Primers and method for detecting and typing human papilloma viruses in esophagi

A technology of HPV virus and esophagus, which is applied in the field of primers and methods for detecting and typing esophageal HPV virus, can solve problems such as application limitations, achieve the effect of reducing the use of consumables, simple and stable reagent consumables, and eliminating false positives

Active Publication Date: 2011-01-12
BGI GENOMICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this method is currently mainly used to detect and type HPV that infects cervical epithelial cells, and its application is limited.

Method used

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  • Primers and method for detecting and typing human papilloma viruses in esophagi
  • Primers and method for detecting and typing human papilloma viruses in esophagi
  • Primers and method for detecting and typing human papilloma viruses in esophagi

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Embodiment Construction

[0044] The embodiment of the present invention provides a method for detecting and typing the above 15 HPV types that infect esophageal cells, which includes the following steps.

[0045] (1) According to the gene sequences of the selected HPV types to be detected (that is, the above-mentioned 15 HPV types), on the basis of GP5+ / GP6+ universal primers, design amplification primers specific to each type, so The amplification primer has 12 bases that completely match the gene sequence of the type it is targeting at the 3' end, and has a tag sequence of 10 bases (acgttggatg) at the 5' end; the extension primer is designed, and the extension primer The length is 17-28 bases, and contains a type-specific polymorphic site marker in the 4 bases at its 3' end including the extended base, and the position selected for the extended primer is GP5+ / A relatively conserved region in the GP6+ sequence; wherein, the molecular weight difference between the extension product and the extension ...

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Abstract

The invention relates to primers and a method for detecting and typing human papilloma viruses (HPV) infecting esophageal cells (such as esophageal mucosa cells). In particular, the invention designs a set of brand-new primers (including amplification primers and extension primers) based on the mass spectrometry detection technology of a matrix assisted laser desorption / ionization time-of-flight mass spectrometry system (MALDI-TOF-MS), thus realizing detection and typing of 15 types infecting the esophageal cells.

Description

field of invention [0001] The present invention relates to primers and methods for detecting and typing human papillomaviruses that infect esophageal cells (eg, esophageal mucosal cells). Background of the invention [0002] Human papilloma virus (HPV) is a small, non-enveloped DNA virus with a double-stranded closed-circle DNA genome, with a size of about 7.2-8kb and a relative molecular weight of about 5×10 6 . The HPV genome can be divided into 3 regions: the non-coding upstream regulatory region; the early open reading frame region, including E6, E7, E1, E2, E4, E5; the late open reading frame region, encoding the major capsid protein L1 and the small coat Capsid protein L2. According to the difference of the open reading frame (ORF) gene sequence encoding the main capsid protein L1, HPVs can be typed: the HPVs whose ORF similarity in the L1 region is less than 60% are divided into different genera, and the similarity between 60- HPVs with a similarity between 70% and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/70C12Q1/68G01N27/62G01N27/626
CPCC12Q2531/113C12Q1/708
Inventor 倪培相瞿宁李晓艳
Owner BGI GENOMICS CO LTD
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