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Urine sample combined markers for diagnosis of prostate cancer and detection kit and method

A detection kit and technology for prostate cancer, applied in analytical chemistry, clinical medicine and medical fields, can solve the problems of poor specificity and overdiagnosis of prostate cancer, and achieve the effect of high sensitivity and high specificity

Active Publication Date: 2018-07-31
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a new combination of small molecule metabolites for the early diagnosis of prostate cancer in view of the clinical problems that the specificity of early diagnosis of prostate cancer is not strong and overdiagnosis is easy to occur, and provide a combination marker that can be used to detect the above combination markers. way of things

Method used

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  • Urine sample combined markers for diagnosis of prostate cancer and detection kit and method
  • Urine sample combined markers for diagnosis of prostate cancer and detection kit and method
  • Urine sample combined markers for diagnosis of prostate cancer and detection kit and method

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Embodiment 1

[0018] 1. Urine Sample Collection

[0019] Before the collection of urine samples, all volunteers included in the study signed the informed consent.

[0020] Including 46 patients with prostate cancer and 20 patients with benign prostatic hyperplasia, all patients with prostate cancer were confirmed to be cancerous by clinical histopathology, and all patients with benign prostatic hyperplasia were confirmed to be non-cancerous by clinical histopathology. All the serum PSA concentrations were in the range of 4.0-10.0 ng / mL. All of them were fasting for more than 8 hours, and urine samples were collected the next morning, and the collected urine samples were stored in a -80 degree Celsius refrigerator for later use.

[0021] 2. Analysis method

[0022] 2.1 Urine sample pretreatment

[0023] Take out the urine sample from the -80°C refrigerator and thaw it in an ice bath, vortex to mix well, and quantitatively take 60 μL into a 1.5mL centrifuge tube. Add 240 μL of methanol ex...

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Abstract

The invention relates to a novel use of small molecule metabolites such as acetyl leucine, propionyl carnitine, butyryl carnitine, kynuric acid, xanthine nucleoside, 5-hydroxy-L-tryptophan, p-cresol sulfate and tryptophan as combined markers in a urine sample in preparation of a kit for diagnosing prostate cancer patients in subjects. The invention also relates to a kit for diagnosing prostate cancer patients in subjects. Through detection of the relative concentrations of the combined markers in urine samples of male subjects, based on a binary logistic regression equation, the combined marker variables and determined section values are calculated and it is determined that the subjects are prostate cancer patients or not. Through combination of the small molecule metabolites, the kit canbe used for auxiliary diagnosis of prostate cancer and especially for diagnosis of prostate cancer in the clinical diagnostic ash area.

Description

technical field [0001] The present invention relates to the application of a new small molecule combined marker in the diagnosis of prostate cancer. It belongs to the fields of analytical chemistry, clinical medicine and medicine. Background technique [0002] Prostate cancer (PCa) is one of the malignant tumors that seriously threaten men's health. Prostate cancer occurs mostly in developed countries in Europe and the United States. Its incidence rate ranks first among male malignant tumors, and its mortality rate ranks second, second only to lung cancer. In 2016, an estimated 180,890 new cases and 26,120 deaths from prostate cancer were estimated in the United States. The incidence of prostate cancer in my country is lower than that of developed countries in Europe and America, but it has gradually increased in recent years, and the age of onset tends to be younger. Due to the ever-changing pathological development process of prostate cancer, which varies greatly among ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/8818
Inventor 许国旺邵亚平赵欣捷
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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