Method for purifying capsaicin monomer by utilizing reverse-phase resin

Abstract

The invention relates to the technical field of separation and purification of natural functional ingredients, and particularly discloses a method for purifying a capsaicin monomer by utilizing a reverse-phase resin. The method comprises the following steps: (1) carrying out preliminary purification on a crude capsaicin product obtained by virtue of an aqueous two-phase method by utilizing a macroporous resin; (2) further carrying out purification on the capsaicin by utilizing the novel reverse-phase resin; (3) crystallizing. The method has the beneficial effects that compared with reverse-phase carbon-18 silica gel fillers, the novel reverse-phase resin disclosed by the invention is low in price, low in column pressure and large in adsorption capacity; the whole process is simple and easy to operate and less in investment; meanwhile, a toxic organic solvent is not adopted, so that the production cost is greatly lowered and the purification efficiency is improved. The method is suitable for industrial production.

Description

(1) Technical field [0001] The invention relates to the technical field of separation and purification of natural functional components, in particular to a method for purifying capsaicin monomer with a reversed-phase resin. (2) Background technology [0002] The spicy ingredients in capsicum fruit are a class of capsaicinoids mainly capsaicin, which are: capsaicin, dihydrocapsaicin, nordihydrocapsaicin, homocapsaicin and p-methyl capsaicin, p-methyl capsaicin, p- Methyl capsaicin chain, p-methyl capsaicin saturated chain hydrocarbon, p-tolyl capsaicin, etc., among which capsaicin is the main one, and capsaicin and dihydrocapsaicin account for about 90% of the total above. Capsaicin has pharmacological effects such as analgesia, anti-inflammatory, appetite-stimulating, digestion-improving, antibacterial and insecticidal, and selectivity to neurotransmitters. However, some phenolic substances in crude capsaicin can antagonize the effect of capsaicin on nerve skin, so this cr...

AI Technical Summary

Problems solved by technology

However, some phenolic substances in crude capsaicin can antagonize the effect of capsaicin on nerve skin, so this crude product cannot induce neuropeptide activity clinically, but aggravates pain and inflammation, so capsaicin must be purified before it can be used.

Since capsaicin and its analogs are weakly polar compounds, the difference in their chemical structures is only in the carbon chain structure, so they have very similar physical and chemical properties, so that it is difficult to completely identify capsaicin and its analogs. separate

[0003] The existing purification and preparation methods of capsaicin monomer mainly include silica gel column chromatography, counter-current chromatography, and simulated moving bed method. Zhejiang University uses simulated moving bed chromatography to directly load the capsaicin compounds that have been initially extracted and purified. To the simulated moving bed, high-purity capsaicin monomer and dihydrocapsaicin monomer [Journal of Chromatography A, 1187 (2008) 281-284] have been obtained, although this method can be operated continuously, the processing capacity is very small, and the period Long, expensive equipment

Peng Aihua et al. used high-speed countercurrent chromatography to separate capsaicin and dihydrocapsaicin with a purity greater than 90% from a mixture of capsaicin and dihydrocapsaicin with a purity of 98.5 and 97.8%, respectively [Journal of Separation Science 2009, 32, 2967 -2973], but this method is not only expensive for separation equipment but also requires higher purity of capsaicin and dihydrocapsaicin in raw materials

Chinese patent document 200610068854.9 discloses a method for purifying capsaicin by using affinity chromatography. The crude product of capsaicin compounds is loaded onto a pre-treated affinity chromatographic column. The affinity chromatographic column is silica gel treated with silver nitrate prepared, and then eluted in two steps with a mixed solvent of low-carbon chain halogenated hydrocarbons and low-carbon chain esters to obtain capsaicin crystals with a purity of more than 98%. This method requires expensive silver Salt treatment, silica gel cannot be regenerated and reused, and a large amount of flammable and explosive organic solvents are used in the extraction process, causing pollution to the environment

However, the latter two methods have small processing capacity, long cycle time and expensive production equipment, which are not conducive to large-scale industrial production.