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Composition, application and diagnostic kit

A composition and tissue technology, applied in the field of analytical chemistry, to achieve the effects of good diagnostic ability, high AUC, and good diagnostic accuracy

Active Publication Date: 2021-05-11
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This study found that the concentrations of glucose-6-phosphate, erythrose-4-phosphate, xanthine, and phosphatidylcholine PC (33:1) in the benign pulmonary nodules and the lesion tissues of lung cancer patients were significantly different. None of the four metabolites combined was reported for the diagnosis of lung cancer and benign pulmonary nodules

Method used

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  • Composition, application and diagnostic kit
  • Composition, application and diagnostic kit
  • Composition, application and diagnostic kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024]Tissue sample collection

[0025]Before sampling, all volunteers included in the study signed an informed consent.

[0026]The tumor lesion region tumor lesion zone in 85 patients with pulmonary tumors was collected under the same conditions, including 18 patients with lung-lung benign nodules and 47 patients with lung cancer, and 17 patients with lung cancer belong to early lung cancer patients. All patients have to be enema or taking a laxation before ridiculous tumor resection, for more than 8 hours before surgery, smokers quit smoking for 2-3 weeks before surgery. The tissue sample collected separately passed the gauze to take the blood, and then quickly transferred to the short-term preservation in liquid nitrogen, and finally transferred to the -80 ° C refrigerator for a long time.

[0027]2. Analytical method

[0028]2.1 Organizational samples pre-processed

[0029]The tissue samples were thawed on the ice at room temperature, and 10 mg tissue samples were weighed in 2 ml of centrifug...

Embodiment 2

[0053]In order to verify the combined markers, there is also a good diagnostic effect on patients with early lung cancer, and 20 patients samples samples were added. The analytical methods, instruments and reagents used in this experiment are completely consistent with Example 1.

[0054]1. Before organizing sample collection, the volunteer signed an informed consent.

[0055]Under the same conditions, 20 early lung cancer tissue samples were collected, stored, and preparation according to Example 1.

[0056]2. Organize testing and auxiliary diagnosis

[0057]Compared with the lung benign nodule tissue, glucose-6-phosphate and erythrose-4-phosphoric acid degraded significantly in early lung cancer, xanthine and phosphatidylcholine PC (33: 1) significantly increased in early lung cancer (seefigure 2 ). At the same time, the relative concentration of each marker in the early lung cancer tissue (the relative concentration of the same example in Example 1 indicates a metabolite concentration value ...

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Abstract

The invention relates to a novel application of small molecule metabolites glucose-6-phosphoric acid, erythritol 4-phosphoric acid, xanthine and phosphatidylcholine PC (33:1) in a tissue sample as a combined metabolic marker in preparation of a kit for diagnosing patients with lung cancer and lung benign nodules in subjects. The invention further relates to a kit for detecting the lung cancer and lung benign nodule patients in subjects. By detecting the relative concentrations of the combined markers in tissue samples of the subjects, the variable P value of the combined markers is calculated on the basis of a binary logistic regression equation, and then based on a determined cut-off value, the patients with lung cancer and lung benign nodules are diagnosed. In addition, the combined marker also has better diagnostic ability for patients with early lung cancer and lung benign nodules. The kit can realize high-sensitivity and high-efficiency detection of several small molecule metabolites involved in the invention, and has the characteristics of low detection cost and good repeatability.

Description

Technical field[0001]The present invention relates to the use of a new combined marker in the preparation of the kit for diagnosing lung cancer and lung cancer benign nodules. It belongs to the analysis of chemistry, biochemistry, and clinical medicine.Background technique[0002]Lung cancer is a malignant tumor with the highest mortality in global mortality. Early lung cancer is not obvious and interference between other lung benign nodules is a problem of clinical diagnosis of lung cancer. Although the study of lung cancer has always been a hot spot in oncology and clinical medicine, its pathogenic mechanism has not yet been clear, and it involves multi-gene genetic, environmental factors, and lifestyle. At present, serum tumor markers, imaging examination is the main method of pulmonary cancer screening. However, serum tumor marker cancer embryos have problems with low specificity and sensitivity in diagnosis of lung cancer. Imaging examination includes X-raindon slice, CT (compute...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574
CPCG01N33/57484G01N33/57423G01N2800/7066
Inventor 许国旺由蕾刘心昱
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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