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Self-heating amplification detection device and preparation method and use method thereof

A detection device, a heat-type technology, applied in the preparation of self-heating amplification detection devices, self-heating amplification detection devices, and nucleic acid detection systems to achieve the effects of ensuring one-way flow, avoiding biological safety problems, and facilitating control

Pending Publication Date: 2021-05-11
常州市疾病预防控制中心 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the existing technical problems, the present invention intends to solve the technical difficulties of RPA on-site detection of novel coronavirus

Method used

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  • Self-heating amplification detection device and preparation method and use method thereof
  • Self-heating amplification detection device and preparation method and use method thereof
  • Self-heating amplification detection device and preparation method and use method thereof

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Experimental program
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Effect test

Embodiment 1

[0065] The invention provides a self-heating amplification detection device, such as figure 1 , figure 2 As shown, including reaction structure, detection structure, intermediate structure, self-heating structure and sealing structure; among them,

[0066] The intermediate structure includes a glass fiber membrane 16, a water-absorbing layer 17 and a bottom plate 19 arranged from top to bottom;

[0067] The reaction structure includes at least two independent reaction tanks 18, which pass through the glass fiber membrane 16, the water-absorbing layer 17 is fixed on the bottom plate 19, and one side near the detection structure is provided with openings and A sliding partition 25 for opening and closing the opening, and the reaction tank 18 is used for the RPA amplification of the reaction sample;

[0068] The detection structure includes a plurality of independent amplification diffusion areas and independent reporting areas, and the plurality of independent amplification d...

Embodiment 2

[0091] The invention provides a self-heating amplification detection device, such as figure 2 As shown, it includes a closed protective film 15, a glass fiber film 16, a water absorbing layer 17, a bottom plate 19, an iron powder activated carbon heat generating layer 20, and a viscous air-tight layer 21 connected sequentially from top to bottom, and the material of the water absorbing layer 17 is poly One layer of absorbent cotton, the bottom plate 19 is an impermeable layer to protect the upper layer of reaction reagents from contacting the lower layer of iron powder activated carbon heat generation layer 20, and the iron powder activated carbon heat generation layer 20 uses a fiber breathable film as the outer packaging, and a layer is arranged on the inner surface of the breathable film Gelatin to prevent oxygen from entering the interior to cause oxidation reaction when there is no reaction. The specific structure is that the inner layer is wrapped with Fe powder and acti...

Embodiment 3

[0207] The present invention also provides a nucleic acid detection system, such as figure 2 , image 3 As shown, it includes an extraction device and a detection device, the extraction device includes an extraction structure, a storage structure, a magnetic structure and a drive structure, and the detection device is a self-heating amplification detection device as described above;

[0208] The nucleic acid extraction structure comprises an extraction chamber 7, a one-way liquid inlet pipe 3 communicated with the extraction chamber 7, a one-way liquid discharge pipe 4 communicated with the extraction chamber 8, and a one-way liquid inlet pipe 3, a one-way The suction port 1 connected to the end of the discharge pipe 4 away from the extraction chamber 7;

[0209] The storage structure includes a plurality of reagent storage tubes 9 communicating with the extraction chamber 7 and used to store reaction reagents;

[0210] The magnetic structure includes magnetic beads 8 place...

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Abstract

The invention provides a self-heating amplification detection device. The self-heating amplification detection device comprises a reaction structure, a detection structure, an intermediate structure, a self-heating structure and a sealing structure. The self-heating amplification detection device can realize self-heating, can perform amplification reaction detection on different samples, and can also improve the safety performance of the device in the reaction process. The invention further provides a preparation method and a use method of the detection device, and the beneficial effects are as described above. The invention further provides a designed nucleic acid detection system, the nucleic acid detection system comprises an extraction device and a detection device, the RPA technology, closed nucleic acid extraction equipment and a self-heating-dependent colloidal gold test card are combined together, so that an extraction reaction system is completely isolated from the external environment, the biological safety problem can be effectively avoided while the detection time is saved by means of closed normal-temperature amplification, nucleic acid detection can be completely separated from large-scale instruments and equipment, and the on-site detection target of sampling while detecting is realized.

Description

technical field [0001] The invention relates to the field of RNA nucleic acid extraction and on-site detection in the environment, in particular to a self-heating amplification detection device. The invention also relates to a preparation method of a self-heating amplification detection device. The invention also relates to a method for using the self-heating amplification detection device. The invention also relates to a nucleic acid detection system. [0002] technical background [0003] Due to the highly contagious nature of the novel coronavirus, sample collection, transportation, pre-treatment, extraction, and nucleic acid testing are all prone to pollute the environment and lead to staff infection. Therefore, the prevention, control and testing of the novel coronavirus requires three levels of protection. Standard conduct. Relevant specifications and implementation rules have been formulated for sample collection, transportation, extraction and testing. Therefore, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/6804C12Q1/6806C12M1/42C12M1/34C12M1/00
CPCC12Q1/701C12Q1/6804C12Q1/6806C12Q2600/166C12Q2531/119C12Q2537/1376C12Q2522/101C12Q2565/625C12Q2545/113C12Q2563/143C12Q2563/149
Inventor 屠博文王凤鸣陆晓荣徐岩陈聪毛旭建
Owner 常州市疾病预防控制中心
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