Iron-enriched cordyceps militaris liquid deep fermentation culture medium and culture method thereof

A liquid submerged fermentation and fermentation culture technology, applied in the direction of fungi, etc., to achieve the effects of high yield, increased nutritional value, and easy access to raw materials

Active Publication Date: 2014-11-05
NANTONG INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] There is a great demand in this field for the development of additional functional products of Cordyceps militaris, but iron-rich Cordyceps militaris has not been reported so far

Method used

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  • Iron-enriched cordyceps militaris liquid deep fermentation culture medium and culture method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0033] The liquid submerged fermentation culture of embodiment 1 iron-rich Cordyceps militaris

[0034] (1) Strain activation:

[0035] Prepare PDA medium: potato 200g / L, glucose 20g / L, agar powder 20g / L, KH 2 PO 4 1.5g / L, MgSO 4 ·7H2O1.5g / L, thiamine 50×10 -6 g / L; its pH value is 6, and it can be sterilized at 121°C for 20 minutes.

[0036] Inoculate the strain of Cordyceps militaris on the slant of the test tube under aseptic conditions, and cultivate it in an incubator at 26° C. for 5 to 7 days.

[0037] (2) Domestication of iron-resistant Cordyceps militaris strains:

[0038] Prepare PDA optimized medium: potato 200g / L, glucose 20g / L, peptone 20g / L, agar powder 20g / L, KH 2 PO 4 1.5g / L, MgSO 4 ·7H 2 O1.5g / L, different concentrations of Fe 2+ (7H 2 O·FeSO4) solution, thiamine 50×10 -6 g / L, agar 25g / L; its Ph value is 6, sterilized at 121°C for 20min.

[0039] The slant bacteria were inoculated on the plate medium for acclimatization culture, and placed in an in...

Embodiment 2

[0056] Example 2 Liquid submerged fermentation culture of iron-rich Cordyceps militaris under the condition of 0.06mg / ml iron ion concentration

[0057] According to the liquid submerged fermentation culture method of iron-rich Cordyceps militaris in embodiment 1, Fe 2+ The solution uses 0.06mg / ml FeSO 4 ·7H 2 O solution, the fermentation culture time adopts 84 hours, obtains iron-enriched Cordyceps militaris.

[0058] The biomass of the above-mentioned iron-rich Cordyceps militaris was measured to be 18.930g / L, the enriched iron content was 16.011mg / g, the content of adenosine was 2314.6μg / g, cordycepin was 900.21μg / g, and Cordyceps polysaccharide was 52.37mg / g. g, the content of cordycepic acid is 35.84mg / g.

[0059] In addition, the contents of adenosine, cordycepin, cordyceps polysaccharide, and cordycepic acid in the common Cordyceps militaris obtained in Experiment 1 in Example 1 were measured to be 385.1 μg / g, 51.05 μg / g, 32.51 mg / g, and 34.31 mg / g, respectively. g....

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Abstract

The invention provides an iron-enriched cordyceps militaris liquid deep fermentation culture medium and a culture method thereof. The culture medium comprises the components and the contents: 10-45 g / L of a soybean powder, 10-45 g / L of sucrose, 0-1.5 g / L of KH2PO4, 0-1.5 g / L of MgSO4, 50*10<-6> g / L of thiamine, and 0.04-0.06 g / L of an Fe<2+> solution, wherein the pH value is 6.0. The culture method comprises the steps: strain activation, iron-resistant cordyceps militaris strain domestication, seed enlargement culture, iron-enriched cordyceps militaris fermentation, and drying separation. The culture method has the advantages of simple operation and short culture period, and enables a cordyceps militaris product to play a role in supplementing a trace element iron in addition to exerting pharmacological effects of cordyceps militaris.

Description

technical field [0001] The invention relates to a liquid submerged fermentation culture method of Cordyceps militaris, in particular to a liquid submerged fermentation culture method of iron-rich Cordyceps militaris. Background technique [0002] Cordyceps militaris is also known as Cordyceps militaris, Cordyceps militaris, etc. Studies have confirmed that the main medicinal ingredients of Cordyceps militaris are nucleoside compounds, sugar alcohols, polysaccharides, amino acids, enzymes, and mineral elements, which are similar to the medicinal ingredients of Cordyceps sinensis. Therefore, Cordyceps militaris has similar effects to Cordyceps sinensis. The physiological functions of Cordyceps militaris include: improving immunity, anti-virus and antibacterial effects, anti-fatigue, anti-tumor, sedative and anti-convulsant effects, anti-radiation, lowering blood sugar, lowering blood fat, delaying aging, etc. With the progress of society and the development of science and te...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14
Inventor 李慧英
Owner NANTONG INST OF TECH
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