58results about How to "Column depression" patented technology

The invention discloses a method for preparing surface mesoporous silica core-shell microspheres. The method comprises: selecting monodisperse non-porous silica microspheres with the particle size of 1 to 3 microns, dispersing quaternary ammonium salt B and quaternary ammonium salt A dispersing agents in ethanol water, dispersing the silica microspheres in water, adding a mixed surface active agent solution, regulating a pH value to be 7.5 to 10 by using ammonia water, adding tetraethoxysilane and/or tetramethoxysilane solution, washing, drying, calcining and removing a structure-directing agent, thereby obtaining the surface mesoporous silica core-shell microspheres. By utilizing the quaternary ammonium salt with two different carbon chain lengths as co-structure-directing agent, the core-shell microspheres with a relatively larger radioactive mesoporous structure are prepared; by regulating the proportion of two quaternary ammonium salt structure-directing agents, the mesoporous aperture is controllable in a range of 4 to 20 nm, the obtained radioactive mesoporous structure increases effective specific surface areas of the microspheres, and the application of the microspheres in adsorption, catalysis and separation analysis is improved.

The invention provides molecularly imprinted polymeric microspheres for phenol. The polymeric monomer of the polymeric microspheres is methacrylic acid or 4-vinylpyridine and the specific surface area of the polymeric microspheres is 23.08-62.33m<2>/g. The invention also provides a method for preparing the polymeric microspheres and the use of the polymeric microspheres in collection or detection of phenol compounds. In addition, the invention also provides a chromatographic column and a solid phase extraction cartridge filler. The filler is the molecularly imprinted polymeric microspheres. The molecularly imprinted polymeric microspheres are simple and convenient in preparation, have an adsorption selectivity of 8.25 to 44.55 mg/g, reach a balance within 6 hours and have the advantages of high absorption selectivity and high absorption speed.

A comprehensive utilization process of a glutamic acid crystal mother liquid comprises the following steps: one, leading the glutamic acid crystal mother liquid into a membrane separation system, allowing solid particles, thalli, proteins, polysaccharides and a colloid in the mother liquid to be intercepted by a membrane, and making glutamic acid left in a membrane filtrate; and allowing the membrane filtrate to go to a next step for treatment; two, leading the membrane filtrate into an ion exchange system, adsorbing glutamic acid by an ion exchange resin, when the content of glutamic acid in an ion exchange tail liquid is not more than 0.2%, stopping column-loading, adding an eluting agent, eluting, allowing glutamic acid to go into the eluate, collecting the eluate, and recycling glutamic acid from the eluate. The membrane separation technology and the ion exchange technology are combined, recycling of glutamic acid from the crystal mother liquid is realized, at the same time, a protein feed is prepared from the thalli and the proteins intercepted by the membrane, an organic fertilizer is prepared from the ion exchange tail liquid through slurry-spraying granulation, the whole process allows useful substances in the crystal mother liquid to be totally recycled, comprehensive utilization of the resources is thoroughly achieved, waste is turned into treasure, and significant economic benefits are achieved.

The invention discloses a novel method for preparing macroporous silicon dioxide@porous polymer core-shell microspheres. Non-porous silica microspheres are used as a core, octadecyltrichlorosilane isused for performing the surface hydrophobic modification, PVA and SDS are used as a dispersing agent, monomers GMA, a cross-linking agent EDMA and a pore forming agent are added to prepare the silicondioxide@porous polymer core-shell microspheres with a macroporous structure through an emulsion polymerization process. The preparation method is simple in preparation process, mild in conditions, and easy in mass production; and the surface of the prepared core-shell microspheres is a porous organic polymer which has higher acid and alkali resistance, the application range of core-shell fillersis enlarged, a larger aperture structure can be obtained, and the prepared core-shell microspheres are particularly suitable for the isolated analysis of biological molecules.

The invention discloses a detection method of salbutamol sulfate-related substances. The detection method qualitatively or quantitatively detects the salbutamol sulfate-related substances with high performance liquid chromatography; detection conditions comprise a chromatographic column: octyl-bonded silica gel column, a detection wavelength: 210-400 nm, and mobile phases comprising a mobile phaseA and a mobile phase B, wherein the mobile phase A is an aqueous phase containing a buffer solution and having pH of 1.5-4.0, further having pH of 2.0-3.0; the mobile phase B mainly comprises methanol or acetonitrile; the mobile phases are eluted by gradient elution; and detection steps are as follows: (1) preparing a test substance solution and a reference substance solution; and (2) respectively sampling and detecting the test solution and the reference solution. The detection method of the salbutamol sulfate-related substances adopted by the invention can ensure effective separation of therelated substances.

