Micromolecular shellfish polypeptide extraction method

An extraction method and small molecule technology, applied in the preparation methods of peptides, chemical instruments and methods, peptides, etc., can solve the problems of undeveloped small molecule shellfish peptides, technology and application gaps, etc., to achieve a broad market space and economical. Value, strong operability, guaranteeing the effect of biological activity

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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the consumption of shellfish is mainly fresh, dried, pickled, and canned, while there are few high-value-added products using shellfish as raw materials. The industry has not yet developed a line for the extraction of small molecule shellfish peptides. The most effective method, the industrial extraction of small molecule shellfish peptides, is still blank in terms of technology and application

Method used

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  • Micromolecular shellfish polypeptide extraction method
  • Micromolecular shellfish polypeptide extraction method

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Effect test

Embodiment 1

[0022] The extraction method of the small molecule shellfish polypeptide of embodiment 1 comprises the following steps:

[0023] (1) Rough washing: use fresh sea red and meretrix clams as raw materials, wash the surface sediment with clean water;

[0024] (2) Shelling: Mix the washed raw materials with shells and tap water heated to 100°C in a weight ratio of 1:1, cook for 12 minutes, and then take out the shellfish;

[0025] (3) Soaking and fine washing: Soak the shellfish in tap water at 2°C for 24 hours, and then wash it repeatedly for 3 times with tap water at 2°C;

[0026] (4) Drying: use a fan to blow air for 10 hours with a 6-level wind force, until the water content of the shellfish is lower than 40%;

[0027] (5) Enzymolysis: Add the dried shellfish and tap water or pure water to the biological enzymatic hydrolysis tank at a weight ratio of 1:2, and add collagenase and papain to the biological enzymatic hydrolysis tank. The addition amount is 0.6% of the weight of t...

Embodiment 2

[0031] The extraction method of the small molecule shellfish polypeptide of embodiment 2 comprises the following steps:

[0032] (1) Rough washing: use fresh razor clams as raw materials, wash the surface sediment with clean water;

[0033] (2) Shelling: Mix the washed raw materials with shells and tap water heated to 100°C in a weight ratio of 1:1, cook for 15 minutes, and then take out the shellfish;

[0034] (3) Soaking and fine washing: Soak the shellfish in tap water at 4°C for 24 hours, then wash it repeatedly 4 times in tap water at 4°C;

[0035] (4) Drying: use a fan to blow air for 8 hours with a wind force of level 6, until the water content of the shellfish is lower than 40%;

[0036] (5) Enzymolysis: Add the dried shellfish and tap water or pure water to the biological enzymatic hydrolysis tank at a weight ratio of 1:1, and add collagenase and papain to the biological enzymatic hydrolysis tank. The addition amount is 0.5% of the weight of the dried shellfish, the...

Embodiment 3

[0040] The extraction method of the small molecule shellfish polypeptide of embodiment 3 comprises the following steps:

[0041] (1) Rough washing: use fresh sea red, meretrix meretrix and razor clam as raw materials, wash the surface sediment with clean water;

[0042] (2) Shelling: Mix the washed raw materials with shells and tap water heated to 100°C in a weight ratio of 1:1, cook for 10 to 20 minutes, and then take out the shellfish;

[0043] (3) Soaking and fine washing: Soak the shellfish in tap water at 1°C for 24 hours, and then wash it repeatedly 5 times in tap water at 1°C;

[0044] (4) Drying: use a fan to blow air for 18 hours with a 6-level wind force, until the water content of the shellfish is lower than 40%;

[0045] (5) Enzymolysis: Add the dried shellfish and tap water or pure water to the biological enzymatic hydrolysis tank at a weight ratio of 1:3, and add collagenase and papain to the biological enzymatic hydrolysis tank. The amount added is 0.9% of the...

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Abstract

The invention discloses a micromolecular shellfish polypeptide extraction method. By scientific and reasonable integration of biological enzymolysis technology and membrane technology, the method includes: subjecting raw materials to biological enzymolysis with a complex enzyme formed by compounding of collagenase and papain; performing separation, concentration and purification sequentially through an ultrafiltration membrane and a nanofiltration membrane specific in molecular weight cutoff to obtain micromolecular shellfish polypeptide containing concentrated liquid; finally subjecting the concentrated liquid to spray drying to obtain a micromolecular shellfish polypeptide product. The extraction method is high in operability; the micromolecular shellfish polypeptide prepared according to the method is high in purity, bioactivity of the micromolecular shellfish polypeptide can be guaranteed effectively, and the micromolecular shellfish polypeptide can be used as a raw material of various high-added-value products subsequently and has a wide market space and high economic value.

Description

technical field [0001] The invention relates to a method for extracting small-molecule polypeptides from seafood, in particular to a method for extracting small-molecule shellfish polypeptides. Background technique [0002] With the rapid development of science and technology and the popularization of nutritional knowledge, as well as the increase of per capita income and consumption level, human beings' requirements for food have transitioned from food and clothing to nutritional level. Natural, healthy, nutritious and safe have become the food industry in the 21st century. major issues of development. Collagen, as an important biological tissue structural protein polypeptide, has attracted more and more people's attention and interest, with huge market potential and high economic value. Compared with macromolecular proteins, collagen small molecule active peptides not only have the characteristics of low synthesis cost, high utilization rate, low toxicity and immunogenici...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34
CPCC12P21/06C07K1/34
Inventor 冯群力张建芳冯志江冯瑜张夏绒
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