A detection method for determining the residual amount of l900 washing liquid

A detection method and residual amount technology, which is applied in the detection field of determining the residual amount of L900 washing liquid in the cleaning of plastic laboratory utensils and glassware, can solve the problems of low detection sensitivity and inability to meet the quality control of medicines, and achieve high sensitivity and ensure specificity performance, sensitivity enhancement effect

Active Publication Date: 2016-06-29
ZHEJIANG PUKANG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Sodium octyl sulfate and sodium octyl sulfonate are used as anionic surfactants, which are often determined by methylene blue spectrophotometry (GB7494-87), but the existing methods have low sensitivity and are easily interfered by other chemical substances , so that it cannot meet the requirements of drug quality control

Method used

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  • A detection method for determining the residual amount of l900 washing liquid
  • A detection method for determining the residual amount of l900 washing liquid
  • A detection method for determining the residual amount of l900 washing liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The detection of residual amount of L900 detergent in the plastic pharmaceutical utensils of embodiment 1

[0028] 1 Agilent PlexaPAX anion exchange column enrichment sample

[0029] (1) Use ultrapure water to prepare series concentration standard solutions of L900 detergent, taking sodium octyl sulfate and sodium octyl sulfonate as units, and prepare the concentrations as follows: 0, 2ng / ml, 10ng / ml, 20ng / ml , 100ng / ml, 1000ng / ml, prepare 1000ml for each dilution concentration;

[0030] (2) Add 15 ml of ammonia water to each diluted concentration standard and sample to be tested and mix well for pretreatment;

[0031] (3) Take the Agilent PlexaPAX anion exchange column and elute it first with 2 ml of ethanol, then with 2 ml of ultrapure water; then use a peristaltic pump to pass the pretreated sample through the activated Agilent PlexaPAX anion exchange column, and use each sample separately A PlexaPAX anion exchange column;

[0032] (4) Wash the exchange column wit...

Embodiment 2

[0052] Accuracy and sensitivity analysis of embodiment 2 detection method

[0053] By analyzing the recovery rate of standard solutions of various concentrations, the results show that the linear correlation coefficient R2 of the obtained linear regression equation is 1, and the concentration recovery rate of each standard solution is 93.86%-117.37%, indicating that the detection method is in the range of 2-1000ng / ml Has better accuracy.

[0054] And at the concentration of 2ng / ml, the recovery rate can reach 117.37%, indicating that the sensitivity of this detection method can reach 2ng / ml.

Embodiment 3

[0055] Embodiment 3 specificity analysis detection method

[0056] The present invention determines the residue of the L900 cleaning solution by sodium octyl sulfate and sodium octyl sulfonate, so the sodium octyl sulfate standard product, sodium octyl sulfonate standard product and L900 cleaning solution of the same concentration are in the liquid Comparing the retention times of the three phases, the retention times and peak areas of the three were basically consistent, and the coefficients of variation (CV) were 0.01% and 2.78%, respectively. Using this method to determine the content of sodium octyl sulfate and sodium octyl sulfonate in L900 washing liquid has good specificity. (table 3, figure 2 , image 3 , Figure 4 )

[0057] Table 3 Retention time of standard substance and L900 washing solution in liquid phase

[0058]

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Abstract

The invention relates to the technical field of chemical composition test, and in particular relates to a method for detecting the residual amount of an L900 washing liquid in the cleaning process of plastic experimental apparatuses and glass wares of pharmacy, medical research, academics, biotechnology and other institutions. The method is used for carrying out quantitative analysis on residual amount of the L900 washing liquid in the plastic experimental apparatuses and the glass wares by combining an anion exchange column and reversed-phase high-performance liquid chromatography based on a methylene blue spectrophotometric method; octyl sodium sulfate and octyl sodium sulfonate in the washing liquid of the plastic experimental apparatuses and the glass wares are enriched and concentrated by simple and efficient steps, so that the detection sensitivity is improved; reversed-phase high-performance liquid chromatography is used for effectively separating out the octyl sodium sulfate and the octyl sodium sulfonate which are combined with methylene blue, so that the interference of other substances for detection can be eliminated, and the specificity of the method is effectively guaranteed. The method has the detection limit of 2ng / mL for the octyl sodium sulfate and the octyl sodium sulfonate, so that the accuracy of the detected data is effectively guaranteed, and the sample detection of the L900 washing liquid can be better carried out at the very low concentration.

Description

technical field [0001] The invention relates to the technical field of chemical component detection, in particular to a detection method for measuring the residual amount of L900 washing liquid in the cleaning of plastic laboratory utensils and glassware used in pharmacy, medical research, schools, biotechnology and other institutions. Background technique [0002] L900 Liquid Detergent is produced by Nalgene and is a liquid detergent specially used for cleaning plastic utensils in pharmaceutical, medical research, schools, biotechnology and other institutions. This cleaning solution is suitable for use on all plastics, especially polycarbonate, and will not cause cracks, stress cracks or stains. Nalgene is currently one of the largest suppliers of laboratory and medical containers in the United States. The medical products produced by the company are popular among pharmaceutical companies in the United States and China because of their firmness, hygiene and ease of use. In...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/89
Inventor 朱赟高孟毛岱敏方任华田寅忻亚娟庄昉成钱汶毛子安毛江森
Owner ZHEJIANG PUKANG BIOTECH
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