Flexible micro-column ring array for detecting clot contractility and preparation method and application thereof

A contractile force and micro-column technology, applied in the field of biomedicine, can solve the problems of difficult clinical transformation, complex device processing, and high inspection costs, and achieve the effects of objective and comprehensive test results, high sensitivity, and low inspection costs.

Active Publication Date: 2020-08-18
SHENZHEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, the above-mentioned methods are complicated to process and require extremely sophisticated inspection platforms and complex data acquisition equipment, such as fluorescence microscopes and atomic force microscopes; the high cost of inspection makes it only suitable for mechanistic research, and it is difficult for clinical transformation
In addition, the above method needs to separate platelets from blood, which complicates the operation

Method used

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  • Flexible micro-column ring array for detecting clot contractility and preparation method and application thereof
  • Flexible micro-column ring array for detecting clot contractility and preparation method and application thereof
  • Flexible micro-column ring array for detecting clot contractility and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] Example 1 A flexible microcolumn ring array and its chip for detecting clot contraction force

[0073] Such as image 3 As shown, a flexible microcolumn ring array for detecting clot contraction force, wherein the microcolumn ring array is composed of several microcolumn rows arranged periodically, and each microcolumn row has several microcolumn rings , each micro-column ring is composed of four ring-shaped micro-columns; the micro-column ring has a circular hole with a certain diameter. In-house coagulation test. The diameter of the micro-column ring is 1 mm, and the distance between each micro-column ring is 1 mm; the column height of the micro-column is 1.2 mm, and the diameter of the upper surface of the column is 0.2 mm. The microcolumn ring array is a circular array; the microcolumn is cylindrical.

[0074] In addition, if image 3 As shown in (b) figure, this embodiment also provides a flexible microcolumn ring array chip for detecting clot contraction force...

Embodiment 2

[0075] Example 2 A flexible microcolumn ring array and its chip for detecting clot contraction force

[0076] Such as image 3 As shown, a flexible microcolumn ring array for detecting clot contraction force, wherein the microcolumn ring array is composed of several microcolumn rows arranged periodically, and each microcolumn row has several microcolumn rings , each micro-column ring is composed of four ring-shaped micro-columns; the micro-column ring has a circular hole with a certain diameter. In-house coagulation test. The diameter of the micro-column ring is 3 mm, and the distance between each micro-column ring is 5 mm; the column height of the micro-column is 1.6 mm, and the diameter of the upper surface of the column is 0.4 mm. The microcolumn ring array is a circular array, an elliptical array or a polygonal array; the microcolumn is in the form of a cylinder, an elliptical cylinder or a polygonal cylinder.

[0077] In addition, if image 3As shown in (b) figure, th...

Embodiment 3

[0079] Example 3 A flexible microcolumn ring array and its chip preparation method for detecting clot contractility

[0080] A method for preparing the flexible microcolumn ring array chip for detecting clot contraction force, comprising the following steps:

[0081] S1. Use Comsol simulation software to simulate the scene of micro-column bending under force, process and design the micro-column, determine the feasible size and actual spring coefficient of the micro-column, and the relevant parameters of the PDMS material;

[0082] S2. According to the design result of S1, a mold is obtained by cutting an acrylic plate with a laser, and finally the flexible microcolumn ring array chip for detecting clot contraction force is fabricated by reverse molding using PDMS.

[0083] The invention utilizes laser engraving and flexible material PDMS to process and obtain the microcolumn ring array or the microcolumn ring array chip. Specifically:

[0084] 1. Theoretical simulation

[0...

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Abstract

The invention discloses a flexible micro-column ring array for detecting the contractility of a clot and a preparation method and application of the flexible micro-column ring array. The micro-columnring array is composed of a plurality of micro-column rows arranged periodically, each micro-column row is provided with a plurality of micro-column rings, and each micro-column ring is composed of four or more micro-columns forming a ring shape in a surrounding mode, wherein a circular pore with a certain diameter is formed in the micro-column ring, and when the contractility of the clot is detected, whole blood is dropped into the circular pore of the micro-column ring for blood coagulation detection. According to the invention, a whole blood sample is efficiently fixed in the micro-column ring by utilizing the interfacial tension of a solid-liquid-gas system and the resistance of the micro-column; meanwhile, the micro-column is used as a force sensor to measure the contraction force ofa clot in real time, the detection result is high in sensitivity and low in detection cost, and clinical transformation is easy; besides, platelets do not need to be separated from blood, whole bloodcan be used for detection, the influence of other components in the blood on platelet contraction is fully considered, and the result is more objective and comprehensive.

Description

technical field [0001] The invention belongs to the technical field of biomedicine. Specifically, it relates to a flexible microcolumn ring array for detecting clot contraction force, its preparation method and application. Background technique [0002] Blood coagulation is a very important physiological process, which must be rationally regulated by the body to maintain the delicate balance between bleeding and thrombus formation. Insufficient coagulation function can lead to excessive blood loss in patients with trauma, and excessive coagulation function is often accompanied by the risk of thrombosis. Coagulation function tests play an important role in clinical practice. During cardiopulmonary bypass, surgeons need to detect the coagulation function of blood in real time to guide the administration of coagulants and anticoagulants to prevent blood coagulation during extracorporeal circulation[1]. In addition, surgery-induced blood loss, contact coagulation activation, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/49B23K26/38B23K26/402
CPCG01N33/49G01N33/4905B23K26/38B23K26/402
Inventor 李自达黄兰蛛郑卫东
Owner SHENZHEN UNIV
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