Small molecule metabolite map for identifying liver cancer, hepatitis or liver cirrhosis and manufacturing method thereof

A technology of small molecule metabolites and metabolites, which is applied in the field of hepatitis or liver cirrhosis small molecule metabolite map and its production, and the identification of liver cancer, which can solve the problems of early diagnosis of liver cancer that cannot be solved

Inactive Publication Date: 2012-05-09
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY +1
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Problems solved by technology

However, the current markers are still unable to solve the problem of early diagnosis of liver cancer, and so far there is no report on the use of blood small molecule metabolite profiles to identify liver cancer, hepatitis or cirrhosis

Method used

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  • Small molecule metabolite map for identifying liver cancer, hepatitis or liver cirrhosis and manufacturing method thereof
  • Small molecule metabolite map for identifying liver cancer, hepatitis or liver cirrhosis and manufacturing method thereof

Examples

Experimental program
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Embodiment Construction

[0023] Screening metabolite profiles for differential diagnosis of liver cancer from animal models.

[0024] 1. Animal model establishment and serum collection and processing

[0025] The research object is the SD (Sprague-Dawley) rat liver cancer model induced by diethylnitrosamine (DEN). The 6-week-old rats, weighing 120-150 grams, were purchased from the Shanghai Experimental Animal Center of the Chinese Academy of Sciences. They were raised in an SPF-grade environment, the light time was from 9:00 a.m. to 9:00 p.m., the temperature was kept at 22±2°C, and the relative humidity was 45%-60%. All animals were kept fed at random throughout the experiment. The model rats were divided into two groups, 10 of which were used to induce HCC. Diethylnitrosamine was administered by intragastric infusion at 70 mg / kg, administered once every other week for ten consecutive times. Another 10 rats were fed as a control group at the same time.

[0026] According to histopathological ex...

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Abstract

The invention relates to the technical field of medical diagnostics, in particular to a small molecule metabolite map for identifying liver cancer, hepatitis or liver cirrhosis and a manufacturing method thereof. The small molecule metabolites are four blood metabolites markers, i.e. palmitoyl hemolysis phosphoric acid ethanolamine glyceride, docosapentaenoic acyl hemolysis phosphatidyl choline, docosahexaenoic acyl hemolysis phosphatidyl choline and taurocholic acid, which respectively have the molecular weight of 453.2855, 569.3481, 567.3319 and 516.2916, and the corresponding ions of 454.2928, 570.3547, 568.3391 and 480.2776 detected through mass spectrum. Through experimentation on animals, the accuracy for determination of the liver cancer phase of a rat is 85 percent, and the accuracy for determination of the hepatitis or the liver cirrhosis is 94 percent. Clinical experiment results show that the accuracy for determination of the liver cancer is 85 percent, and the accuracy for determination of non-liver cancer is 71.9 percent. The method provided by the invention has the advantages of high sensitivity and high throughput, is superior to the existing liver cancer single diagnosis marker, and is applicable to screening and assisted diagnosis of the liver cancer.

Description

technical field [0001] The invention relates to the technical field of medical diagnostics, and relates to a small blood molecule metabolite map for identifying liver cancer, hepatitis or cirrhosis and a preparation method thereof. Background technique [0002] As an important technical means in the field of systems biology in the post-genome era, metabolomics has made great progress in recent years. Metabolomics focuses on the dynamic changes of all metabolites in cells, tissues, and organs after pathological stimulation or genetic modification, and systematically describes the occurrence and development of diseases through changes in metabolites from an overall perspective. All metabolites in body fluids can be used as indicators of the state of the living system, not only can reflect the results of various physiological adjustments, but also can be developed into a powerful tool for assisting clinical diagnosis. At present, corresponding diagnostic metabolic markers have...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/72
Inventor 王红阳许国旺谈冶雄尹佩源汤靓
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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