Regeneration culture medium and culture method for improving regenerated adventitious buds of echinacea explants

A regeneration medium, the technology of Echinacea purpurea, applied in the field of plant biology, can solve the problems of low regeneration rate and poor quality of regenerated buds

Active Publication Date: 2014-01-01
广州市泰丰源实业有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the defects of low regeneration rate and poor quality of regenerated buds when echinacea explants regenerate adventitious buds in the prior art, the present invention provides a regeneration medium for improving echinacea purpurea explants to regenerate adventitious buds

Method used

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  • Regeneration culture medium and culture method for improving regenerated adventitious buds of echinacea explants
  • Regeneration culture medium and culture method for improving regenerated adventitious buds of echinacea explants
  • Regeneration culture medium and culture method for improving regenerated adventitious buds of echinacea explants

Examples

Experimental program
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Effect test

Embodiment 1

[0028] S1. Explant preparation: several leaves, petioles and roots were cut from the intact Echinacea purpurea seedlings cultured with scissors. The leaves, petioles and roots of different ploidy come from tissue-cultured intact sterile seedlings of different ploidy. After rinsing the collected leaves, petioles and roots with tap water for 30 min, put all the leaves, petioles and roots in a glass beaker with a capacity of 1000 mL on the ultra-clean bench, add 75% ethanol solution to the beaker until submerged After 1 min, pour off the ethanol solution to keep the blades, petioles and roots; then add 2% sodium hypochlorite solution to the beaker until all the leaves, petioles and roots are submerged. After 20 min, Pour off the sodium hypochlorite solution, keep the leaves, petioles and roots; then add sterile distilled water to the beaker until all the leaves, petioles and roots are submerged, rinse the leaves, petioles and roots for 2 minutes, pour off the distilled water, keep ...

Embodiment 2

[0037] S1. Preparation of explants: same as in Example 1.

[0038] In S2, triploid Echinacea purpurea leaves, petioles and root explants were inoculated on regeneration medium containing different concentrations of DA-6 (0, 0.01, 0.08, 0.16, 0.32 mg / L) (the regeneration medium was prepared in MS Add 30 g / L sucrose, 4.5 g / L agar, 0.3 mg / L BA and 0.01 mg / L NAA to the medium). Test adventitious bud-induced regeneration. The preparation method of the regeneration medium containing different concentrations of DA-6 (0, 0.01, 0.08, 0.16, 0.32 mg / L) is the same as that in Example 1. The culture conditions for adventitious bud-induced regeneration are to inoculate different types of Echinacea purpurea explants in their respective appropriate regeneration medium, at a culture temperature of 25±4°C, with a light intensity of 1000-2500 lx and a light time of 10-16 h Cultivate for 35 days under the condition of / d.

[0039] The results are shown in Table 2 and figure 2 . From Ta...

Embodiment 3

[0044] S1. Preparation of explants: same as in Example 1.

[0045] S2 Tetraploid Echinacea purpurea leaf, petiole and root explants were inoculated on regeneration medium containing different concentrations of DA-6 (0, 0.01, 0.08, 0.16, 0.32 mg / L) (the regeneration medium was prepared in MS Add 30 g / L sucrose, 4.5 g / L agar, 0.3 mg / L BA and 0.01 mg / L NAA to the medium). Test adventitious bud-induced regeneration. The preparation method of the regeneration medium containing different concentrations of DA-6 (0, 0.01, 0.08, 0.16, 0.32 mg / L) is the same as that in Example 1. The culture conditions for adventitious bud-induced regeneration are to inoculate different types of Echinacea purpurea explants in their respective appropriate regeneration medium, at a culture temperature of 25±4°C, with a light intensity of 1000-2500 lx and a light time of 10-16 h / d for 35 d.

[0046] The results are shown in Table 3 and image 3 . from Table 3 and image 3 It can be seen that DA-...

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Abstract

The invention belongs to the technical field of plant biology, and particularly discloses a regeneration culture medium and a culture method for improving regenerated adventitious buds of echinacea explants. According to the regeneration culture medium, corresponding concentration of DA-6 (diethyl aminoethyl hexanoate) is also especially added besides that 15-60g/L of cane sugar, 3-9g/L of agar, 0.1-1.5mg/L of BA (benzylaminopurine) and 0.01-0.2mg/L of NAA (naphthaleneacetic acid) are added to an MS (Murashige and Skoog) formula aiming at difference of different ploidies and different sources of echinacea explants on the sensitivity to diethyl aminoethyl hexanoate (DA-6). By adopting the regeneration culture medium, the effect of facilitating regeneration of BA-induced adventitious buds is significantly enhanced by adding DA-6. By adopting the regeneration culture medium and the culture method disclosed by the invention, the problem of different genotype limits in regeneration cultivation of echinacea can be effectively overcome; the regeneration efficiency of the adventitious buds is greatly improved; development of relevant research work of biotechnology breeding of the echinacea is facilitated.

Description

technical field [0001] The invention relates to the field of plant biotechnology, in particular to a regeneration medium and a cultivation method for improving the regeneration of adventitious buds from echinacea purpurea explants. Background technique [0002] Echinacea ( Echinacea purpurea L.) is a perennial herb of Compositae, which has a good immune regulation effect, and its extract is an immune enhancer and immune regulator that has received widespread attention in the world in recent years. Echinacea has become a world-renowned medicinal plant due to its precise efficacy. In order to obtain various varieties of Echinacea purpurea, people have tried to apply a variety of biotechnological means to it, such as transgenic, anther culture, and chromosome doubling. The achievement of these goals must go through a common step, that is, to induce the culture to produce adventitious buds. Although many studies have been carried out on the regeneration of adventitious buds ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 陈晓鹭杨跃生吴鸿李栋梁
Owner 广州市泰丰源实业有限公司
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