Method for detection of modified nucleosides in urine
A technology for modifying nucleosides and detection methods, which is applied in measuring devices, instruments, scientific instruments, etc., can solve problems such as pollution, and achieve the effects of avoiding pollution, good stability, and improved detection range
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[0044] Instruments and materials:
[0045] American Agilent 6495 tandem mass spectrometer, Angilent 1290 liquid chromatograph, the chromatographic column is Agilent C18 column, the specification is 50mm×3.0mm, 2.7μm.
[0046] Drugs and reagents:
[0047] Standard: pseudouridine, cytidine, 1-methyladenosine, uridine, adenosine, inosine, guanosine, 1-methylhypoxanthine Glycoside, 5-methyluridine, xanthine nucleoside, 3-methyluridine, 1-methylguanosine, 6-methyladenosine, 2-methylguanosine, N4-acetylcytidine, N2 , N2-dimethylguanosine were purchased from Sigma-Aldrich Company.
[0048]Internal standard: 8-bromoguanosine (8-Br-G), purchased from Sigma-Aldrich Company.
[0049] Methanol: chromatographically pure, Merck Company.
[0050] Formic acid: chromatography pure, Merck company.
[0051] Ultrapure water: Thermo Corporation.
[0052] Urine sample: volunteer urine sample.
[0053] Accurately weigh the above-mentioned 16 kinds of standard substances, dissolve them in meth...
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