Detection method of hormone in blood

Abstract

The invention provides a detection method of hormones in blood, and belongs to the field of the detection of the hormones. The detection method comprises the following steps of mixing and storing the blood and an internal standard solution including a hormone isotope internal standard on a filter paper disc, and preparing an obtained mixture into a dried blood spot. An extracting solution is obtained from the dried blood spot through extraction, and a detection solution is obtained by carrying out derivatization treatment on the extracting solution. The hormone in the detection solution is detected through liquid chromatographic-mass spectrometric detection. In the method provided by the invention, the blood is stored on the filter paper disc, and then the purpose of quickly and effectively carrying out qualitative and quantitative detection on the hormone in the blood is realized through extraction and derivatization processes and by utilizing a liquid chromatographic-mass spectrometric method. As the blood is stored on the filter paper disc and is stored in the form of a solid, a biological safety problem possibly brought about in a transportation process is avoided; moreover, the stability can be greatly improved; therefore, a detected sample is prevented from being polluted by a microorganism.

Description

technical field [0001] The invention relates to the field of hormone detection, in particular to a method for detecting hormones in blood. Background technique [0002] Hormones play an important role in regulating various physiological processes of the body, such as metabolism, growth, development, and reproduction, and are important substances in life. The content of hormones in the human body is very low, but the regulation is extremely significant, and changes in the body have a great impact on health. At present, clinical hormone testing has also been widely carried out. However, when it comes to the detection of hormones in blood, due to the inconvenience of long-distance transportation and storage of blood, and the impact of biosafety problems in the process of transportation and storage, the existing blood hormone detection methods are greatly limited. , and the existing traditional methods are not stable, low sensitivity, poor specificity, easy to produce cross-co...

AI Technical Summary

Problems solved by technology

Aiming at the biological safety problems that may be brought about in the process of blood transportation, and the shortcomings of being susceptible to microbial contamination, difficult to store and long-distance transportation, the blood is made into dried blood sheets to improve its safety and stability and avoid microbial contamination. It effectively solves the problem that blood cannot be transported and stored over long distances in hormone detection, and the detection method also has the advantages of high sensitivity and good stability

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