The invention discloses an integral separating dielectric of polymer and making method, which has regular three-dimensional frame structure constituted by polymer frame and through-out hole, wherein the nanometer grade middle-hole is formed on the surface of the polymer frame and/or in the polymer frame; the through-out hole is between 0. 1um and 10um and the middle hole is between 0nm and 50 nm; the frame size is 0. 2-2um. The invention has regular and sequent dielectric structure to be separated after doing chemical decoration for frame surface, which has huge applying potential in the macromolecular separating and purifying and solid phase extracting domain.

The invention discloses a polymer microsphere and preparation method and application thereof. Polystyrene is used for making a base sphere with the surface comprising a condensed-nuclei aromatic structure in pi-pi interaction with fullerene, and the structure is obtained by substituting a hydrogen atom on a benzene ring contained in the base sphere by chloromethyl and reacting with condensed-nuclei aromatics. The method includes: using a monodispersed polystyrene microsphere as the base sphere, mixing with catalyst SnCl4, adding aliphatic halogenate varsol, slowly dripping chloromethylation reagent while stirring for substitution reaction, allowing the reacted microsphere to react with the condensed-nuclei aromatics, and washing and drying the obtained microsphere to obtain a polymer microsphere with a fullerene separated liquid chromatographic stationary phase serving as a mobile phase. The preparation method of the surface modified polystyrene polymer microsphere only needs two steps, is simple in process, easy to repeat, suitable for massive production of fullerene and has the advantages of high separation efficiency and effectiveness.

The invention relates to high-performance liquid chromatography stationary phases, in particular to a hydrophilic interaction chromatography/ ion exchange chromatography mixed stationary phase and preparation and application of the hydrophilic interaction chromatography/ ion exchange chromatography mixed stationary phase. The structure of the hydrophilic interaction chromatography/ ion exchange chromatography mixed stationary phase is shown as the formula 1, and please see the formula 1 in the specifications, wherein X is -OCH3 or -OCH2CH3, Y1 is F or Cl or Br or I or BF4 or Tf2N or CF3SO3 or SCN or NO2 or NO3, Y2 is -NHCH3 or -N (CH3)2 or -N+ (CH3)3Y1 or -NHCH2CH3 or -N (CH2CH3)2 or -N+ (CH2CH3)3Y1, and n=2 or 3. According to the prepared mixed type stationary phase, raw materials are easy to obtain and the preparation procedure are simple; and the prepared mixed type stationary phase has good retaining performance and separation selectivity for multiple saccharides and has good application and popularization prospects.

The invention provides a 2, 4, 6-trichlorophenol molecular engram microsphere polymer. The invention also provides a method for preparing the 2, 4, 6-trichlorophenol molecular engram microsphere polymer. The invention also provides application of the 2, 4, 6-trichlorophenol molecular engram microsphere polymer in enriching and/or separating 2, 4, 6-trichlorophenol. The invention also provides the application of the 2, 4, 6-trichlorophenol molecular engram microsphere polymer in monitoring and/or processing the 2, 4, 6-trichlorophenol in an environmental water sample. The invention also provides a method for detecting and/or processing the 2, 4, 6-trichlorophenol in a sample through the 2, 4, 6-trichlorophenol molecular engram microsphere polymer. The polymer has simple and convenient preparation, an even grain size, a large specific surface area and pore volume, and high absorption selectivity. The polymer can be used for carrying out the experiment directly in the water environment without excessive other organic agents. The polymer is environmentally friendly and has a high absorption speed.

The invention relates to the technical field of separation and purification of natural functional ingredients, and particularly discloses a method for purifying a capsaicin monomer by utilizing a reverse-phase resin. The method comprises the following steps: (1) carrying out preliminary purification on a crude capsaicin product obtained by virtue of an aqueous two-phase method by utilizing a macroporous resin; (2) further carrying out purification on the capsaicin by utilizing the novel reverse-phase resin; (3) crystallizing. The method has the beneficial effects that compared with reverse-phase carbon-18 silica gel fillers, the novel reverse-phase resin disclosed by the invention is low in price, low in column pressure and large in adsorption capacity; the whole process is simple and easy to operate and less in investment; meanwhile, a toxic organic solvent is not adopted, so that the production cost is greatly lowered and the purification efficiency is improved. The method is suitable for industrial production.

