Manufacturing and diagnosis application of blood micro-molecular metabolin specific chromatogram

Abstract

The invention relates to the technical field of medical diagnostics. Thyroid tumors can be divided into two types including benign tumors and cancers. The property of a thyroid nodule is difficult to determine clinically; and even if biopsy is carried out, the benign tumors and the cancers are not easy to clearly identify due to thyroid adenomas and focal nodular hyperplasia. The invention provides a blood micro-molecular metabolin specific chromatogram which is used for identifying papillary thyroid carcinomas, benign thyroid tumors and blood of normal people, and a manufacturing method of the blood micro-molecular metabolin specific chromatogram. A micro-molecular metabolin comprises two blood metabolin marks, namely palmityl carnitine and carnitine; the molecular weights of the palmityl carnitine and the carnitine are 400.2634 and 161.20; and corresponding ions detected on a mass spectrum are 404.5532 and 160.7866. A clinical experiment shows that the accuracy of applying a chromatogram formed by a specific value of the two metabolism micro-molecules to judge the thyroid cancer is 74% and the accuracy of judging non-cancer (the benign thyroid tumor and the normal people) is 72.3%. The blood micro-molecular metabolin specific chromatogram disclosed by the invention has the advantage of high sensitivity and can be prepared into a diagnosis kit for screening and auxiliary diagnosis of the thyroid cancer.

Description

technical field [0001] The invention relates to the technical field of medical diagnostics, in particular to a blood small-molecule metabolite map for differentiating papillary thyroid cancer, benign goiter and normal people, a production method and application thereof. Background technique [0002] As an important technical means in the field of systems biology in the post-genome era, metabolomics has made great progress in recent years. Metabolomics focuses on the dynamic changes of all metabolites in cells, tissues, and organs after pathological stimulation or genetic modification, and systematically describes the occurrence and development of diseases through changes in metabolites from an overall perspective. All metabolites in body fluids can be used as indicators of the state of the living system, not only can reflect the results of various physiological adjustments, but also can be developed into a powerful tool for assisting clinical diagnosis. At present, correspo...

AI Technical Summary

Problems solved by technology

Clinically, it is difficult to determine the nature of thyroid nodules. Even with pathological biopsy, sometimes thyroid adenomas and nodular hyperplasia, benign tumors and malignant tumors are not easy to clearly identify

[0006] At present, there are still no markers that can be used to judge benign and malignant thyroid nodules, and there are no related reports on the use of blood small molecule metabolite profiles to identify papillary thyroid cancer, benign thyroid nodules, or normal people

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