Method for detecting effective components in asthma pellets

A detection method and technology of active ingredients, which is applied in the field of detection of active ingredients in asthma granules, can solve the problems of excipient interference, complex components, and inability to effectively quantify or qualitatively detect the active ingredients of asthma granules, and achieve good accuracy.

Active Publication Date: 2018-03-06
JIANGSU KANION PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the complex composition of asthma granules, the active ingredients mainly include glycosides, alkaloids, lignans, volatile oils, etc., and a large amount of excipients β-cyclodextrin
Due to the complex detection target components, the physical and chemical properties of each active ingredient are quite different, and there are a large number of excipients
With the current detection method, it is still impossible to carry out effective quantitative or qualitative detection of the active ingredients in asthma granules

Method used

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  • Method for detecting effective components in asthma pellets
  • Method for detecting effective components in asthma pellets
  • Method for detecting effective components in asthma pellets

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Embodiment 1 qualitative detection

[0058] 1 Chromatographic conditions

[0059] The chromatographic column is Kromasil 100-3.5C18 (4.6X150mm, 3.5μm), and the mobile phase is methanol (A)-acetonitrile-(B)-water (C). The elution program is: 0~2min, 15%A; 2~16min , 15%~54%A, 16~30min, 54%~72%A; 30~36min, 72%~98%A; 0~36min, 2%B; column temperature: 40℃; flow rate 1.0mL·min -1 ; Detection wavelength: 210nm (laetrilein, magnolanin), 240nm (morroniside, loganin, acteoside, 5-O-visamigoside, schisandrin A), injection volume 5 μL .

[0060] 2 Selection of reference objects

[0061] Select 5-O-visamigoside with moderate retention time and better separation as a reference.

[0062] 3 Preparation of the test solution

[0063] Take the asthma granules (batch number: 141101) under the item of difference in loading amount, mix well, take an appropriate amount, grind finely, take about 1.0 g, weigh it accurately, put it in a stoppered Erlenmeyer flask, add 25 mL of 50% methanol ...

Embodiment 2

[0078] Embodiment 2 quantitative detection

[0079] 1 Chromatographic conditions

[0080] The chromatographic column is Kromasil 100-3.5C18 (4.6×150mm, 3.5μm), and the mobile phase is methanol (A)-acetonitrile-(B)-water (C). The elution program is: 0~2min, 15%A; 2~ 16min, 15%~54%A, 16~28min, 54%~72%A; 0~28min, 2%B; column temperature: 40℃; flow rate 1.0mL·min-1; detection wavelength: 210nm (amygdalin , magnolanin), 240nm (morroniside, loganin, acteoside, 5-O-visamigoside, schisandrin A), injection volume 5 μL.

[0081] 2. Preparation of reference substance stock solution

[0082] Precisely weigh an appropriate amount of morroniside, amygdalin, loganin, acteoside, 5-O-visamigoside, magnolanin and schisandrin A reference substance, and add 50% methanol to make a mixed standard Stock solution (morroniside content, amygdalin, loganin content 640.93 μg·mL -1 , Actinoside content 171.78μg·mL -1 , 5-O-Visamidol glycoside content 283.62μg·mL -1 , magnolanin, schisandrol A conten...

Embodiment 3

[0114] Embodiment 3 missing square detection

[0115] Five control medicines lacking bitter almond, Magnolia magnolia, Cornus officinalis, Fangfeng and Schisandra chinensis were prepared respectively. The reference substance solution prepared in Example 2 and the test sample (asthma granules, lot number 141101.) were used as contrasts to perform chromatographic detection and observe the difference in chromatograms.

[0116] 1 Chromatographic conditions

[0117] The chromatographic column is Kromasil 100-3.5C18 (4.6×150mm, 3.5μm), and the mobile phase is methanol (A)-acetonitrile-(B)-water (C). The elution program is: 0~2min, 15%A; 2~ 16min, 15%~54%A, 16~28min, 54%~72%A; 0~28min, 2%B; column temperature: 40℃; flow rate 1.0mL·min-1; detection wavelength: 210nm (amygdalin , magnolanin), 240nm (morroniside, loganin, acteoside, 5-O-visamigoside, schisandrin A), injection volume 5 μL. Test results such as Figure 4~5 .

[0118]It can be seen that according to the method provide...

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Abstract

The invention relates to the technical field of drug analyzing, in particular to a method for detecting effective components in asthma pellets. A quantitative and qualitative detecting method for theeffective components in the asthma pellets is provided and used for qualifying ten effective components in the asthma pellets and quantifying seven effective components; the separation degree of the components is not lower than 1.5. With the adoption of the method, the seven effective components can be quantified, and the standard recovery rate ranges from 97.29 to 103.78%, so that the method is high in accuracy.

Description

technical field [0001] The invention relates to the technical field of drug analysis, in particular to a method for detecting active ingredients in asthma granules. Background technique [0002] Asthma granule is a traditional Chinese medicine for the treatment of allergic asthma developed by Jiangsu Kangyuan Pharmaceutical Co., Ltd. The drug filed a patent application on December 6, 2015, and the application number is CN201510885057.9. The medicine is made from Fangfeng, perilla leaves, ephedra, schisandra, ginkgo, bitter almond, aster, cornus, magnolia. The specific preparation method is to extract volatile components from perilla leaves and Magnolia magnolia by steam distillation, and use β-cyclodextrin to enclose them for later use. Combine the medicinal residues with Fangfeng, Sun-baked ephedra, and bitter almonds, and decoct them with water for 2-3 times respectively. , each time for 1-3 hours, combine the extracts, concentrate them into a clear paste with a relative ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/06
CPCG01N30/06G01N30/88
Inventor 萧伟林夏胡军华于桂芳潘有智李家春黄文哲王振中
Owner JIANGSU KANION PHARMA CO LTD
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