The invention relates to the technical field of drug analyzing, in particular to a method for detecting effective components in asthma pellets. A quantitative and qualitative detecting method for theeffective components in the asthma pellets is provided and used for qualifying ten effective components in the asthma pellets and quantifying seven effective components; the separation degree of the components is not lower than 1.5. With the adoption of the method, the seven effective components can be quantified, and the standard recovery rate ranges from 97.29 to 103.78%, so that the method is high in accuracy.

The invention relates to a padding used to do molecule brand solid-phase extraction and it's preparing method. The carrier of the padding is glass bead which has been surface modified. The preparing method of padding is using liquid film method to form a polymeric film layer with molecule brand at the surface of carrier.

The invention discloses a preparation method and application of an amitraz molecular imprinting monolithic column. The preparation method comprises the following steps: by taking amitraz as a template molecule, dissolving the template molecule into a mixed solution of acetonitrile and dodecyl alcohol, adding a functional monomer, pre-polymerizing at a low temperature, further adding a cross-linking agent and an initiator, introducing nitrogen, filling into a chromatographic column hollow tube of which the lower end is already sealed, sealing the upper end of the chromatographic column hollow tube, and initializing polymerization, thereby obtaining the molecular imprinting monolithic column. After the functional monomer, a pore-foaming agent and the template molecule which are not polymerized are washed off from the column by using a mixed solution of methanol and acetic acid, the molecular imprinting monolithic column which has a good adsorption effect and an enrichment effect on amitraz is obtained. Compared with a conventional body polymerization method, the preparation method has the characteristics that the preparation method is simple and convenient, the aftertreatment is simple, the recognition property is good, the cost is low, required instruments and reaction conditions are easy to achieve, and the like. The molecular imprinting monolithic column prepared by using the preparation method is capable of separating and detecting residual amitraz in foods.

The invention provides an establishment method of a scorzonera fingerprint spectrum. The establishment method comprises the following specific steps: preparing a test solution by scorzonera, and carrying out fractionation detection under a certain condition by a high performance liquid chromatograph to obtain the scorzonera fingerprint spectrum. The invention further provides a scorzonera standard fingerprint spectrum obtained by the method. The standard fingerprint spectrum can provide reliable basis for scorzonera identification and internal mass control. The establishment method is simple and convenient to operate, high in stability, good in reproducibility and multiple in characteristic peaks. By utilizing the establishment method, the quality of scorzonera medicinal materials can be comprehensively and accurately evaluated.

The invention provides a method for detecting the content of EDTA-2Na (Ethylene Diamine Tetraacetic Acid-2Na) in sodium hyaluronate. The method comprises the following steps: firstly, degrading a sodium hyaluronate sample by adopting hyaluronidase, and then determining the content of the EDTA-2Na by adopting a conventional ferric trichloride derivation method through high performance liquid chromatography. According to the method, specific hyaluronidase is screened, so that the viscosity of a high-concentration sample can be effectively reduced, the column pressure is reduced, and test equipment is protected; and meanwhile, the produced product does not influence the detection of a detection target, and the detection sensitivity can be improved. A more accurate method is provided for production and detection of sodium hyaluronate, and the method has important significance in improving the quality of sodium hyaluronate.

The invention discloses a liquid chromatography-tandem mass spectrometry detection method for emodin and aloe-emodin in mouse urine, which comprises the following steps of S1, unfreezing the collectedurine, and recovering to room temperature, S2, taking the urine obtained in the step 1, and adding deionized water for mixing, S3, adding an internal standard into the mixed solution, S4, filtering the mixed solution, and S5, taking the filtered mixed solution for determination by a liquid chromatography-mass spectrometer, the liquid chromatography mobile phase being a water-acetonitrile system,the liquid chromatography column being a C18 reversed-phase liquid chromatography column, and the flow velocity of the liquid chromatography column being not greater than 0.5 mL/min. The method has the advantages of high sensitivity, simple operation, high stability and reliable result.

The invention discloses silicon-based anion exchange resin and a preparation method thereof, and relates to the technical field of anion exchange resin. The resin is obtained by chloromethylation andre-amination reaction of silicon-based white balls, and has a trimethylamine type quaternary ammonium salt functional group; the total exchange capacity of the resin is 0.8-1.0 meq/g; the particle size of the resin is 75-150 [mu]m. The porosity is 47 to 48 percent; the water absorption swelling rate is 0%; the particle size of the obtained resin is consistent with that of silicon dioxide and ranges from 75 micrometers to 150 micrometers. According to the invention, the resin is rich in pore structure, so that the problem that traditional resin is too slow in dynamics is solved, and meanwhilethe problems of too large column pressure, poor resin mechanical performance and the like caused by too high water absorption swelling rate of organic resin are solved.