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Small molecule metabolite for improving effect of antibiotic to eliminate pathogenic bacteria

A technology of antibiotics and pathogenic bacteria, applied in the field of medicine

Active Publication Date: 2015-05-06
GUANGDONG LITAI PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on whether alanine can improve antibiotics and host clearance of pathogenic bacteria.

Method used

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  • Small molecule metabolite for improving effect of antibiotic to eliminate pathogenic bacteria
  • Small molecule metabolite for improving effect of antibiotic to eliminate pathogenic bacteria
  • Small molecule metabolite for improving effect of antibiotic to eliminate pathogenic bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1 The acquisition of Edwardsiella tarda artificial passage drug-resistant strains and the confirmation of wild drug-resistant bacteria

[0036] 1. Obtaining of Edwardsiella tarda LTB4 drug-resistant strain by artificial subculture: The minimum inhibitory concentration of Edwardsiella tarda LTB4 starting strain (named LTB4-S) to Kanamycin was detected by two-fold dilution method. Then use LTB4-S with 10 5 The number of colony-forming units / ml was continuously cultured for 10 generations in a medium containing 1 / 2 the minimum inhibitory concentration of kanamycin, and the minimum inhibitory concentration was determined. If its minimum inhibitory concentration / initial minimum inhibitory concentration > 4, the strain exhibits resistance to the antibiotic and is a drug-resistant strain. It was found that the minimum inhibitory concentration of the selected strain was 200 μg / mL, which was 64 times that of the starting strain LTB4-S (3.125 μg / mL), indicating that...

Embodiment 2

[0040] Embodiment 2 alanine can improve the susceptibility of tarda drug-resistant bacteria to kanamycin

[0041] 1. The susceptibility of drug-resistant bacteria to kanamycin increases with the increase of alanine concentration: add the final concentration of 40μg / mL (EIB202) or 800μg / mL (LTB4-R) to the prepared 5mL bacterial liquid At the same time as namycin, add alanine at different concentrations without or with different concentrations, so that the final concentrations of alanine are 0, 5, 10, 20, 40, and 80 mM respectively; culture at 30°C and 200rpm for 6 hours, and detect with a plate The number of viable bacteria was calculated to calculate the survival rate under different metabolite concentrations. The calculation formula is: survival rate (%) = (the number of viable bacteria after adding alanine / the number of viable bacteria without adding alanine) × 100%, see the results figure 1 a. Depend on figure 1 A shows that even if the lowest concentration of alanine ...

Embodiment 3

[0044] Example 3 Alanine increases the susceptibility of drug-resistant bacteria to kanamycin and is universal

[0045] 1. Screening and identification of other antibiotic-resistant strains of Edwardsiella tarda: the minimum resistance to ampicillin, baloxacin and tetracycline of Edwardsiella tarda LTB-4 starting strain (named LTB-S) was detected by two-fold dilution method. Inhibitory concentration. Convert LTB-S to 10 5 The number of colony-forming units / ml was continuously cultured for 10 generations in a medium containing 1 / 2 the minimum inhibitory concentration of ampicillin or baloxacin or tetracycline, and the minimum inhibitory concentration was re-determined. If the re-measured minimum inhibitory concentration / initial minimum inhibitory concentration >4, the strain is considered to be resistant to the antibiotic. Depend on figure 2 The results of the determination showed that the ampicillin, baloxacin and tetracycline-resistant strains were screened.

[0...

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PUM

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Abstract

The invention belongs to the field of biological medicines, and discloses a novel use of a small molecule compound alanine. The research shows that the alanine is capable of enhancing the effect of the antibiotic to kill pathogenic bacteria including drug-resistance bacteria. The small molecule metabolite alanine provided by the invention can be used as a medicine for improving the sterilizing function of the antibiotic.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a small molecular metabolite that can improve the elimination of pathogenic bacteria by antibiotics. Background technique [0002] Pathogenic bacteria seriously endanger human health, and it is necessary to take effective measures to prevent and control them. Although antibiotics can effectively kill bacteria, due to the misuse of antibiotics, bacteria have developed drug resistance. Antibiotic-resistant bacteria reduce or eliminate the effectiveness of otherwise effective antibiotic treatment, making infections difficult to control. Therefore, it is very important to adopt new methods to control the infection of bacteria, especially drug-resistant bacteria. [0003] Enhancing the susceptibility of bacteria to antimicrobials is a hot research field in the control of multi-drug resistant bacteria. This antibacterial pathway needs to be established on the basis of ...

Claims

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Application Information

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IPC IPC(8): A61K31/7036A61K31/7004A61K31/546A61K31/4709A61K31/427A61K31/7048A61P31/04A61K31/198
Inventor 彭宣宪李惠苏玉斌
Owner GUANGDONG LITAI PHARM CO LTD
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