Method for discriminating cold resistance of peony tissue

A measurement method, peony technology, applied in the field of plant biology, can solve the problems affecting the accuracy of experimental results, poor repeatability, large data errors, etc., and achieve the effect of convenient low-temperature freezing damage protection, high feasibility, and simple method

Inactive Publication Date: 2013-06-26
HENAN UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods are complicated to operate, the analyzed data has large errors, and poor repeatability, which affects the accuracy of experimental results.

Method used

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  • Method for discriminating cold resistance of peony tissue
  • Method for discriminating cold resistance of peony tissue
  • Method for discriminating cold resistance of peony tissue

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Determination of Sepal Temperature Freezing Curve of Peony Variety Luhehong

[0027] A method for measuring the cold resistance of peony tissue, comprising the following steps:

[0028] A. Material collection: Select the Luhe red peony plants that are growing well in the germination stage, germination stage, leaf display stage, leaf opening stage, leaf extension stage, wind bell stage and dew color stage, and store them at room temperature to prevent dehydration of the peony plants.

[0029] B. Put the peony plants in step A into a polyethylene foam box. The length, width, height and wall thickness of the polyethylene foam box are 20cm, 20cm, 25cm, and 20mm respectively. Absorbent cotton is placed around the peony plants to ensure that the peony plants The internal temperature decreases slowly, causing the tissue to gradually release heat and freeze;

[0030] C. Connect the thermocouple thermometer to the computer, insert one probe of the thermocouple thermo...

Embodiment 2

[0034] Embodiment 2: choose Luoyang red and Fengdanbai to test respectively

[0035] The test materials 'Luoyang Red' and 'Fengdanbai' were collected from the Peony Garden of Henan University of Science and Technology from February to April 2012. Leaf stage, campanula stage, dew color stage. The freezing point and supercooling point temperature of different parts (old stems, scales, young leaves, bracts, sepals, petals) during the budding process were measured, and the difference in supercooling ability (ΔE) of peony flower organs at different development stages was calculated. (℃).

[0036] The result is as Figure 7 As shown, the supercooling ability of the same part of Luoyanghong and Fengdanbai's flower organs showed a weakening trend with the growth and development. The supercooling ability of the scales in the late germination stage is the weakest, Luoyang red (1.04±0.57℃), Fengdanbai (2.57±1.80℃). At this time, the content of various substances in the scales has cha...

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Abstract

The invention belongs to the technical field of plant biology and specifically relates to a method for discriminating the cold resistance of a peony tissue. The method provided by the invention is capable of directly reflecting the adaptability and the buffering ability of the peony tissue to the low-temperature environment and the obvious degree of supercooling by measuring the freezing point temperature and the supercooling temperature of the peony plant tissue part and defining the difference between the freezing point temperature and the supercooling temperature as peony tissue supercooling ability (delta E). The greater the value of delta E is, the more obvious the supercooling phenomenon of the tissue is, the larger the temperature zone sensitive to low-temperature freezing injury is and the higher the adaptability of responding to a sudden low temperature is. The method provided by the invention is simple and convenient, and high in feasibility; and the method is capable of obtaining the supercooling point temperature and the freezing point temperature of the plant tissue from a temperature freezing curve, thereby providing convenient and accurate scientific basis for low-temperature safe storage and low-temperature freeze injury protection of peony.

Description

technical field [0001] The invention belongs to the field of plant biology, and in particular relates to a method for measuring the cold resistance of peony tissues. Background technique [0002] Freezing injury (cold injury) is a kind of agricultural meteorological disaster, that is, the plant freezes in the tissue at a low temperature below 0 °C, causing damage to the plant, causing the cells in the plant tissue to freeze and losing all physiological activities, and eventually leading to the death of the plant. or partial death. Freezing damage to overwintering plant tissues, freezing damage to fruit trees, and freezing damage to commercial forest trees often occur, which are important limiting factors affecting plant growth. Plant tissue cold resistance is the ability of plants to resist or tolerate subzero temperatures, and it is the ability of plants to adapt to adverse environments formed during the long-term evolution process. The degree of plant damage depends on t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N25/00
Inventor 史国安王玮郭香凤孟海燕
Owner HENAN UNIV OF SCI & TECH
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