNA, relating to the fields of gene detection and molecular diagnostics. The gene biomarker panel includes MYD88, PIM1, CD79B, GNA13, IRF4, DTX1, KMT2D, and B2M. By detecting the mutation status of these genes in a subject's cerebrospinal fluid sample, the mutation status is input into a trained random forest classification model to generate auxiliary judgment results, providing molecular evidence for diagnosing PCNSL in the subject. This eight-gene combination panel has shown high diagnostic specificity in multiple cerebrospinal fluid ctDNA-based validation cohorts and has the ability to identify MYD88 wild-type PCNSL.

This invention discloses a disposable closed-loop lumbar puncture pressure measurement and sampling integrated device and its pressure measurement method. The device includes: a first Luer interface connected to a lumbar puncture needle or lumbar drainage catheter, a second Luer interface connected to a sampling container, and a valve assembly disposed between the two interfaces. The valve assembly has a pressure measurement path, a sampling path, and a closing path, and can achieve multi-position switching through a stopcock or slide valve. The pressure measurement path is connected to a pressure indicator component, which transmits pressure through a diaphragm coupled to the cerebrospinal fluid (CSF) but is not directly connected to the CSF. The pressure indicator component can display the CSF pressure within a range of 0-60 cm H2O. The valve assembly preferably has a bubble trapping chamber and / or a flow limiting structure to reduce the influence of air bubbles on the reading and reduce the risk of rapid fluid release due to misoperation. This invention can achieve closed-loop pressure measurement without a long tubing column in the lateral decubitus position, and can complete CSF sampling within the same sterile closed loop, reducing the chance of external contamination, reducing sample waste, and simplifying the operation process.

The present invention provides a novel method to isolate and expand pure neural stem cells (NSCs) from cerebrospinal fluid (CSF) of premature babies with Intraventricular haemorrhage, which produces a population enriched in NSC-CSF cells free of contaminating fibroblasts and other cell types. The present invention also includes substantially pure populations of CSF-NSC cells, and their use to treat and prevent diseases and injuries, including Intraventricular haemorrhage and post- hemorrhage hydrocephalus / developmental deficits.

The utility model discloses a kind of cerebrospinal fluid cryopreservation tubes, including outer tube and inner tube, two cavities along outer tube center line are equipped in outer tube, one is centrifugal tube cavity, another is cryopreservation tube cavity, inner tube is used to hold cerebrospinal fluid, can be placed in any cavity, inner tube cover and outer tube cover are detachably connected, micropore that can filter supernatant is opened in inner tube bottom portion.The utility model has the dual function of centrifugal tube and cryopreservation tube, and the cell precipitate (cerebrospinal fluid has type component) after centrifugation is preserved together with supernatant, and it is convenient to use.

Phosphorylated human tau protein at amino acid serine 413 (pS413 tau) can serve as a biomarker for tauopathies such as Alzheimer's disease. Detecting and quantifying pS413 tau in biological samples such as cerebrospinal fluid can be used to develop therapies for certain tauopathies. However, pS413 tau is present at very low levels in biological samples. Accordingly, the present invention provides a highly sensitive assay method for detecting and quantifying pS413 tau in biological samples comprising a series of steps described herein.

The present invention relates to a multispecific antibody which binds to human serum albumin (HSA) and to a second antigen, wherein the apparent affinity of the antibody to the second antigen in the presence of the average concentration of HSA in human cerebrospinal fluid is higher compared to the affinity of the antibody to the second antigen in the presence of the average concentration of HSA in human peripheral blood.

The application discloses discovery and application of desmin autoantibody as a marker of nervous system diseases. The application finds that only the fluorescent signal existing in the nervous system disease patients but not in the healthy people, and the fluorescent mode of the IgG related autoimmune antibody in the mouse brain tissue section is different, which indicates that the patient may have autoimmune antibody. Through the immunoprecipitation technology, the IgG specific protein related to the nervous system disease is sought, the specific desmin autoantibody is found by adopting the CBA mode, and then the desmin autoantibody is verified in the cerebrospinal fluid, so that it is finally determined that the desmin autoantibody can be used as a specific marker for diagnosing the nervous system autoimmune diseases. The expression of the desmin autoantibody is detected by taking the desmin as a detection antigen, and the detection of the desmin autoantibody can be applied to a kit for detecting the desmin autoantibody, and can be applied to the detection of the nervous system diseases, so that the marker for identifying the nervous system diseases is enriched, and the accuracy of the detection of the nervous system related diseases is improved.

This invention discloses a dual-recognition electrochemical sensor, its preparation method, and its application. The sensor comprises: (1) a working electrode modified with a molecularly imprinted polymer membrane; and (2) a vancomycin-modified copper-based metal-organic framework nanoenzyme signal probe. This signal probe specifically binds to the cell wall of Gram-positive bacteria and catalyzes the oxidation of o-phenylenediamine by peroxide to generate an electrochemical signal. During detection, the target bacteria are first captured by the molecularly imprinted layer, and the signal probe binds to the bacterial cell via vancomycin, forming a "sandwich" structure. The change in current signal is detected by differential pulse voltammetry to achieve quantitative analysis. The sensor of this invention has a detection limit as low as 1.1 CFU / mL and a linear range of 4 × 10⁻⁶. 1 -4×10 8 The CFU / mL concentration exhibits good selectivity and stability in complex samples such as cerebrospinal fluid. The preparation method of this invention is simple, low-cost, and highly sensitive, and can be used for ultrasensitive detection of Listeria monocytogenes in fields such as central nervous system infections and food safety monitoring.

The application discloses an intracranial hematoma ultrasonic crushing device and relates to the field of medical equipment, which comprises an ultrasonic vibration rod, an adhesion device and a reflux pipe, the reflux pipe is arranged at the end of the adhesion device far from the ultrasonic vibration rod, and a reflux pipe inner cavity is arranged in the reflux pipe; a drainage pipe and a suction pipe are arranged on the rod end cover. The application can realize ultrasonic crushing and discharge of intracranial hematoma, is clean and complete, avoids residue, enables blood clots to be fully adsorbed and discharged, and enables the cerebrospinal fluid pressure in the cranial cavity to be normal and not to be blocked. The trace suction and supply type purifies the cerebrospinal fluid, does not affect the intracranial pressure, and avoids the phenomenon of unstable intracranial pressure.

Magnetic resonance imaging (MRI) is used to image the slow flow of cerebrospinal fluid (CSF) in subarachnoid and perivascular spaces, amongst other regions of the brain and central nervous system. The slow CSF flow imaging can be provided by using low velocity’ encoding (VENC) and an efficient data acquisition. Tailored image processing can be used to further improve the accuracy, specificity, and visualization of CSF flow dynamics.

Disclosed is an application of sBCMA as a biomarker in cerebrospinal fluid in diagnosis and monitoring of antibody-mediated central nervous system autoimmune diseases. Taking NMOSD as an example, the level of sBCMA in cerebrospinal fluid of NMOSD patients is significantly higher than that of control subjects matched for age and gender, and there is a positive correlation between the level of sBCMA in cerebrospinal fluid and a nerve injury-related index NFL, a blood-cerebrospinal fluid barrier breakdown-related index QAlb, or a neuroinflammation-related index sTREM2 in cerebrospinal fluid of NMOSD patients. This indicates that the level of sBCMA in cerebrospinal fluid can be used as a biomarker for the diagnosis of antibody-mediated central nervous system autoimmune diseases such as NMOSD and the evaluation of the severity of nerve injuries, the degree of damage to blood-cerebrospinal fluid barrier, and the level of neuroinflammation in patients.