40results about How to "Meet the needs of actual testing" patented technology

The invention provides a method for preparing acrylamide complete antigen. The method is as follows: amine uncoupling reaction is directly carried out between N-acryloyloxy succinimide and the surface amino group of carrier protein molecule in a neutral or weakly alkaline phosphate buffer solution, thereby forming acrylamide complete antigen. Polyclonal antibody capable of realizing specificity identification of acrylamide is obtained through a complete antigen immune animal, so as to establish an enzyme immunoassay method suitable for measuring the content of acrylamide in an aqueous solution or food and to provide a corresponding reagent box. The method has simple operation and does not need other reaction reagent, thereby furthest maintaining the structural integrity of acrylamide; moreover, the method ensures that a coupling position is at a maximum distance from a feature group so as to fully expose the feature structure of acrylamide molecule. The established enzyme immunoassay method of acrylamide and the reagent box have strong specificity, high sensitivity and convenient operation, and are suitable to realize massive sample detection which has low cost and is quick and simple.

The invention provides a detection method of hormones in blood, and belongs to the field of the detection of the hormones. The detection method comprises the following steps of mixing and storing the blood and an internal standard solution including a hormone isotope internal standard on a filter paper disc, and preparing an obtained mixture into a dried blood spot. An extracting solution is obtained from the dried blood spot through extraction, and a detection solution is obtained by carrying out derivatization treatment on the extracting solution. The hormone in the detection solution is detected through liquid chromatographic-mass spectrometric detection. In the method provided by the invention, the blood is stored on the filter paper disc, and then the purpose of quickly and effectively carrying out qualitative and quantitative detection on the hormone in the blood is realized through extraction and derivatization processes and by utilizing a liquid chromatographic-mass spectrometric method. As the blood is stored on the filter paper disc and is stored in the form of a solid, a biological safety problem possibly brought about in a transportation process is avoided; moreover, the stability can be greatly improved; therefore, a detected sample is prevented from being polluted by a microorganism.

The invention provides a method for efficiently detecting 15 kinds of bile acid in blood. In the method, serum is directly preserved on a filter paper scrip, and through an efficient extraction process, extracted bile acid is directly injected on a liquid chromatography-mass spectroscopy; 15 kinds of the bile acid in blood can be efficiently detected in a high-throughput and high-repeatability mode, and the method has the wide application value.

The invention relates to a multifunctional food detection system, comprising a power supply module, a system setting unit, a sample pre-treating unit, a detection unit and an information output unit, wherein the power supply module provides electric power for the sample pre-treating unit and a detection host unit, the system setting unit is used for setting parameters of the sample pre-treating unit and the detection unit, the sample pre-treating unit is used for pre-treating a to-be-detected sample, the detection unit is used for detecting and treating the sample, and the information output unit is used for outputting detection results. The multifunctional food detection system provided by the invention is capable of effectively detecting a plurality of components in food, realizes diversification of detection functions and continuity of detection process and meets needs of actual detection.

The invention discloses a flexible micro-column ring array for detecting the contractility of a clot and a preparation method and application of the flexible micro-column ring array. The micro-columnring array is composed of a plurality of micro-column rows arranged periodically, each micro-column row is provided with a plurality of micro-column rings, and each micro-column ring is composed of four or more micro-columns forming a ring shape in a surrounding mode, wherein a circular pore with a certain diameter is formed in the micro-column ring, and when the contractility of the clot is detected, whole blood is dropped into the circular pore of the micro-column ring for blood coagulation detection. According to the invention, a whole blood sample is efficiently fixed in the micro-column ring by utilizing the interfacial tension of a solid-liquid-gas system and the resistance of the micro-column; meanwhile, the micro-column is used as a force sensor to measure the contraction force ofa clot in real time, the detection result is high in sensitivity and low in detection cost, and clinical transformation is easy; besides, platelets do not need to be separated from blood, whole bloodcan be used for detection, the influence of other components in the blood on platelet contraction is fully considered, and the result is more objective and comprehensive.

The invention discloses a liquid-phase detection method for casein phosphopeptides in milk. The liquid-phase detection method comprises the following steps of adding casein phosphopeptides to milk to be detected to prepare a 10-100ppm casein phosphopeptides dispersion solution, carrying out decalcification through cation exchange resin after deproteinization, performing impurity removal and enrichment on an eluant obtained after the deproteinization through the cation exchange resin, carrying out vacuum rotary evaporation on a collecting liquid at the temperature of 60-70 DEG C at the revolving speed of 50-60rpm, and filtering through a membrane to take the collecting liquid as a liquid-phase detection sample; and carrying out liquid-phase detection on the liquid-phase detection sample, and calculating the concentration of the casein phosphopeptides added to the milk by using a peak area method according to an external standard method. The liquid-phase detection method provided by the invention has the advantages that the capital investments of reagents, instruments and the like at the initial stage of the experiment are reduced, the relative simple efficient purification steps are performed, the separation of the casein phosphopeptides is realized, the quantitative accuracy is guaranteed, the interference of impurities such as other proteins and lipids is effectively removed, the final detective sensitivity is improved, and the detection of a sample with low casein phosphopeptides content is beneficial.

