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Method for preparing organic arsines molecular imprinting monolithic column and application thereof

A technology of molecular imprinting and organic arsine, which is applied in the field of analytical chemistry, can solve the problems of poor tolerance to environmental conditions, complex antibody preparation process, and long preparation cycle, and achieve strong tolerance, easy promotion and popularization, and simple preparation process easy effect

Inactive Publication Date: 2012-07-11
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, although the SPE column based on biological antibodies can specifically identify target molecules, the preparation process of antibodies is complicated, high in cost, long in preparation cycle and poor in tolerance to environmental conditions

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (one). Preparation of Molecularly Imprinted Monolithic Columns of Organic Arsines

[0030] Weigh 0.5mmol of the template molecule (carbazine) and dissolve it in 8mL of a mixed solution of methanol and dodecanol (1:2, V / V), add 2mmol of the functional monomer (methacrylic acid), and place it in a refrigerator at 4°C for 24h. Make the monomer and the template molecule fully interact to complete the pre-polymerization process. Then add cross-linking agent trimethylolpropane trimethacrylate 10mmol and initiator benzoyl peroxide and N, N-dimethylaniline each 0.4mmol, after ultrasonic 5min, pass into nitrogen 5min, under nitrogen atmosphere Pour the mixed solution into a 3mL SPE empty column tube covered with a bottom sieve plate and the bottom end is closed, and then seal the other end of the column. Place the formed mixed solution at 15°C to initiate polymerization for 24 hours. After the polymerization is completed, open the seals at both ends of the column, cover the up...

Embodiment 2

[0045] (one). Preparation and Characterization of Organic Arsine Molecularly Imprinted Monolithic Columns

[0046] The basic process is the same as in Example 1, the porogen is a mixed solution of methanol and dodecanol (1:3, V / V) 5mL, the prepolymerization time is 48h, the functional monomer acrylamide is 3mmol, and the crosslinking agent trimethylol Propane trimethacrylate 15mmol, initiator benzoyl peroxide and N, N-dimethylaniline 0.6mmol each, polymerization temperature is 20 ℃, time is 18h, the column capacity of obtained monolithic column to organic arsine class drug and imprinting factors are shown in Table 3.

[0047] Table 3 Column capacities and imprinting factors of molecularly imprinted monolithic columns

[0048] drug type Column capacity ( mu g / g) imprinting factor Carbassine 965.9 3.1 Roxarsone 798.5 1.7 Nitrasinic acid 835.7 2.6 p-aminophenylarsine 638.4 2.4

[0049] (two). Determination of recovery rate of m...

Embodiment 3

[0054] (one). Preparation and Characterization of Organic Arsine Molecularly Imprinted Monolithic Columns

[0055] The basic process is the same as in Example 1, the porogen is 10mL methanol and dodecanol mixed solution (1:5, V / V), the prepolymerization time is 36h, the functional monomer is 4mmol of 4-vinylpyridine, and the crosslinking agent 20 mmol of trimethylolpropane trimethacrylate, 0.8 mmol of benzoyl peroxide and 0.8 mmol of N, N-dimethylaniline as initiators, 25° C. for 12 hours for polymerization, and the obtained monolithic column is suitable for organic arsines The column capacity and imprinting factor of the drug are shown in Table 5;

[0056] Table 5 Column capacities and imprinting factors of molecularly imprinted monolithic columns

[0057] drug type Column capacity ( mu g / g) imprinting factor Carbassine 953.4 2.9 Roxarsone 786.3 1.8 Nitrasinic acid 811.5 2.7 p-aminophenylarsine 674.2 2.2

[0058] (two). Det...

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Abstract

Disclosed is a method for preparing an organic arsines molecular imprinting monolithic column and application thereof. The method comprises the steps of taking organic arsines veterinary drug carbarsone / nitarsone as template molecules, dissolving the template molecules in a mixed solution of carbinol and dodecanol, adding functional monomers, adding a cross-linking agent and a initiating agent after the mixture is prepolymerized at a low temperature, injecting the mixture into a lower-end-sealed empty column tube of a solid phase extraction column after nitrogen is led, sealing the upper end of the empty column tube of a solid phase extraction column, triggering polymerization at a temperature ranging between 10 DEG C and 30 DEG C for 12 hours to 60 hours, and then obtaining the molecular imprinting monolithic column. The molecular imprinting monolithic column which has good adsorption and enrichment effects on the organic arsines veterinary drug is obtained after the column is eluted from a porogen and the template molecules by a mixed solution of the carbinol and acetic acid. Compared with a traditional mass polymerization method, the method has advantages that the preparation and the postprocessing are simple, recognition is good, cost is low, and the required apparatus and reaction conditions can be acquired easily. The molecular imprinting monolithic column provided by the method can separate, enrich and purify the organic arsines veterinary drug remained in animal origin foods.

Description

technical field [0001] The invention relates to the field of analytical chemistry, in particular to the preparation of an organic arsine molecularly imprinted monolithic column and its application in the analysis of organic arsine veterinary drug residues in animal foods. Background technique [0002] Organic arsines are a class of widely used antibacterial and growth-promoting feed additive drugs. Due to their good effects in improving production performance, they have become one of the most widely used and demanded feed additive drugs in the world. In the 1940s, Fleming Pharmaceutical Company of the United States first successfully developed the organic arsine drug p-aminophenylarsine. In 1993, my country approved the use of this drug for pigs and chickens, and in 1996, it approved the use of roxarsine. . In addition, this type of drug also has a good control effect on various parasites such as histomonas, amoeba, trichomonas, balancidia, eperythrozoon, coccidiosis, piripl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/08
Inventor 李兆周李松彪侯玉泽吕璞张敏刘世磊李志康李文荣
Owner HENAN UNIV OF SCI & TECH
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