Rice salt-tolerant gene OsRR22 mutant, encoded amino acid sequence thereof, plant and making method of mutant

Abstract

The invention relates to a rice salt-tolerant gene OsRR22 mutant, an encoded amino acid sequence thereof, a plant and a making method of the mutant, and belongs to the technical field of plant biology. A CRISPR/Cas9 technology is sued to edit self-selected rice variety WDR58 with rice salt-tolerant gene OsRR22 to obtain a rice salt-tolerant gene OsRR22 function deleted mutant new germplasm WDR58-cas-1 with important application values. The mutant can significantly increase the salt tolerance of rice, and can be applied to high-yielding salt-tolerant rice breeding.

Description

technical field [0001] The invention belongs to the field of plant biotechnology, and in particular relates to a rice salt-tolerant gene OsRR22 mutant, its coded amino acid sequence, a plant and a method for creating the mutant. Background technique [0002] Rice (Oryza sativa L.) is one of the three major food crops in the world, and it is the ration of 2.2 billion people in the world, including more than 65% of the population in my country. Sufficient supply of rice production directly affects global food security. Rice production faces various abiotic stresses, such as drought, salt damage, chilling damage, high temperature, etc. Rice abiotic stress has become one of the important problems facing agriculture in the 21st century. [0003] Rice is an extremely sensitive crop to salt damage, and salt damage has become the main abiotic stress factor affecting rice growth. At present, more than 6% of the world's land shows salinization; 19.5% of the paddy fields and 2.1% of...

AI Technical Summary

Problems solved by technology

However, there are few reports on the targeted editing of key genes such as yield, resistance, and fertility that are of great value in rice breeding, and there are even fewer reports on gene editing and improvement of excellent rice parents

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