The invention discloses an automatic test device and relates to the field of vehicle central electric junction boxes. The automatic test device is arranged between positive and negative electrodes of a power supply and a junction box. The automatic test device comprises a microprocessor, and a current adjustment circuit, a first channel selection circuit, a second channel selection circuit and a current detection circuit, which are respectively connected with the microprocessor, wherein the current adjustment circuit automatically changes a load according to the current test requirement of the microprocessor; the first channel selection circuit is connected with the current adjustment circuit in series and positioned between the positive electrode of the power supply and the junction box; the second channel selection circuit is arranged between the junction box and the negative electrode of the power supply; the current detection circuit is positioned between the microprocessor and the junction box, and used for acquiring an actual current which is currently input into the junction box and transmitting an actual current signal to the microprocessor; and the microprocessor controls motion of the current adjustment circuit according to a comparison result. By adoption of the automatic test device, automatic operation is completely realized. The automatic test device has the characteristics of simplicity of operation, high test efficiency and high test reliability.

The invention discloses an artillery body tube measuring device based on a Beidou radio navigation satellite system (RNSS). The artillery tube measuring device comprises two Beidou antennas installed at the body tube muzzle end and the body tube steering box end respectively with the aid of clamps. The baselines of the two Beidou antennas are in the same direction. The Beidou antenna located at the body tube muzzle end is fixedly connected with a body tube with the aid of the muzzle end clamp. The Beidou antenna located at the steering box end is fixedly connected with the body tube with the aid of the steering box end clamp. A laser alignment device used for measuring the deviation of the baselines of the two Beidou antennas is installed between the steering box end clamp and the muzzle end clamp. The two Beidou antennas are installed at the muzzle end and the steering box end of the body tube respectively through the muzzle end clamp and the steering box end clamp; the muzzle end clamp and the steering box end clamp are used for adjusting the heights and the left-right positions of the two Beidou antennas, and meanwhile, the uniformity of the baselines of the two Beidou antennas is ensured by using the laser alignment device; the influence of human factors is small; the installation accuracy is high; the operation is simple.

The invention belongs to the technical field of detection, and particularly relates to a novel magnetic nano composite material, a magnetic effervescent tablet, a method for detecting BPs and application. The novel magnetic nano composite material is formed by attaching magnetic nano particles to the surface of g-C3N4. The novel magnetic nano composite material provided by the invention can be mixed with an ionic liquid extraction agent to prepare an effervescent tablet, the prepared effervescent tablet can be used for separating and detecting BPs, and compared with the extraction efficiency of singly using the ionic liquid extraction agent, the extraction efficiency is greatly improved. According to the method for detecting BPs provided by the invention, under the assistance of the special magnetic effervescent tablets, the use of a traditional organic dispersing solvent is avoided, and the dispersing and recycling processes of the extracting agent are completed in one step by virtue of an innovative micro-extraction technology, so that the pretreatment time is shortened, and the method is more environment-friendly and efficient.

The invention provides a method for quantitatively detecting mercury ions in a liquid sample and a kit for quantitatively detecting the mercury ions. The absolute quantitative detection of the mercury ions can be performed through a quantitative detection method based on a biosensor and a droplet polymerase chain reaction (PCR) technology and the detection kit, the quantitative detection limit can reach 40 fmol, the sensitivity can reach 10 fmol, and the requirements of actually detecting of the mercury ions can be met. In addition, the method is simple to operate and high in flexibility and is an effective method for performing absolute quantitative detection of the mercury ions.

The invention belongs to the technical field of traditional Chinese medicine quality control, and particularly relates to Qinglongbaihu soup and a quality detection method thereof. Aiming at the problem that a method for detecting the quality of water decoction, decoction pieces, intermediates and material references of the formula Qinglongbaihu soup does not exist at present, the technical schemeof the invention provides the Qinglongbaihu soup with a standardized high performance liquid chromatography fingerprint spectrum, and provides a more accurate quality detection method of the Qinglongbaihu soup. According to the method, the standard fingerprint spectrum of the Qinglongbaihu soup can be obtained, whether the Qinglongbaihu soup test sample is a qualified product or not can be accurately and stably judged, and the content of effective components in the Qinglongbaihu soup can be accurately determined.

The invention provides a double digital PCR fluorescent quantitative detection method for transgenic maize 3272. According to the method, quantitative detection can be directly performed on the transgenic maize 3272 in genomes of samples to be detected, and therefore the standardard curve plotting step in quantitative detection of a conventional PCR method and the sample preprocessing step in existing digital PCR detection are omitted; in addition, transgenic ingredients are calculated according to the proportion of the exogenous gene copy number and the reference gene copy number, and therefore the stability of quantitative detection of the transgenic ingredients can be improved by performing double detection on one reaction system. Through the method, absolute quantitative detection on the transgenic maize 3272 can be achieved, the quantitative detection limitation can reach 0.5%, the sensitivity can reach 0.1%, and the requirements of actual detection of all the transgenic ingredients can be met. In addition, the method is easy to operate and high in flexibility and serves as an effective method for absolute quantitative detection of transgenes.