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32results about How to "Broad economy" patented technology

High metastatic potential hepatoma cell line capable of steady autophagy indication, and establishment method and application method thereof

InactiveCN102115730AHigh metastatic potentialDynamic observation of autophagy changesMicrobiological testing/measurementMicroorganism based processesMorphological filterIn vivo
The invention belongs to the field of biomedical science, and aims to provide a high metastatic potential hepatoma cell line capable of steady autophagy indication, and an establishment method and an application method thereof. Lentivirus expression vectors containing EGFP (enhanced green fluorescent protein)-LC3 (light chain 3) autophagy reporter genes are constructed and used to infect hepatoma cells with high metastatic potential, so that host cells can express the EGFP-LC3 steadily and efficiently and indicate autophagy changes of the hepatoma cells steadily. To apply the cell line, metastasis research models can be established in vitro and in vivo for the observation on autophagy changes during metastasis, and the cell autophagy changes can be analyzed rapidly and quantitatively by adopting an image analysis method based on Top-hat operators and morphological filter. The high metastatic potential hepatoma cell line has the characteristics that autophagy indication is conducted steadily, reliably and sensitively in a real-time manner, the metastasis occurs definitely, the autophagy is quantified rapidly and accurately, the operation is simple and convenient, and the like, and can greatly facilitate correlation study on autophagy and metastasis.
Owner:ZHONGSHAN HOSPITAL FUDAN UNIV

Normal-low pressure high-low temperature human body thermal comfort experiment environment cabin

The invention, which belongs to the field of building heating-ventilation air conditioners, relates to a normal-low-pressure high-low-temperature human body thermal comfort experiment environment cabin comprising an environment cabin body, a pressure control system, a temperature and humidity control system, a fresh air control system, an oxygen concentration control system, an illumination control system, an environment monitoring and controlling system and a human body physiological parameter measuring system. The pressure control system, the temperature and humidity control system, the fresh air control system, and the oxygen concentration control system are arranged at the outer side of the environment cabin body; and the illumination control system, the environment monitoring and controlling system and the human body physiological parameter measuring system are arranged in the environment cabin body. The pressure control system is mainly formed by sequentially connecting a vacuumpump and a vacuum tank with the environment cabin body. The temperature and humidity control system is mainly formed by sequentially connecting a direct evaporation surface cooling section, an electric heating section, a fan section and a humidifying section with the environment cabin body. The fresh air control system is mainly formed by sequentially connecting a filter, a dehumidifier, a gas-gasheat exchanger and a silencer with the environment cabin body.
Owner:INST OF DEFENSE ENG ACADEMY OF MILITARY SCI PLA CHINA

S-type pitot tube coefficient calibration method based on CFD numerical calculation

The invention discloses an S-type pitot tube coefficient calibration method based on CFD numerical calculation. The S-type pitot tube coefficient calibration method comprises the steps of constructionof a calibrated S-type pitot tube and a three-dimensional geometric model of a calibration wind tunnel, generation of a calculation grid, solving of a Fluent solver, result post-processing and S-typepitot tube coefficient calculation. According to the invention, a CFD numerical simulation method is adopted to fully consider the influence of the addition of the S-type pitot tube on the flow fieldin the wind tunnel; the complexity of a flow field around the S-shaped pitot tube is considered; the problem of high investment in the original S-shaped pitot tube real-flow test is solved; and the efficiency is low, the real flow mark timing is accurately reproduced; the flow field in the whole wind tunnel and the surrounding flow field form of the S-shaped pitot tube are formed; Therefore, theaccurate calculation of the S-type pitot tube coefficient is realized, a simple, efficient and low-price method is provided for the calibration of the S-type pitot tube in the future, the design costof the new S-type pitot tube and the calibration cost of the S-type pitot tube coefficient in use are greatly reduced, and the application prospect and the economic value are wide.
Owner:SHANGHAI INST OF MEASUREMENT & TESTING TECH

Yak meat tenderizing and color-improving technology

The invention discloses a yak meat tenderizing and color-improving technology. The yak meat tenderizing and color-improving technology comprises the following steps: (1) shaping yak meat chunks, namely shaping acid-excreted mature yak meat chucks, and removing connective tissues such as sinews and tendons; (2) injecting tenderizing liquid, namely preparing tenderizing liquid, and uniformly injecting the tenderizing liquid into the yak meat chunks to be treated by using rowed syringe needles; (3) carrying out rolling-massage, namely performing rolling-massage on the yak meat chunks injected with the tenderizing liquid in a rolling machine so as to have the fiber of the yak meat infiltrated with the tenderizing liquid, and carrying out full reaction; (4) carrying out cutting and packaging, namely cutting the yak meat chunks after rolling-massage into pieces, and carrying out vacuum packaging; and (5) carrying out liquid high-hydrostatic-pressure treatment, namely performing liquid high-hydrostatic-pressure treatment on the vacuum-packaged yak meat so as to improve meat texture, and completing the tenderizing and color-improving processes. The yak meat tenderizing and color-improvingtechnology is capable of achieving the goal of tenderizing yak meat under the premise of preserving original color and luster of the yak meat; moreover, the yak meat tenderizing and color-improving technology is simple in processes, obvious in effects, low in processing cost, and significant in economic benefits.
Owner:XIHUA UNIV

