Hepatitis B virus e antibody detection kit

A technology for antibody detection and hepatitis B virus, applied in measuring devices, color/spectral characteristic measurement, instruments, etc., can solve the problems of insufficient detection effect, false positive, false negative, etc., and achieve strong practicability, strong specificity, and easy operation Effect

Inactive Publication Date: 2021-08-13
中山生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the insufficient detection effect of the existing hepatitis B e antibody related detection products on the market, false positives or false negatives are prone to occur

Method used

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  • Hepatitis B virus e antibody detection kit
  • Hepatitis B virus e antibody detection kit
  • Hepatitis B virus e antibody detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] In this embodiment, a detection kit for hepatitis B virus e antibody is prepared, which includes the following components.

[0048] (1) Coated plates coated with hepatitis B virus e antibody (anti-HBe)

[0049] The hepatitis B virus e antibody (anti-HBe) used was purchased from Xiamen Bosheng Biotechnology Co., Ltd. After the antibody was purified by affinity chromatography, two bands were detected by SDS-PAGE or other methods, with a molecular weight of about 25KD, 50KD, and the purified antibody is stored at -20°C or below.

[0050] Coating: After diluting hepatitis B virus e antibody (anti-HBe) with coating buffer, add 100 μL / well to the wells of the microwell plate, absorb at 18-26°C for 20-30 hours, and then tap dry ; Blocking: Use a solution containing 0.121% Tris, 1% BSA, 5% sucrose, and 0.02% thimerosal, with a pH of 8.9-9.1 as the blocking solution, add 150 μL / well to the wells of the microwell plate, and place in 18- Block at 26°C for 16-26h; dry and seal th...

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Abstract

The invention discloses a hepatitis B virus e antibody detection kit. The kit comprises a coated plate coated with a hepatitis B virus e antibody, a substrate solution and an enzyme labeled anti-HBe solution. The prepared kit for detecting the antibody has the effects of high specificity, good repeatability and high sensitivity.

Description

technical field [0001] The invention belongs to the technical field of in vitro diagnosis and detection, and in particular relates to a detection kit for hepatitis B virus e antibody. Background technique [0002] Hepatitis B virus (hepatitis B virus, HBV) is the pathogen that causes hepatitis B in humans and is one of the most widely transmitted deadly viruses in the world. The infection of hepatitis B virus has become one of the most important public health problems in the world. Since there is no effective means to treat hepatitis B at present, vaccination and early detection and diagnosis are the focus of prevention of hepatitis B. [0003] The etiological diagnosis of hepatitis B, the most commonly used is the detection of hepatitis B virus serum markers (HBVM). HBVM mainly includes seven serum markers: hepatitis B surface antigen, hepatitis B surface antibody, hepatitis B core antibody, hepatitis B e antigen, hepatitis B e antibody, HBV-DNA and DNA polymerase. By det...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/576G01N33/569G01N33/535G01N33/543G01N21/76G01N21/31
CPCG01N21/31G01N21/76G01N33/535G01N33/54306G01N33/56983G01N33/5761
Inventor 王胜岚范洁云黄永宏李梅
Owner 中山生物工程有限公司
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