Lung-targeting metastatic human hepatoma cell strain and establishing method thereof

A technology of human liver cancer cell line and liver cancer cell line, which is applied in the field of human liver cancer cell line HCCLM3-R-LM1 and its establishment, can solve the problem of no lung-targeted metastatic human liver cancer cell line and achieve delayed fluorescence quenching Effects of time, increased fluorescence expression intensity, and strong transfer ability

Inactive Publication Date: 2010-09-15
ZHONGSHAN HOSPITAL FUDAN UNIV
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Problems solved by technology

[0004] There is no report on the lung-targeted metastatic human liver cancer cell line that can stably express fluorescence in domestic and foreign literature

Method used

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  • Lung-targeting metastatic human hepatoma cell strain and establishing method thereof
  • Lung-targeting metastatic human hepatoma cell strain and establishing method thereof
  • Lung-targeting metastatic human hepatoma cell strain and establishing method thereof

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Embodiment 1

[0024] 1. Establishment of HCCLM3R nude mouse subcutaneous xenograft tumor model: BALB / c nu / nu male nude mice, 5-6 weeks old, weighing 15-18 grams, were raised in the nude mouse room of our institute without specific pathogens. Inject HCCLM3R, a highly metastatic fluorescent cell of liver cancer cultured in vitro, subcutaneously on the left side of the back, at a dose of 2X10 7 Cells / only, subcutaneous tumors were aseptically harvested after 4 weeks of growth, cut into 1mm 3 The left and right tumor pieces were used for follow-up experiments.

[0025] 2. Establish HCCLM3R nude mouse liver transplantation tumor model: locate the nude mouse liver, disinfect the skin outside the liver, incise the skin aseptically to expose the mouse liver, separate the liver capsule with tweezers and make a small opening, and implant the tumor piece into the liver On the surface, the liver capsule and skin were sutured.

[0026] 3. Acquisition of lung metastatic liver cancer cells: the orthotop...

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Abstract

The invention provides a lung-targeting metastatic human hepatoma cell strain and an establishing method thereof. The lung-targeting metastatic human hepatoma cell strain is characterized in that the mother cell is a red fluorescent protein expression hepatoma cell HCCLM3-R; the cell is named as a lung-targeting metastatic human hepatoma cell strain HCCLM3-R-LM1 by classifying, has the preservation number of 3644, and has the following biological characteristics that the cell is in polygonal epithelium shape, grows clinging to the wall, loses contact growth inhibition, has a hypo-triploid caryogram with the chromosome number of 50-58, secretes AFP protein, and has lung-targeting metastatic clonal selection advantage. The establishing method comprises the following steps of: sieving by adopting nude mouse subcutis and liver orthotopic implantation tumour model autogenetic lung metastasis, and then carrying out in-vitro cloning, purifying and propagating on the lung-targeting metastatic human hepatoma cell. The invention has wide application potential and potential societal and economic benefits in basic research of hepatoma and clinic previous research of medicines.

Description

technical field [0001] The present invention relates to a lung-targeted metastatic human liver cancer cell line and its establishment method, in particular to a human liver cancer cell line HCCLM3-R-LM1 capable of stably expressing red fluorescent protein (RFP) and capable of lung-targeted metastasis and its establishment method. The invention belongs to the technical field of microbial animal cell lines. Background technique [0002] Primary hepatocellular carcinoma (HCC) is one of the most common malignant tumors in my country and even in the world, and its morbidity and mortality rate ranks second among malignant tumors. There are many methods to treat liver cancer clinically, such as surgical resection, TACE, intratumoral alcohol injection, radiofrequency therapy and radiotherapy, etc., but the long-term efficacy is limited. According to the long-term clinical follow-up data of more than 9,800 liver cancer patients operated by Fudan University Liver Cancer Institute, it...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09C12R1/91
Inventor 吴伟忠陶中华李薇薇孙惠川汤钊猷樊嘉钦伦秀王鲁
Owner ZHONGSHAN HOSPITAL FUDAN UNIV
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