Method for preparing octyl group micro column liquid phase chromatograph integrate column

A liquid chromatography and monolithic column technology, which is applied in the field of preparation of microcolumn liquid chromatography monolithic columns, can solve problems such as low chromatographic capacity, and achieve the effects of simple preparation, low column pressure and strong stability
CN1236308CInactive Publication Date: 2006-01-11XIAMEN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
XIAMEN UNIV
Publication Date
2006-01-11
Estimated Expiration
Not applicable · inactive patent

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Abstract

The process of preparing integral octyl group micro liquid phase chromatographic column includes the following steps: pre-bonding one layer of silane matter with olefin radical to the inner wall of capillary; preparing integral octyl methacrylate-glycol dimethyl methacrylate column; injecting the mixture of reactant monomer and pore creating agent into bonded capillary; reaction in water bath and flushing with methanol as flowing phase; and performing mu-HPLC separating operation. The present invention facilitates the reaction between gamma-MAPS and silicohydroxyl in the wall of the capillary and obtains integral column with excellent permeability, high space utilization, low column pressure, powerful stability, simple preparation and easy altering in performance and length.
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Description

technical field

[0001] The invention relates to a preparation method of a microcolumn liquid chromatography monolithic column, in particular to a preparation method of an octyl microcolumn liquid chromatography monolithic column. Background technique

[0002] Micro-column high-performance liquid chromatography (μ-HPLC), also known as capillary liquid chromatography (c-HPLC), is a chromatographic micro-separation technology developed in recent years. It has the advantages of low pollution, high separation efficiency, and easy online combination with other detection methods, so it will play an important role in the fields of biochemistry, medicine, and environment. However, the preparation of μ-HPLC chromatographic columns often adopts the packing method, and there is a large column pressure under high flow conditions, which has a great impact on the service life of the chromatographic column and the maintenance of the instrument. If the flow rate is too small, the analysis t...

Claims

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