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Technology for obtaining peach embryo aseptic seedlings on large scale

A technology for embryo culture and aseptic seedlings, which is applied in the field of large-scale acquisition of peach embryos to cultivate aseptic seedlings, can solve the problem of not taking into account the disinfection efficiency, the separation efficiency of the testa and seed kernel, the limitation of large-scale operation efficiency, and the prolongation of seed soaking Time and other issues, to achieve the effect of convenient large-scale embryo culture application, simple and easy operation, simple and convenient operation

Active Publication Date: 2015-12-02
ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The second is that the disinfection efficiency and the separation efficiency of the seed coat and the kernel cannot be well taken into account, which limits the efficiency of large-scale operation
In terms of operation, prolonging the soaking time of seeds in aqueous solution is beneficial to the separation of subsequent seed kernels, but it will reduce the vitality of seeds due to the artificial anaerobic respiration of seeds

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] 1) Preparation of medium

[0038] Prepare WPM medium for culturing peach embryos, and divide into corresponding plastic or glass test tubes to one-fifth to one-fourth of the height of the test tubes, cover with test tube stoppers, and cool at room temperature for later use after autoclaving;

[0039] 2) Fruit washing

[0040] After the ripe peach fruit is removed from the fruit stem, it is cleaned with tap water with an appropriate amount of washing liquid;

[0041] 3) Alcohol soaking

[0042] Peach fruit is soaked in 75% alcohol for 10-15 minutes in a clean room;

[0043] 4) Removal of seeds

[0044] Throw away the fruit with a core cutter, cut the seed core, and take out the seeds;

[0045] 5) Seed disinfection

[0046] ① Gradient H 2 o 2 Low concentration H in solution immersion 2 o 2 Solution immersion: seeds immersed in 0.3% H 2 o 2In the tissue culture bottle of the solution, until the experimental seeds are taken, the time should be less than 4h;

[0...

Embodiment 2

[0058] 1) Preparation of medium

[0059] Prepare WPM medium for culturing peach embryos, and divide into corresponding plastic or glass test tubes to one-fifth to one-fourth of the height of the test tubes, cover with test tube stoppers, and cool at room temperature for later use after autoclaving;

[0060] 2) Fruit washing

[0061] After the ripe peach fruit is removed from the fruit stem, it is cleaned with tap water with an appropriate amount of washing liquid;

[0062] 3) Alcohol soaking

[0063] Peach fruit is soaked in 75% alcohol for 10-15 minutes in a clean room;

[0064] 4) Removal of seeds

[0065] Throw away the fruit with a core cutter, cut the seed core, and take out the seeds;

[0066] 5) Seed disinfection

[0067] ① Gradient H 2 o 2 Low concentration H in solution immersion 2 o 2 Solution immersion: seeds immersed in 0.5% H 2 o 2 In the tissue culture bottle of the solution, until the experimental seeds are taken, the time should be less than 4h;

[...

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Abstract

The invention provides a technology for obtaining peach embryo aseptic seedlings on a large scale, and belongs to the technical field of plant biology. The technology includes the steps of culture medium preparation, peach fruit washing, surface steeping and sterilizing, seed separation, gradient H2O2 solution soaking, sterile water flushing, kernel separation and inoculation, low-temperature processing, room temperature seedling emergence, and the like. The gradient H2O2 solution soaking of seeds serves as the innovative technology characteristic. According to the technology, peroxidase in seed coats and endosperm reacts with H2O2 to release reactive oxygen so that microorganisms possibly carried by the seeds can be killed, and meanwhile the gradient H2O2 solution soaking is beneficial to separation of seed coats and kernels. The technology is easy to operate, high in safety, beneficial for conducting large-scale seed sterilization and seed coat and kernel separation of peach embryo culture on a large scale, the contamination rate is decreased, the inoculation efficiency is improved, and a new method is provided for obtaining the peach embryo aseptic seedlings on a large scale.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and specifically relates to a technology for obtaining peach embryos on a large scale and cultivating sterile seedlings. Background technique [0002] Peach is a drupe deciduous fruit tree, and its fruit is one of popular fruits. The fruit development time of peach early-maturing varieties is short, and the seed embryo is not yet mature when the fruit is ripe. Conventional stratification of early-maturing peach seeds has a very low emergence rate after sowing. Embryo culture technology can effectively improve the germination rate and seedling rate of early-maturing varieties of seeds, and is one of the necessary techniques for breeding early-maturing peach varieties. Peach embryo culture technology usually includes technical links such as medium preparation, fruit cleaning, seed removal, seed disinfection, sterile water washing, seed kernel separation and inoculation, low temperatu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 王新卫陈昌文王力荣朱更瑞曹珂方伟超王小丽
Owner ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI
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