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Qinglongbaihu soup and quality detection method thereof

A technology of Baihu and Qinglong, which is applied in the field of quality control of traditional Chinese medicine, to achieve good peak shape, good peak separation, and save time and cost

Active Publication Date: 2021-03-16
CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the current lack of quality testing methods for the decoction, decoction pieces, intermediates and material references of the prescription Qinglongbaihu Decoction, the purpose of the present invention is to provide a Qinglongbaihu decoction with a standardized HPLC fingerprint soup, and provide a more accurate quality detection method for Qinglong Baihu Decoction

Method used

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  • Qinglongbaihu soup and quality detection method thereof
  • Qinglongbaihu soup and quality detection method thereof
  • Qinglongbaihu soup and quality detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1: Detection of Qinglong Baihu Decoction Standard Fingerprint

[0061] 1. Preparation of standard solution

[0062] Weigh 60g of green fruit decoction pieces into a beaker, add 8 times the amount of water to soak for 30 minutes, decoct twice, each time for 1 hour, combine the two green fruit water decoctions, and obtain. Weigh 100 g of radish cut into small pieces, add 3 times the amount of water and decoct for 60 minutes to obtain radish decoction. Take 300ml of green fruit water decoction and 100ml of radish water decoction and combine, and decoct until 300mL to obtain Qinglongbaihutang decoction. Concentrate 300ml of the decoction solution to about 100ml under reduced pressure to obtain a concentrated solution; freeze-dry the concentrated solution to obtain the substance standard of Qinglongbaihutang.

[0063] Said green fruit water decoction, radish water decoction, Qinglongbaihu decoction decoction, Qinglongbaihu decoction concentrate and the substance re...

Embodiment 2

[0080] Embodiment 2: the quality detection method of Qinglong Baihu Decoction

[0081] 1, the preparation of need testing solution: identical with the method for preparing standard solution among the embodiment 1.

[0082] 2. Preparation of reference solution:

[0083] Weigh an appropriate amount of reference substances of gallic acid, protocatechuic acid, chlorogenic acid, epicatechin, scopoletin, and ellagic acid, weigh them accurately, and add methanol to make each 1ml contain gallic acid and protocatechuic acid respectively , chlorogenic acid, epicatechin, scopoletin, ellagic acid 0.943mg, 0.757mg, 1.025mg, 1.160mg, 1.080mg, 1.032mg of the reference substance stock solution I, and then each measure 0.5ml to 5ml Mix well in the volumetric flask, dilute to volume with methanol to obtain mixed reference substance stock solution II. The stock solution was stored in a refrigerator.

[0084] 3. High performance liquid chromatography detection:

[0085] Adopt the chromatograp...

experiment example 1

[0102] Experimental Example 1: Investigation of Chromatographic Conditions

[0103] 1) Investigation of mobile phase system

[0104] Preliminary inspection methanol (A)-0.1% phosphoric acid aqueous solution (B), acetonitrile (A)-0.1% phosphoric acid aqueous solution (B), acetonitrile (A)-0.5% phosphoric acid aqueous solution (B), acetonitrile (A)-water (B) The analysis of these four mobile phase samples, such as Figure 7-10 As shown, when the result analysis obtained that the mobile phase was acetonitrile (A)-0.1% phosphoric acid aqueous solution (B), the sample chromatographic peak shape, resolution, and number of theoretical plates were all better; so the selected mobile phase was acetonitrile (A)- 0.1% phosphoric acid aqueous solution (B).

[0105] 2) Inspection of column temperature

[0106] Investigate the analysis of samples under three different column temperature conditions of 25°C, 30°C, and 35°C, such as Figure 11-13 As shown, the results show that there is no ...

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Abstract

The invention belongs to the technical field of traditional Chinese medicine quality control, and particularly relates to Qinglongbaihu soup and a quality detection method thereof. Aiming at the problem that a method for detecting the quality of water decoction, decoction pieces, intermediates and material references of the formula Qinglongbaihu soup does not exist at present, the technical schemeof the invention provides the Qinglongbaihu soup with a standardized high performance liquid chromatography fingerprint spectrum, and provides a more accurate quality detection method of the Qinglongbaihu soup. According to the method, the standard fingerprint spectrum of the Qinglongbaihu soup can be obtained, whether the Qinglongbaihu soup test sample is a qualified product or not can be accurately and stably judged, and the content of effective components in the Qinglongbaihu soup can be accurately determined.

Description

technical field [0001] The invention belongs to the technical field of quality control of traditional Chinese medicines, and in particular relates to Qinglong Baihu Decoction and a quality detection method thereof. Background technique [0002] Qinglong Baihu Decoction (Qinglong Baihu Decoction) comes from the second volume of "Wang's Medical Cases" by Wang Mengying, a famous physician in the Qing Dynasty. This prescription is a dual-purpose prescription for medicine and food, which is highly in line with the essence of the theory of "medicine and food are of the same origin" that has existed in traditional Chinese medicine since ancient times. It embodies the traditional characteristics of traditional Chinese medicine, and is widely used with its advantages of "very simple medicine, mildest nature, not bad taste, easy to powder and easy to drink". In the prescription, "the green fruit is green in color, and the radish is white in color. When combined into a medicine, it ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/86
CPCG01N30/02G01N30/06G01N30/8679G01N30/8686
Inventor 廖婉傅超美郑勇凤李锐游宇陈姣朱宗萍谭瑾傅舒董芹杨青松王继森王准
Owner CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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