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Method for determining astragaloside content in Jintian wheat straw formula granules by high performance liquid chromatography

A technology of high-performance liquid chromatography and astragaloside IV, which is applied in the field of component analysis of traditional Chinese medicine, can solve problems such as content differences of active ingredients, limitation of application range, lack of theoretical and scientific research data support for quality control, etc., and achieve simple processing methods, saving time and The effect of high cost and sensitivity

Pending Publication Date: 2020-08-25
JINHUA MUNICIPAL CENT HOSPITAL
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Problems solved by technology

[0005] Due to the large gap with the traditional Chinese medicine water decoction process, there may be a large difference in the content of active ingredients. Jinmaicao formula granules lack a systematic and standardized method for determining the content of active ingredients, resulting in a lack of strong theoretical and scientific data support for its quality control. The scope is limited, therefore, it is urgent to establish a method for determining the active ingredients of golden wheatgrass formula granules

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  • Method for determining astragaloside content in Jintian wheat straw formula granules by high performance liquid chromatography
  • Method for determining astragaloside content in Jintian wheat straw formula granules by high performance liquid chromatography
  • Method for determining astragaloside content in Jintian wheat straw formula granules by high performance liquid chromatography

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Embodiment Construction

[0033] In order to better understand the content of the present invention, further description will be made below in conjunction with specific embodiments and accompanying drawings. It should be understood that these examples are only used to further illustrate the present invention, not to limit the scope of the present invention. In addition, it should be understood that after reading the contents of the present invention, those skilled in the art may make some non-essential changes or adjustments to the present invention, which still belong to the protection scope of the present invention.

[0034] 1. Experimental part

[0035] 1. Instruments and reagents

[0036] High-performance liquid chromatography: Shimadzu LC-20AT high-performance liquid chromatography (Shimadzu Corporation of Japan); Shimadzu PDA diode array detector (Shimadzu Corporation of Japan); Shimadzu chromatography workstation (Shimadzu Corporation of Japan); METTLER AB135-S (Mettler-Toledo Instruments (Sha...

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Abstract

The invention discloses a method for determining astragaloside content in Jintian wheat straw formula granules by high performance liquid chromatography. The method comprises the following steps: (1)preparing a standard solution; (2) preparing a test solution; (3) carrying out liquid chromatography separation; (4) adopting a content calculation method. The sample extraction pretreatment method issimple, and effective components can be fully extracted; the method is accurate, rapid and high in sensitivity; all methodological indexes can meet the requirements of actual detection, and time andcost are saved; the compound to be detected has good linearity (R2 > 0.9990) in a standard curve linear range; intra-day precision of the effective components is less than 1.0%, and the relative standard deviation of the inter-day precision is less than 3.0%; the recovery rate of the effective components of astragaloside is in the range of 90.0%-110.0%.

Description

technical field [0001] The invention relates to the technical field of component analysis of traditional Chinese medicines, in particular to a method for determining the content of astragaloside IV in Jinmaicao formula granules by high performance liquid chromatography. Background technique [0002] Golden Wheat Grass comes from the classic prescription of traditional Chinese medicine "Taiping Huimin Heji Bureau Prescription". The whole prescription is composed of Cadida, Hedyotis, Honeysuckle, Platycodon grandiflorum, Astragalus, Lily, and Atractylodes macrocephala. It has the effects of clearing the lungs and reducing phlegm, relieving cough and asthma, and is a traditional Chinese medicine prescription commonly used in the clinical treatment of chronic obstructive pulmonary disease. . [0003] Traditional Chinese medicine formula granules are a new type of granular preparation developed in recent years by using modern production technology, using Chinese herbal medicine ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/30G01N30/32G01N30/34G01N30/74
CPCG01N30/02G01N30/06G01N30/30G01N30/32G01N30/34G01N30/74G01N2030/324
Inventor 颜梅梅新路
Owner JINHUA MUNICIPAL CENT HOSPITAL
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