Method for preparing organic arsines molecular imprinting monolithic column and application thereof

Abstract

Disclosed is a method for preparing an organic arsines molecular imprinting monolithic column and application thereof. The method comprises the steps of taking organic arsines veterinary drug carbarsone / nitarsone as template molecules, dissolving the template molecules in a mixed solution of carbinol and dodecanol, adding functional monomers, adding a cross-linking agent and a initiating agent after the mixture is prepolymerized at a low temperature, injecting the mixture into a lower-end-sealed empty column tube of a solid phase extraction column after nitrogen is led, sealing the upper end of the empty column tube of a solid phase extraction column, triggering polymerization at a temperature ranging between 10 DEG C and 30 DEG C for 12 hours to 60 hours, and then obtaining the molecular imprinting monolithic column. The molecular imprinting monolithic column which has good adsorption and enrichment effects on the organic arsines veterinary drug is obtained after the column is eluted from a porogen and the template molecules by a mixed solution of the carbinol and acetic acid. Compared with a traditional mass polymerization method, the method has advantages that the preparation and the postprocessing are simple, recognition is good, cost is low, and the required apparatus and reaction conditions can be acquired easily. The molecular imprinting monolithic column provided by the method can separate, enrich and purify the organic arsines veterinary drug remained in animal origin foods.

Description

technical field

[0001] The invention relates to the field of analytical chemistry, in particular to the preparation of an organic arsine molecularly imprinted monolithic column and its application in the analysis of organic arsine veterinary drug residues in animal foods. Background technique

[0002] Organic arsines are a class of widely used antibacterial and growth-promoting feed additive drugs. Due to their good effects in improving production performance, they have become one of the most widely used and demanded feed additive drugs in the world. In the 1940s, Fleming Pharmaceutical Company of the United States first successfully developed the organic arsine drug p-aminophenylarsine. In 1993, my country approved the use of this drug for pigs and chickens, and in 1996, it approved the use of roxarsine. . In addition, this type of drug also has a good control effect on various parasites such as histomonas, amoeba, trichomonas, balancidia, eperythrozoon, coccidiosis, piripl...