Endurance test failure judgment method for automobile transmission system

The invention provides an endurance test failure judgment method for an automobile transmission system. The endurance test failure judgment method includes the steps that i different working condition points t are set according to different rotating speeds n and different torques M, testing is carried out on a traditional system test piece at each working condition point t, the transmission efficiency at each working condition point t is obtained, then the i working condition points t are repeatedly tested, the transmission efficiency is calculated, an efficiency matrix is formed, and data filtering is carried out on the efficiency matrix; the mean values (please see the specification) and the variances sigma of the transmission efficiency of the working condition points t in the efficiency matrix are calculated, and a mean value change curve and a variance change curve are drawn; a mean value threshold value and a variance threshold value are set, when the mean values (please see the specification) are lower than the set mean value threshold value or the variances sigma are larger than the set variance threshold value, an alarm signal is emitted out, a device is notified to take stopping measures, and it is judged that the test piece fails. By means of the endurance test failure judgment method, interference can be effectively reduced, the foreboding generated before the transmission system fails can be effectively found, and early diagnosis can be achieved.
Owner:CHINA AUTOMOTIVE ENG RES INST +1

Integrated transfer learning classification method and system for single-view full-polarization SAR data

The invention provides an integrated transfer learning classification method for single-view fully polarized SAR data, and the method comprises the steps of employing full-image segmentation and local windowing segmentation, and employing the context information of an image space to concentrate the labeled sample size of a source domain and expand the labeled sample size of a target domain; then, setting a source domain labeled sample selection process by combining a scattering vector similarity coefficient and a fitness function, and adjusting source domain sample distribution used by a training evaluator model according to a target domain category center; introducing reference information of the reliability of the weak classifier as a weight factor, performing category soft prediction by using the migration weak classifier in a weighting form, and giving a target domain unlabeled pixel false label with a consistent prediction result to expand the labeled sample size of the target domain and train a new weak classifier; and completing ensemble classification of all pixels in the target domain by iteratively utilizing the generated migration weak classifier set. The invention can significantly reduce the high dependence of a radar image classification task on target domain label information, and improves the classification efficiency and the automation level.
Owner:WUHAN UNIV

Method for extracting small-molecular oyster polypeptides

The invention discloses a method for extracting small-molecular oyster polypeptides; biological enzymatic hydrolysis and membrane technique are scientifically and reasonably integrated, the raw material is biologically enzymatically hydrolyzed through a specific composite enzyme, activated carbon may remove color and fishy smell and remove heavy metals and harmful substances, separation, concentration and purification are performed successively with an ultrafiltration membrane and a nanofiltration membrane having specific molecular weight cut-offs to obtain concentrate with small-molecular oyster polypeptides, and the concentrate is spray-dried to obtain the finished small-molecular oyster polypeptide; the method is highly operable, has an extraction rate of 0.8-3.0% for small-molecular oyster polypeptides, the prepared small-molecular oyster polypeptides are high in purity, the bioactivity of the small-molecular oyster polypeptides can be effectively guaranteed, the small-molecular oyster polypeptides belong to third-generation functional food made with oyster and may then act as a material for various high-added-value products, such as various functional peptide effervescent tablets, health products, nutrients and pharmaceuticals, and the richer forms of oyster downstream products are provided.
Owner:冯群力 +2

Extracting method of small-molecular sea cucumber-oyster polypeptide

The invention discloses an extracting method of a small-molecular sea cucumber-oyster polypeptide. A biological enzymolysis technology and a membrane technology are scientifically and reasonably integrated, and raw materials are subjected to biological enzymolysis through specific compound enzyme; active carbon can have the effects of removing color and fishiness and removing heavy metal and harmful substances; a concentrated solution containing the small-molecular sea cucumber-oyster polypeptide is obtained by sequentially separating, concentrating and purifying through an ultra-filtration membrane and a nano-filtration membrane, which have specific molecular weight cutoff; and finally, the concentrated solution is sprayed and dried to obtain a small-molecular sea cucumber and oyster polypeptide finished product. The method disclosed by the invention has high operability and the extracting rate on the small-molecular sea cucumber-oyster polypeptide is 1.0%-3.8%; the prepared small-molecular sea cucumber-oyster polypeptide has high purity and the bioactivity of the small-molecular sea cucumber-oyster polypeptide can be effectively guaranteed; and the small-molecular sea cucumber-oyster polypeptide cab be used as the raw material of various high-additional-value products in future, and has a wide market space and economic value.
Owner:冯群力 +2

Constant-temperature and constant-humidity unfreezing device based on Internet of Things and control method thereof

InactiveCN111919880AUnfreeze GuaranteeGood defrosting qualityTransmission systemsMeat/fish preservation by freezing/coolingThe InternetHeat conservation
The invention relates to a constant-temperature and constant-humidity unfreezing device based on the Internet of Things and a control method thereof. The unfreezing device comprises a heat preservation and insulation warehouse body, an electric heating system, a humidifying system, a refrigerating system, a control system and a remote control system. The device comprises two working modes, namelyan unfreezing mode and a fresh-keeping mode. In the unfreezing mode, the electric heating system and the refrigerating system are jointly controlled to achieve variable-temperature unfreezing control,the humidifying system conducts humidity control according to the set target humidity, and it is guaranteed that the unfreezing process is conducted in the constant high-humidity environment. And inthe fresh-keeping mode, the refrigerating system performs constant-temperature control. Corresponding food thawing and fresh-keeping treatment can be carried out according to different requirements, remote online parameter configuration and control can be realized, thawing parameters are greatly convenient to set and update, the quality of thawed food is guaranteed, and the economic benefit is considerable.
Owner:NANKAI UNIV
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