Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A hplc detection method for mes, nhs residues

A detection method, the technology of morpholineethanesulfonic acid, applied in the field of HPLC detection, can solve the problems of single quantitative increase and difficulty, and achieve the effect of good linear range, high accuracy, and simple and easy operation method

Active Publication Date: 2016-02-24
ZHEJIANG JINGJIA MEDICAL TECH CO LTD
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Two substances, 2-morpholineethanesulfonic acid (MES) and N-hydroxysuccinimide (NHS), are more polar, and their solubility in water is greater, which actually achieves a separate quantitative increase for the two substances Difficulty

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A hplc detection method for mes, nhs residues
  • A hplc detection method for mes, nhs residues
  • A hplc detection method for mes, nhs residues

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0028] 1. Device information

[0029] Chromatograph: Shimadzu high performance liquid chromatograph LC-20AB;

[0030] Chromatographic column: ZORBAX.SB-C184.6mm*250mm*5μm;

[0031] Injection volume: 10 μL.

[0032] 2. Investigation of MES and NHS detection methods by high performance liquid chromatography

[0033] 2.1 Preparation of MES and NHS test solution

[0034] MES test solution (300μg / mL): Accurately weigh 30.09mg of the sample, dissolve it in purified water, and dilute to 100mL.

[0035] NHS test solution (300μg / mL): Accurately weigh 30.07mg of the sample, dissolve it in purified water, and dilute to 100mL.

[0036] 2.2 Determination of detection wavelength

[0037] The test solutions of MES and NHS were taken respectively and scanned at full wavelength on the ultraviolet-visible spectrophotometer. The results show that NHS absorbs from 300nm and reaches the maximum at 200nm, MES absorbs from 220nm and reaches the maximum at 200nm, because the organic phase of th...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
lengthaaaaaaaaaa
particle diameteraaaaaaaaaa
wavelengthaaaaaaaaaa
Login to View More

Abstract

The invention discloses an HPLC detection method of MES and NHS residues. A reversed-phase C18 silica bonded chromatographic column is used, a mobile phase consists of a phosphate buffer of pH 6.5 to 6.8, and an organic solvent, and the detection wavelength is 205 to 210nm; the organic solvent is methanol or acetonitrile. According to the method, samples do not need to be specially treated, the operation method is simple and easy to implement, the accuracy is high, the needs in actual detection can be met, and two substances MES and NHS can be detected to reach microgram scale.

Description

technical field [0001] The invention relates to an HPLC detection method for MES and NHS residues. Background technique [0002] Among the relatively stable chemical cross-linking methods nowadays, the cross-linking system of carbodiimide has attracted more and more attention. The cross-linking system of carbodiimide can be applied to biological polysaccharides, polypeptides, proteins, nucleic acids and polymer modification, etc. In various fields of biology and organic synthesis, its own advantages such as low toxicity, rapid cross-linking effect, and stable results have excellently made up for the safety problems caused by the traditional use of formaldehyde and glutaraldehyde cross-linking methods. Compared with physical cross-linking methods such as thermal cross-linking, the cross-linking system of carbodiimide is more stable, and the cross-linked products are more uniform. 2-Morpholineethanesulfonic acid (MES) provides a relatively stable pH range in the cross-linking...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/74
Inventor 杨中科龚彦铭陈卫英丁祥李娟李好雨张兆利杨秀甫彭红卫孙莉嫚杨安顺蒋小龙
Owner ZHEJIANG JINGJIA MEDICAL TECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com