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Extracting method of small-molecular sea cucumber-oyster polypeptide

An extraction method and small molecule technology, applied in the field of extraction of small molecule ginseng and oyster polypeptides, can solve problems such as technical and application gaps, achieve broad market space and economic value, ensure biological activity, and have the effects of strong operability.

Inactive Publication Date: 2017-05-10
冯群力 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few high-value-added products using sea cucumber and oyster as raw materials, and the industry has not yet developed an effective method for the extraction of small-molecule ginseng-oyster peptides (that is, peptides composed of small-molecule sea cucumber peptides and small-molecule oyster peptides) , the industrial extraction of small molecule ginseng oyster peptides is still blank in terms of technology and application

Method used

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  • Extracting method of small-molecular sea cucumber-oyster polypeptide
  • Extracting method of small-molecular sea cucumber-oyster polypeptide

Examples

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Embodiment 1

[0021] The extraction method of the small molecule ginseng oyster polypeptide of embodiment 1 comprises the following steps:

[0022] (1) Cleaning: take fresh sea cucumbers and oysters as raw materials, wash the surface silt of sea cucumbers and oysters with clear water;

[0023] (2) Shelling oysters: In an environment with a temperature below 5°C, use a special oyster knife to take out the oyster meat for later use, and store it for no more than 24 hours;

[0024] (3) Pulverization: Drain the oyster meat moisture until no water drips, and pulverize the oyster meat and sea cucumber with an industrial crusher, and pulverize them into particles less than 2 cm;

[0025] (4) Enzymolysis: add crushed oyster meat and sea cucumber tap water or purified water to the biological enzymatic hydrolysis tank at a weight ratio of 1:2, and add alkaline protease, collagenase and papaya to the biological enzymatic hydrolysis tank Protease and 200 mesh gacs, the addition of alkaline protease is...

Embodiment 2

[0030] The extraction method of the small molecule ginseng oyster polypeptide of embodiment 2 comprises the following steps:

[0031] (1) Cleaning: take fresh sea cucumbers and oysters as raw materials, wash the surface silt of sea cucumbers and oysters with clear water;

[0032](2) Shelling oysters: In an environment with a temperature below 5°C, use a special oyster knife to take out the oyster meat for later use, and store it for no more than 24 hours;

[0033] (3) Pulverization: Drain the oyster meat moisture until no water drips, and pulverize the oyster meat and sea cucumber with an industrial crusher, and pulverize them into particles less than 2 cm;

[0034] (4) Enzymolysis: add crushed oyster meat and sea cucumber tap water or purified water to the biological enzymatic hydrolysis tank at a weight ratio of 1:5, and add alkaline protease, collagenase and papaya to the biological enzymatic hydrolysis tank Protease and 200 mesh gacs, the addition of alkaline protease is ...

Embodiment 3

[0039] The extraction method of the small molecule ginseng oyster polypeptide of embodiment 3 comprises the following steps:

[0040] (1) Cleaning: take fresh sea cucumbers and oysters as raw materials, wash the surface silt of sea cucumbers and oysters with clear water;

[0041] (2) Shelling oysters: In an environment with a temperature below 5°C, use a special oyster knife to take out the oyster meat for later use, and store it for no more than 24 hours;

[0042] (3) Pulverization: Drain the oyster meat moisture until no water drips, and pulverize the oyster meat and sea cucumber with an industrial crusher, and pulverize them into particles less than 2 cm;

[0043] (4) Enzymolysis: add crushed oyster meat and sea cucumber tap water or purified water to the biological enzymatic hydrolysis tank at a weight ratio of 1:7, and add alkaline protease, collagenase and papaya to the biological enzymatic hydrolysis tank Protease and 200 mesh gacs, the addition of alkaline protease is...

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Abstract

The invention discloses an extracting method of a small-molecular sea cucumber-oyster polypeptide. A biological enzymolysis technology and a membrane technology are scientifically and reasonably integrated, and raw materials are subjected to biological enzymolysis through specific compound enzyme; active carbon can have the effects of removing color and fishiness and removing heavy metal and harmful substances; a concentrated solution containing the small-molecular sea cucumber-oyster polypeptide is obtained by sequentially separating, concentrating and purifying through an ultra-filtration membrane and a nano-filtration membrane, which have specific molecular weight cutoff; and finally, the concentrated solution is sprayed and dried to obtain a small-molecular sea cucumber and oyster polypeptide finished product. The method disclosed by the invention has high operability and the extracting rate on the small-molecular sea cucumber-oyster polypeptide is 1.0%-3.8%; the prepared small-molecular sea cucumber-oyster polypeptide has high purity and the bioactivity of the small-molecular sea cucumber-oyster polypeptide can be effectively guaranteed; and the small-molecular sea cucumber-oyster polypeptide cab be used as the raw material of various high-additional-value products in future, and has a wide market space and economic value.

Description

technical field [0001] The invention relates to a method for extracting small-molecule polypeptides from seafood, in particular to a method for extracting small-molecule ginseng polypeptides. Background technique [0002] With the rapid development of science and technology and the popularization of nutritional knowledge, as well as the increase of per capita income and consumption level, human beings' requirements for food have transitioned from food and clothing to nutritional level. Natural, healthy, nutritious and safe have become the food industry in the 21st century. major issues of development. Collagen, as an important biological tissue structural protein polypeptide, has attracted more and more people's attention and interest, with huge market potential and high economic value. Compared with macromolecular proteins, collagen small molecule active peptides not only have the characteristics of low synthesis cost, high utilization rate, low toxicity and immunogenicity...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34
CPCC12P21/06C07K1/34
Inventor 冯群力张建芳冯志江冯瑜张夏绒
Owner 冯群力
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