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Device capable of detecting microorganism quickly

A microbial and rapid technology, applied in the field of microbial detection, can solve the problems of unfavorable climate and hot areas, such as transportation and storage, unfavorable detection of thermophilic bacteria, etc., to achieve reliable detection results, simple production, and low cost.

Active Publication Date: 2017-03-29
SHANGHAI MICROTREND BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the medium carrying the acid-base indicator described in it is mainly agar, and the agar is in a semi-solidified or flowing state at a temperature higher than 40°C, which is not conducive to transportation and storage in hot climate areas, and is also not conducive to the detection of thermophiles. bacteria

Method used

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  • Device capable of detecting microorganism quickly

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] like figure 1 As shown, a device for rapidly detecting microorganisms of the present invention includes a bottle cap 1 and a bottle body 2, and the bottle body 2 is divided into two parts, an upper layer and a lower layer: the upper layer is a culture area 21 containing a specific liquid culture medium; the lower layer is a The detection area 22 has a built-in sensor. The specific liquid culture medium, here specifically, may be nutrient broth, which is used to detect the total number of bacteria. The preparation process of the device for rapid detection of microorganisms is as follows:

[0020] 1. Prepare the sensor:

[0021] (1) Prepare color indicator solution: 0.5wt% bromothymol blue, 0.05wt% xylenol blue, dissolved in 20% ethanol solution;

[0022] (2) Mixing silicone material and color indicator solution: First, weigh 0.8g of linear methylhydrogen polysiloxane with a hydrogen content of 0.24%, 28.5g of α,ω-divinyl polydimethylsiloxane and Put 0.7g of 10ppm pla...

Embodiment 2

[0033] like figure 1 As shown, a device for rapidly detecting microorganisms of the present invention includes a bottle cap 1 and a bottle body 2, and the bottle body 2 is divided into two parts, an upper layer and a lower layer: the upper layer is a culture area 21 containing a specific liquid culture medium; the lower layer is a The detection area 22 has a built-in sensor. The specific liquid culture medium, specifically, may be wort juice liquid culture medium, which is used to detect mold and yeast. The preparation process of the device for rapid detection of microorganisms is as follows:

[0034] 1. Prepare the sensor:

[0035] (1) Preparation of color indicator solution: 0.2wt% bromothymol blue sodium salt, 0.3wt% phenol red sodium salt, dissolved in distilled water and heated to dissolve;

[0036] (2) Mix the silicone material and the color indicator solution: First, weigh 0.15g of linear methylhydrogenpolysiloxane with a hydrogen content of 0.8%, 29.7g of α,ω-diviny...

Embodiment 3

[0043] like figure 1 As shown, a device for rapidly detecting microorganisms of the present invention includes a bottle cap 1 and a bottle body 2, and the bottle body 2 is divided into two parts, an upper layer and a lower layer: the upper layer is a culture area 21 containing a specific liquid culture medium; the lower layer is a The detection area 22 has a built-in sensor. The specific liquid medium, here specifically, may be Brilliant Green Lactose Bile Salt Broth, which is used to detect coliform bacteria. The preparation process of the device for rapid detection of microorganisms is as follows:

[0044] 1. Prepare the sensor:

[0045] (1) Prepare color indicator solution: 0.1wt% bromothymol blue, 0.01wt% cresol red, dissolved in 20% ethanol solution;

[0046] (2) Mixing silicone material and color indicator solution: First, weigh 0.48g of cyclic methylhydrogen polysiloxane with a hydrogen content of 0.32%, 29.25g of methyl vinyl MQ silicone resin and 30ppm platinum-alk...

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Abstract

The invention discloses a device capable of detecting a microorganism quickly. The device utilizes the color-change principle of a color indicator to detect metabolites generated by the microorganism in the growing process so as to achieve microorganism detection. The device is a container capable of being sealed and divided into an upper layer and a lower layer; the upper layer is a cultivating area, a special fluid nutrient medium is arranged in the upper layer, and the fluid nutrient medium provides suitable nutritional conditions for growing of the target microorganism in a sample; and the lower later is a detecting area, a sensor which is made of organosilicon materials and a color indicator solution is arranged in the detecting area, and the sensor makes direct contact with the special fluid nutrient medium. When the target microorganism grows in the cultivating area, the micromolecule metabolites of the target microorganism permeates into the sensor, the color of the color indicator in the sensor is changed, and in this way, whether the target microorganism exists or not in the sample is judged. The device detects the microorganism by detecting the growing metabolites of the microorganism, detecting time can be greatly shortened, and the device has the advantages of being simple in manufacturing, low and cheap in cost, reliable in detecting results and the like.

Description

technical field [0001] The invention belongs to the field of microorganism detection, in particular to a device for rapidly detecting microorganisms. Background technique [0002] Microbial testing is a must-check item for all food, medicine, cosmetics, water and other products when they leave the factory, and it is also an important part of environmental monitoring. The current detection technology is mainly GB4789 series, most of which are plate method. Although the detection results are accurate, the detection process is cumbersome and time-consuming, and the detection results cannot be obtained in real time, which cannot meet the needs of enterprises for rapid release of products. Other detection technologies such as the disk method that coexist in the market save manpower, material resources and time for culture medium configuration and glass instrument cleaning, but the detection time of the whole microorganism has not been effectively shortened. The ATP bioluminesc...

Claims

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Application Information

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IPC IPC(8): C12M1/34
CPCC12M41/38
Inventor 袁莉红李芳彭晰钰
Owner SHANGHAI MICROTREND BIOTECH CO LTD
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