43results about How to "High diagnostic sensitivity" patented technology

A device and method of detection and treatment of a solid cancer tumor, for example prostate cancer. In a diagnostic method, ultrasound is used to extract a cancer tumor marker from interstitial fluid into a coupling medium. In a method for treating a solid tumor, ultrasound is used to deliver one or more drugs from the coupling medium directly to the site of the tumor.

An invention relating to therapeutic pharmacological agents and methods to chemically induce intracellular hyperthermia and/or free radicals for the diagnosis and treatment of infections, malignancy and other medical conditions. The invention relates to a process and composition for the diagnosis or killing of cancer cells and inactivation of susceptible bacterial, parasitic, fungal, and viral pathogens by chemically generating heat, and/or free radicals and/or hyperthermia-inducible immunogenic determinants by using mitochondrial uncoupling agents, especially 2,4 dinitrophenol and, their conjugates, either alone or in combination with other drugs, hormones, cytokines and radiation.

A method for diagnosing or differentially diagnosing a cancer characterized by the presence of cancer cells in the pleural fluid of a mammalian subject, the method comprising contacting a sample of pleural fluid of the subject with colloidal magnetic particles coupled to a ligand which binds to a determinant on a cancer cell, but does not bind above a baseline threshold to other cellular and non-cellular components in pleural fluid; subjecting the pleural fluid-magnetic particle mixture to a magnetic field to produce a cell fraction enriched in ligand coupled-magnetic particle-bound cancer cells, if present in the pleural fluid; and analyzing the enriched fraction for the number of cancer cells in the pleural fluid. In certain aspects, this method involves preparing the pleural fluids for the above-noted method steps by, e.g., dilution of unprocessed pleural fluid. In certain aspect, the pleural fluid is subjected to the diagnostic method within 24 hours of withdrawal from the subject. This method has advantages to present diagnostic procedures for identifying malignant pleural effusions. The tumor cells present in pleural fluid can be characterized with cellular and molecular markers to determine prognostic and predictive factors.

Disclosed herein are methods, reagents and apparatus for rapid detection of microorganisms in swab-collected samples collected from testing objects. Testing objects include food surface, and human body sites.

The invention discloses a double-fed wind generator rotor winding three-phase asymmetric load fault diagnosis method which includes the steps: 1 building a double-fed wind generator rotor winding fault simulation system on a dynamic simulation experiment platform; 2 acquiring rotor current or voltage signals on a rotor side before and after fault in different running states of a motor, correspondingly recording waves by a fault wave recording analysis device, reading data by a MATLAB (matrix laboratory), filtering direct current components and performing FFT (fast Fourier transform) processing; 3 extracting obviously changed frequency serving as fault diagnosis characteristic frequency before and after fault according to amplitude change to judge whether a double-fed wind generator rotor winding has three-phase asymmetric load faults or not. Diagnosis results are quite approximate to true values and effective, diagnosis accuracy is high, and operability is strong.

The present invention relates to: a composition for detecting a gastric cancer marker comprising a preparation for measuring the expression level or methylation level of mRNA of ADCY3 or a protein thereof; a kit for diagnosing gastric cancer, containing the composition; a method for diagnosing gastric cancer by treating a biological sample with the preparation to ascertain whether a material for complementarily binding to the preparation exists and comparing the amount thereof with a control group; a composition for treating and preventing gastric cancer, containing a preparation capable of inhibiting ADCY3 polynucleotide or ADCY3 polypeptide; and a method for treating gastric cancer by administering the composition to an individual. According to the present invention, it is possible to diagnose gastric cancer with high diagnostic sensitivity and specificity using ADCY3 as a marker for diagnosing gastric cancer, and thus can greatly contribute to reducing mortality due to gastric cancer.

The invention provides isolated, purified, recombinant receptor binding proteins Gp-6, Gp-10 and Gp-31 from mycobacterium phage L5, and methods for producing these recombinant receptor binding proteins. The invention also provides the use of recombinant receptor binding proteins, including Gp-6, Gp-10 and Gp-31, as probes for the identification and diagnosis of mycobacterium infections and conditions caused by mycobacterium. Further provided by the invention are methods of targeting mycobacteria using recombinant receptor binding proteins, including Gp-6, Gp-10 and Gp-31. Other methods provided by the invention include the use of recombinant receptor binding proteins, such as Gp-10, in diagnostic assays for diseases, such as Johne's disease, in animals, including ruminants.

The invention provides a qPCR method and a reagent for quickly detecting MRSA (Methicillin Resistant Staphylococcus Aureus). The qPCR method comprises the following steps: (1) preliminary preparationprocedure; (2) nucleic acid extraction and purification; (3) primer/probe design, synthesis and labeling; (4) qPCR amplification and result judgment; (5) detection lower limit and amplification linearrange test; (6) gene fragment sensitivity and specificity calculation; (7) diagnostic sensitivity and specificity calculation; (8) a statistical method. The qPCR method and the reagent provided by the invention have the benefits that the detection lower limit is as low as 4cfu/reaction, and the amplification linear range reaches 2.0*10<2-8> Copies/ml; the sensitivity and the specificity of MRSA identification are 100 percent (352/352) and are 100 percent (77/77) respectively, and the sensitivity and the specificity of MRSA detection are 100 percent (94/94) and 95.4 percent (94/98) respectively; the qPCR method is simple and convenient, quick, high in sensitivity and good in specificity and not only can improve the diagnostic ability of MRSA infection, but also can realize the quick detection, thereby gaining time for early and accurate treatment.

The invention provides an EB virus BFRF3-BZLF1 fusion protein, a gene, a vector containing the BZLF1 fusion protein, a host cell, a test strip, a production method and application thereof. The EB virus BFRF3-BZLF1 fusion protein is obtained by connecting the EB virus BFRF3 protein or an EB virus BFRF3 protein fragment with an EB virus BZLF1 protein or EB virus BZLF1 protein fragment through a connecting peptide, and preferably, the EB virus BFRF3-BZLF1 fusion protein has an amino acid sequence shown in SEQ ID NO:1. The EB virus BFRF3-BZLF1 fusion protein can be used as a target antigen for detecting an EB virus, provides a basis for screening and early diagnosing nasopharyngeal carcinoma, infectious mononucleosis and Burkitt lymphoma, and has the advantages of simplicity, rapidness, sensitivity and specificity.

The present invention relates to bio-secure means for the detection of pathogens and provides a bio-secure reaction vessel and methods of preparing a sample for PCR-based pathogen detection. The reaction vessel comprises a reaction chamber portion (1); a cap holder portion (2); and a cap (3). Two points of security, such as two O-ring seals (9, 10), are provided between the cap (3) and the reaction chamber portion (1). A first seal (9) may be located at a top of the cap holder portion (2) and a second seal (10) may be located at a base of the cap (10).

The invention provides a series of polypeptides obtained through screening with a phage display peptide library technology and having a prostate cancer targeting characteristic. The binding polypeptides have high specificity and can be applied to general survey of high risk groups of the prostate cancer, early clinical diagnosis of the prostate cancer and evaluation of a treatment effect.

The present invention relates to methods of screening biological samples for the presence of Strongyloides spp. More particularly, the present invention relates to a sensitive and specific screening test for the presence of anti-Strongyloides spp antibodies, protein or nucleic acid in subjects using particular Strongyloides spp L3 stage antigens and nucleic acids encoding same which have diagnostic efficacy.