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Apocarya pollen in-vitro germination liquid culture medium and application thereof in measuring pollen activity

A technology of liquid culture medium and hickory shell is applied in the directions of measuring devices, plant cells, instruments, etc., which can solve the problems of inability to accurately reflect the pollen viability, difficulty in germination of hickory shell pollen, etc., and achieve stable and reliable measurement results. Effect

Inactive Publication Date: 2013-05-08
NANJING FORESTRY UNIV
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AI Technical Summary

Problems solved by technology

[0007] Pecan pollen germination is difficult, and the existing technology has not been able to break through
In Juglandaceae plants, only the pollen germination of walnut (Juglans regia L.) pollen has been studied, but the highest germination rate is only 2.22%, which cannot accurately reflect the pollen vigor

Method used

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  • Apocarya pollen in-vitro germination liquid culture medium and application thereof in measuring pollen activity
  • Apocarya pollen in-vitro germination liquid culture medium and application thereof in measuring pollen activity
  • Apocarya pollen in-vitro germination liquid culture medium and application thereof in measuring pollen activity

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Embodiment 1

[0030] 1. The collection of pecan pollen

[0031] Select healthy and healthy hickory pecan plants with normal development and no pests and diseases, pick the male flower inflorescences in time when the anthers turn yellow, put them in a sulfuric acid paper bag and quickly bring them back to the laboratory, spread them on the sulfuric acid paper, place them in a dry place away from the wind, Daily anthers can be loose powder. Sieve the pollen with a 120-mesh pharmacopoeia sieve, put it into a vacuum waterproof sealed bag, and store it in a -20°C refrigerator for later use.

[0032] 2. Screening of pollen rehydration time

[0033] with K 2 SO 4 or CuSO 4 ·5H 2 O saturated salt solution to adjust RH (Relative Humidity, relative humidity), let the pollen rehydrate in a closed container at 25°C and RH=97%, and place it for 0, 1, 2, 3, 4, 8, 12, and 24 hours respectively , with BK medium (containing 10%wt sucrose, 0.01%wtH 3 BO 3 , 0.03%wtCa (NO 3 ) 2 4H 2 O, 0.02%wtMgSO ...

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Abstract

The invention discloses apocarya pollen in-vitro germination liquid culture medium and application thereof in measuring pollen activity. The culture medium uses distilled water as solvent and contains sucrose, H3BO3 and Ca(NO3)2.4H2O. The cultivation method comprises hydration treatment, cultivation temperature and time adjustment. According to the apocarya pollen in-vitro germination liquid culture medium disclosed by the invention, the technical problems that the apocarya pollen germination rate is low and the germination tube growth is slow are solved, so that the pollen germination can be more than 70%. According to the culture method and the cultivation method disclosed by the invention, the pollen germination and growth of the apocarya are improved, the operation is simple, the cost is low, the period is short and the germination rate is high, so that the apocarya pollen in-vitro germination liquid culture medium has an extensive application prospect in the juglandaceae plant biology.

Description

technical field [0001] The invention belongs to the technical field of measuring plant pollen vigor, and in particular relates to a liquid culture medium for in vitro germination of pecan carya pollen and a method for measuring the vigor of pecan carya pollen using the culture medium. Background technique [0002] Carya illinoinensis is a plant of the genus Carya nutt of the family Julandaceae, also known as American hickory and long hickory. Thin-shell hickory is one of the world-renowned dried fruit tree species. Its nuts are large, thin-shelled, with high kernel yield, easy kernel extraction and high yield. Thin-shell hickory is also an important woody oil plant. Its oil content is as high as 70% and its unsaturated fatty acid content is as high as 97%. It is a high-quality cooking oil. Thin-shell hickory is also an excellent material and garden greening tree species. Its wood texture is fine and tough, and it is an ideal material for construction, military industry, int...

Claims

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Application Information

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IPC IPC(8): C12N5/04G01N33/00
Inventor 张瑞彭方仁梁有旺
Owner NANJING FORESTRY UNIV
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