Pleural fluid metabolite combination, kit and method for diagnosing tuberculous pleurisy

Abstract

The present invention relates to a pleural effusion metabolite combination, a kit and a method for diagnosing tuberculous pleurisy, the pleural effusion metabolite combination for diagnosing tuberculous pleurisy comprising dimethylglycine, tyrosine, kynurenine, and nicotinamide , betaine, acylcarnitine 12:0, acylcarnitine 14:2 and phosphatidylcholine 42:8. Kit for the diagnosis of tuberculous pleurisy, including for the detection of dimethylglycine, tyrosine, kynurenine, nicotinamide, betaine, acylcarnitine 12:0, acylcarnitine 14 in the extraction solution of pleural fluid samples :2 and Phosphatidylcholine 42:8 are the required internal standards. Through the combined markers involved in the present invention, the diagnosis of tuberculous pleurisy can be characterized with high sensitivity and high specificity. At the same time, the combined marker can be complementary to the traditional marker adenosine deaminase, and combined use can be used for auxiliary diagnosis of tuberculous pleurisy.

Description

technical field [0001] The invention relates to a diagnosis method for tuberculous pleurisy, in particular to a combination of pleural effusion metabolites, a kit and a method for diagnosing tuberculous pleurisy. Background technique [0002] Tuberculous pleurisy is a common clinical extrapulmonary tuberculosis with a relatively high incidence, accounting for about 44.1% of the total pleural diseases. In my country, the incidence of malignant pleurisy is as high as 900,000 per year, accounting for about 9% of the world's total. The morbidity and mortality rate of lung cancer is one of the highest tumors in the world, and about 40% of lung cancer patients will have malignant pleural effusion during the disease process. At present, due to the limitations of diagnostic techniques, the proportion of confirmed cases is very low. However, the prognosis and treatment of tuberculous pleurisy and malignant pleurisy are completely different. If not diagnosed and treated in time, it w...

AI Technical Summary

Problems solved by technology

[0003] At present, the methods for clinical diagnosis and identification of tuberculous pleurisy and malignant pleurisy include clinical manifestations, thoracentesis test results, pleural biopsy, bronchoscopy, surgical biopsy, etc. The most commonly used method is thoracentesis for cytological examination. Tumor cells found in pleural effusion have diagnostic value for malignant pleurisy, but due to the complex cell components in pleural effusion, it is sometimes difficult to distinguish tumor cells from inflammatory mesothelial cells, macrophages, etc. Influenced by other factors, its sensitivity is low (about 40%-60%), and the disadvantages of strong subjectivity are likely to cause missed diagnosis or misdiagnosis; while the diagnosis of tuberculous pleurisy mainly includes pleural effusion biochemical examination, tuberculin skin test ( TST), pleural effusion bacteriological examination (acid-fast stained smear and Mycobacterium tuberculosis culture), pleural histopathological biopsy, etc.

However, the specificity and sensitivity of the above-mentioned inspection methods are low, and some methods are invasive, high-risk, or have a long time period, which cannot meet the needs of clinical work. Therefore, the development of new and highly sensitive diagnostic techniques for pleurisy has very important clinical significance.

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