Method for screening active ingredients of traditional Chinese medicine by nano mesoporous material immobilized target protein

A technology of nano-mesoporous materials and active ingredients, which is applied in the field of nano-mesoporous materials to immobilize target proteins to screen active ingredients of traditional Chinese medicines. It can solve the problems of easy inactivation of target proteins, low reliability of screening results, and inability to judge the type of inhibition. Low column pressure, high reliability, and adjustable pore size
CN102937631AActive Publication Date: 2013-02-20BEIJING INSTITUTE OF TECHNOLOGYGY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
BEIJING INSTITUTE OF TECHNOLOGYGY
Publication Date
2013-02-20

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Abstract

The invention relates to a method for screening active ingredients of traditional Chinese medicine by nano mesoporous material immobilized target protein. The method includes filling mesoporous material immobilized protein into a chromatographic column, preparing an immobilized target protein inhibitor screening model, dissolving inhibitor having representative inhibiting effect on the immobilized protein into buffer solution to serve as a mobile phase, introducing samples of substrates with saturation concentration every fixed time, collecting peak appearance fraction, replacing the mobile phase with buffer solution free of inhibitor after fixed times, introducing samples of the substrates with saturation concentration every fixed time, respectively freezing and drying the fraction, dissolving the fraction into water, separating the substrates from a product through the analytical type chromatographic column, recording peak area of the product, drawing an inhibition curve of the immobilized target protein inhibitor, checking the model and adopting the prepared model to dissolve aqueous extract of traditional Chinese medicine to be screened into the buffer solution to serve as the mobile phase. Whether the traditional Chinese medicine has inhibition effect or not and the process and the type of the traditional Chinese medicine can be judged according to the peak area of the product. The method has the advantages of high reliability, online performance and the like.
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Description

technical field

[0001] The invention relates to a method for screening active components of traditional Chinese medicine, in particular to a method for screening active components of traditional Chinese medicine by immobilizing target proteins with nanometer mesoporous materials. Background technique

[0002] Drug screening is a step in the process of modern drug development to test and obtain specific physiologically active compounds, and it is the process of selecting compounds with higher activity on a specific target from a large number of compounds or new compounds through standardized experimental methods. Drug targets include genetic sites, receptors, enzymes, ion channels, nucleic acids and other biological macromolecules. Recent studies have shown that enzymes have many advantages as drug targets, and many important research results have been obtained.

[0003] However, traditional Chinese medicine screening using the target protein as the target generally adopts th...

Claims

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