Method for extracting small-molecular oyster polypeptides

A technology of oyster polypeptide and extraction method, which is applied in the field of extraction of small molecule oyster polypeptide, to achieve the effects of alleviating oversupply, ensuring biological activity, broad market space and economic value

Inactive Publication Date: 2017-03-08
冯群力 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The application of macroporous adsorption resin for industrial extraction and separation of oyster functional peptides is still blank in terms of technology and application

Method used

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  • Method for extracting small-molecular oyster polypeptides
  • Method for extracting small-molecular oyster polypeptides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The method for extracting small molecule oyster polypeptides in Example 1 includes the following steps:

[0017] (1) Using fresh oyster meat that has been desilted and washed as raw material, put the raw material and water into the biological enzymatic hydrolysis tank at a volume ratio of 1:3, and add compound enzymes and activated carbon to the biological enzymatic hydrolysis tank. The compound enzyme performs compound enzymatic hydrolysis treatment on the raw materials, in which the addition amount of activated carbon is 3% of the weight of oyster meat, the addition amount of compound enzyme is 0.5% of the weight of oyster meat, and the compound enzyme is composed of collagenase and alkaline protease at a ratio of 1:1 The compound enzymatic hydrolysis treatment conditions are as follows: the reaction temperature is 55°C, the pH value is 6.5, the reaction time is 12 hours, and the enzymatic hydrolysis product is obtained after the compound enzymatic hydrolysis treatment;

...

Embodiment 2

[0019] The method for extracting small molecule oyster polypeptides in Example 2 includes the following steps:

[0020] (1) Using fresh oyster meat that has been desilted and washed as raw material, put the raw material and water into a biological enzymatic hydrolysis tank at a volume ratio of 1:6, and add compound enzymes and activated carbon to the biological enzymatic hydrolysis tank. The compound enzyme performs compound enzymatic hydrolysis treatment on the raw materials. Among them, the amount of activated carbon added is 2% of the weight of oyster meat, the amount of compound enzyme added is 1.9% of the weight of oyster meat, and the compound enzyme is composed of collagenase and alkaline protease at a ratio of 2:3 The compound enzymatic hydrolysis treatment conditions are as follows: the reaction temperature is 50°C, the pH value is 7.0, the reaction time is 7 hours, and the enzymatic hydrolysis product is obtained after the compound enzymatic hydrolysis treatment;

[0021]...

Embodiment 3

[0022] The method for extracting small molecule oyster polypeptides in Example 3 includes the following steps:

[0023] (1) Using fresh oyster meat that has been desilted and washed as raw material, put the raw material and water into the biological enzymatic hydrolysis tank at a volume ratio of 1:2, and add compound enzymes and activated carbon to the biological enzymatic hydrolysis tank. The compound enzyme performs compound enzymatic hydrolysis treatment on the raw materials. The addition amount of activated carbon is 5% of the weight of oyster meat, the addition amount of compound enzyme is 0.3% of the weight of oyster meat, and the compound enzyme is composed of collagenase and alkaline protease at a ratio of 2:1 The compound enzymolysis treatment conditions are as follows: the reaction temperature is 60°C, the pH value is 6.0, the reaction time is 16 hours, and the enzymolysis product is obtained after the compound enzymolysis treatment;

[0024] (2) Pour the enzymatic hydrol...

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Abstract

The invention discloses a method for extracting small-molecular oyster polypeptides; biological enzymatic hydrolysis and membrane technique are scientifically and reasonably integrated, the raw material is biologically enzymatically hydrolyzed through a specific composite enzyme, activated carbon may remove color and fishy smell and remove heavy metals and harmful substances, separation, concentration and purification are performed successively with an ultrafiltration membrane and a nanofiltration membrane having specific molecular weight cut-offs to obtain concentrate with small-molecular oyster polypeptides, and the concentrate is spray-dried to obtain the finished small-molecular oyster polypeptide; the method is highly operable, has an extraction rate of 0.8-3.0% for small-molecular oyster polypeptides, the prepared small-molecular oyster polypeptides are high in purity, the bioactivity of the small-molecular oyster polypeptides can be effectively guaranteed, the small-molecular oyster polypeptides belong to third-generation functional food made with oyster and may then act as a material for various high-added-value products, such as various functional peptide effervescent tablets, health products, nutrients and pharmaceuticals, and the richer forms of oyster downstream products are provided.

Description

Technical field [0001] The invention relates to a method for extracting small molecule polypeptides in seafood, in particular to a method for extracting small molecule oyster polypeptides. Background technique [0002] There are many kinds of creatures in the ocean, and the resources are extremely rich, and the biological chain is basically sound, and the regeneration ability is strong. Marine organisms are rich in proteins, unsaturated fatty acids, and taurine that are beneficial to human health. Their trace element spectrum is also much better than terrestrial organisms, and they are an excellent source of human health food. The marine environment is very different from the terrestrial environment, such as high pressure, low temperature, high temperature (submarine volcanic craters and nearby), and high salinity. In order to be suitable for the marine environment, marine biological proteins are very different from terrestrial biological proteins in terms of amino acid composit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34
CPCC12P21/06C07K1/34
Inventor 冯群力张建芳冯志江冯瑜张夏绒
Owner 冯群力
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