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This invention belongs to the field of traditional Chinese medicine fermentation technology, specifically relating to a kidney-tonifying and aphrodisiac fermentation composition and its preparation method. The composition, by weight, comprises 30-60 parts of Cistanche deserticola, 30-60 parts of black truffle, 10-30 parts of black beans, 5-20 parts of Eucommia ulmoides male flowers, 15-30 parts of Codonopsis pilosula, 5-15 parts of enzyme preparation, 0.6-1.4 parts of fermentation starter culture, and 800-900 parts of water. The composition, with Cistanche deserticola and black truffle as the main components, supplemented by black beans, Eucommia ulmoides male flowers, and Codonopsis pilosula, works synergistically to achieve a good kidney-tonifying and aphrodisiac effect after enzymatic fermentation.