Method for simultaneously measuring seven index components in formula granules of Huagai powder for treatment of wind-cold-caused common cold and asthma by using high performance liquid chromatography

Abstract

The invention discloses a method for simultaneously measuring seven index components in formula granules of Huagai powder for treatment of wind-cold-caused common cold and asthma by using high performance liquid chromatography. The seven index components include ephedrine hydrochloride, pseudoephedrine, amygdalin, glycyrrhizic acid, liquiritin, sanggenon C, and sanggenon D. The method comprises the following steps: step one, preparing a standard solution; step two, preparing a test sample solution; step three, carrying out liquid chromatogram separation; step four, performing a content calculation method. According to the invention, the pretreatment method of sample extraction is simple and the index components can be extracted fully. The method is performed accurately and rapidly with high sensitivity and low cost; and various methodological indexes can satisfy the actual detection demands. Seven measured compounds have high linearity in a standard curve linear range, wherein R2 is larger than 0.9990; the within-day precision relative standard offsets of all components are less than 1.0%; and the day-to-day precision relative standard offsets are less than 3.0%. The recovery ratesof the seven index components are in a range of 90.0% to 110.9%.

Description

technical field [0001] The invention relates to the technical field of component analysis of traditional Chinese medicines, in particular to a method for simultaneously determining seven index components in Huagai powder formula granules by using high performance liquid chromatography. Background technique [0002] Hua Gai San comes from the classic prescription of traditional Chinese medicine "Taiping Huimin Heji Bureau Prescription". The whole prescription is composed of ephedra, perilla, almond, tangerine red, Morus alba, Poria cocos, and licorice. It has the effects of clearing the lung and reducing phlegm, relieving cough and asthma, and is a traditional Chinese medicine prescription with definite curative effect on chronic obstructive pulmonary disease. [0003] Traditional Chinese medicine formula granules are a new type of granular preparation developed in recent years by using modern production technology, using Chinese herbal medicine pieces as raw materials, addin...

AI Technical Summary

Problems solved by technology

[0005] Due to the large gap with the traditional Chinese medicine water decoction process, there may be a large difference in the content of active ingredients. The existing technical methods can only measure 2 to 3 index components in the Huagai powder formula granules at the same time, and the Huagai powder formula granules lack systematic and standardized effective The determination method of component content leads to the lack of strong theory and scientific research data support for its quality control, and the scope of application is limited to a certain extent. Therefore, it is urgent to establish a method for simultaneously determining multiple index components of Huagai powder formula granules

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