A Method for Indoor Identification of Cold Resistance of Wheat

Abstract

The invention provides a method for testing the cold resistance of wheat indoors, which comprises the following steps: seeding, vernalization, subzero low-temperature treatment and light treatment, and wheat seedling survival survey after low-temperature treatment. After low-temperature treatment, the higher the survival rate of wheat seedlings is, the better the cold resistance of the wheat seedlings is; according to the survival degrees of ordinary winter wheat seedlings treated by different low temperatures, the cold resistances of the ordinary winter wheat are divided into five classes, i.e., worse, bad, good, better and best. The method has the advantages as follows: the temperature range of controllable low-temperature treatment is wide (the lowest temperature can be as low as minus35 DEG C), the method is easy to operate, the accuracy of cold resistance testing is high, and moreover, the cold resistance degree of wheat can be determined. The method can be applied in the testingof cold resistances of wheat germplasm resources and sibling species thereof, the screening of cold resistances of wheat crossing parents, the testing of cold resistances of bred wheat offspring andnew varieties (lines), and the research of cold resistances of wheat varieties (lines).

Description

technical field [0001] The invention relates to the field of wheat biology research, in particular to a method for indoor identification of cold resistance of wheat. Background technique [0002] Wheat is one of the main food crops in the world, and 35% to 40% of the world's population depends on wheat for food. In China, the planting area of ​​wheat is second only to corn and rice. my country is mainly dominated by winter wheat, and its planting area and output both account for more than 82% of wheat. Wheat freezing injury in winter, early spring freezing injury (late spring cold) and low temperature freezing injury have become the main natural disasters threatening the safety of wheat production in my country. Freezing injury in winter is caused by the sudden drop in temperature from the beginning of winter to the overwintering period of wheat, and its occurrence is related to various factors such as variety, climate, soil foundation and cultivation methods. The agricul...

AI Technical Summary

Problems solved by technology

For example, the conventional cold resistance identification method is greatly affected by the external environment temperature, and warm winter weather often occurs in recent years, which not only seriously affects the accuracy of cold resistance identification, but also cannot accurately identify its cold resistance degree; the determination of physiological and biochemical indicators method, the operation steps are complicated, and the chemical reagents used are likely to cause environmental pollution, and a slight low temperature stress of wheat varieties (lines) can cause relatively large physiological and biochemical changes, while strong low temperature stress can easily cause the death of wheat plants, which cannot be measured according to the relevant indicators. Determine its cold tolerance

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