Method for culturing rhododendron fortunei polyploidy

A breeding method, polyploidy technology, applied in the field of plant biology, can solve the problems that the use of Rhododendron versicolor has not been reported yet, and achieve the effect of reducing the cost of cultivation

Active Publication Date: 2018-12-07
ZHEJIANG FORESTRY UNIVERSITY
View PDF2 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Polyploidy breeding has become one of the important means of cultivating new varieties of flowers, and has been s

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for culturing rhododendron fortunei polyploidy
  • Method for culturing rhododendron fortunei polyploidy
  • Method for culturing rhododendron fortunei polyploidy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1: Take the rhododendron brocade in Tiantai Huading Mountain as an example.

[0034]Take 0.1g of rhododendron seeds from Tiantai Huading Mountain for sterilization. The sterilization process is as follows: wash once with sterile water, add 10 mL of 75% alcohol to soak for 30 seconds; pour off the alcohol and wash with sterile water for 3 times, add 10 mL of carbendazim solution (diluted 1000 times) soak for 20min; pour off the carbendazim solution and wash with sterile water 4 times, add 10mL 10% anteformin solution to sterilize for 10min; pour off the anteformin solution and wash with sterile water for 4 times.

[0035] Take the sterilized seeds at 5 mg·L -1 Soak in amisulfame solution for 14 hours, then wash with sterile water for 3 times, sow evenly in a glass petri dish with sterile filter paper, add sterile water to moisten, seal the petri dish with medical tape, and germinate in a light incubator. The germination conditions are temperature 24°C, light ...

Embodiment 2

[0039] Embodiment 2: Take the rhododendron brocade in Kunming, Yunnan as an example.

[0040] Except for the following differences, other steps and methods are the same as in Example 1.

[0041] Take 0.2g of rhododendron rhododendron seeds from Kunming, Yunnan, and use 10mg·L -1 Soak in amisulfame solution for 24 hours, germinate for 20 days, and transplant to seedling blocks. After cultivation and ploidy detection, the polyploid plants are transplanted to an outdoor shade shed for further cultivation.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Login to view more

Abstract

The invention provides a method for culturing rhododendron fortunei polyploidy, and relates to the technical field of plant biology. The method comprises the following steps: sterilizing rhododendronfortunei seeds with oryzalin; transplanting the seeds to a seedling clod for culturing after the seeds are germinated, and detecting the ploidy variation of chromosome by a porosity method and a flowcytometer method; and transplanting the chromosome doubling plant determined by detection into greenhouse for culturing to obtain the polyploidy plant. The rhododendron fortunei polyploidy cultured byadopting the method has high inductivity reaching 30 percent, and high survival rate of variant plants reaching 85 percent or more; and the identifying method is simple and convenient, and can simultaneously prepare massive polyploidy materials, and the breeding efficiency is greatly improved.

Description

technical field [0001] The invention relates to the field of plant biotechnology, in particular to a method for cultivating polyploid Rhododendron versicolor. Background technique [0002] Rhododendron yunjin is a large evergreen shrub. It is a unique Rhododendron plant in China. It grows on hillsides, valleys or rocky mountains at an altitude of 600-2300m. It is widely distributed in Zhejiang, Jiangxi, Anhui, Hunan and other southern provinces. Rhododendron yunjin has beautiful flowers and bright colors. The roots, leaves and flowers can be used as medicine, which has very important ornamental value and medicinal value. There are abundant wild resources of rhododendron brocade in my country, but there are few species, especially those with strong resistance. Cultivating fine new varieties is of great significance to the development and utilization of Rhododendron yunnanensis resources and to enhance its market value. Compared with diploids, polyploids often have the advan...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01H1/08
CPCA01H1/08
Inventor 林二培杨彬楼雄珍牛明月黄华宏童再康
Owner ZHEJIANG FORESTRY UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap