A hplc detection method for mes, nhs residues
A detection method, the technology of morpholineethanesulfonic acid, applied in the field of HPLC detection, can solve the problems of single quantitative increase and difficulty, and achieve the effect of good linear range, high accuracy, and simple and easy operation method
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[0028] 1. Device information
[0029] Chromatograph: Shimadzu high performance liquid chromatograph LC-20AB;
[0030] Chromatographic column: ZORBAX.SB-C184.6mm*250mm*5μm;
[0031] Injection volume: 10 μL.
[0032] 2. Investigation of MES and NHS detection methods by high performance liquid chromatography
[0033] 2.1 Preparation of MES and NHS test solution
[0034] MES test solution (300μg / mL): Accurately weigh 30.09mg of the sample, dissolve it in purified water, and dilute to 100mL.
[0035] NHS test solution (300μg / mL): Accurately weigh 30.07mg of the sample, dissolve it in purified water, and dilute to 100mL.
[0036] 2.2 Determination of detection wavelength
[0037] The test solutions of MES and NHS were taken respectively and scanned at full wavelength on the ultraviolet-visible spectrophotometer. The results show that NHS absorbs from 300nm and reaches the maximum at 200nm, MES absorbs from 220nm and reaches the maximum at 200nm, because the organic phase of th...
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