Method for excavating natural hybridization germplasm and breeding new varieties of kiwi fruits

Abstract

The invention discloses a method for excavating natural hybridization germplasm and breeding new varieties of kiwi fruits and relates to the field of plant genetic breeding and biotechnology. The method comprises the steps of (1) surveying distribution conditions of mixed populations of species in a naturally overlapped distribution region of actinidia plants and collecting samples; (2) analyzing populations for assisting in positioning in a hybridization band through molecular markers; (3) collecting germplasm of the hybridization band and performing artificial domestication on the germplasm in a breeding garden; (4) commenting on and performing large-scale regional trial on superior plants or varieties obtained through artificial domestication; (5) registering and applying for new varieties. Compared with traditional artificial hybridization breeding, by means of the method, the breeding cycle is shortened greatly, the excavation geographic area of the target hybridization germplasm is narrowed down, blindness and randomness in utilization of the traditional natural germplasm of the kiwi fruits can be overcome, singular germplasm unexpected by traditional artificial breeding can be screened and domesticated, and problems caused by biosecurity are avoided.

Description

technical field [0001] The invention relates to the fields of plant genetics and breeding and biotechnology, in particular to a method for excavating kiwifruit natural hybrid germplasm and cultivating new varieties. It specifically involves the genetic evaluation of germplasm resources in natural overlapping distribution areas of different species of Actinidia, the use of molecular marker technology to assist in the positioning of natural hybrid zone populations within (inter) species, the discovery of excellent natural hybrid germplasm and the cultivation and domestication of artificial breeding gardens , and finally the breeding and release of new varieties. Background technique [0002] Kiwifruit (Actinidia Lindl.) is a kind of fruit that was artificially domesticated and cultivated in the early 20th century, and has a history of only more than 100 years. Kiwi fruit is widely favored for its unique flavor, rich in vitamin C, dietary fiber, multi-mineral nutrition, and fu...

AI Technical Summary

Problems solved by technology

Although some kiwifruit cultivars currently released are directly or indirectly derived from natural germplasm resources, the early excavation work is very random and blind, and no systematic and complete method has been formed for subsequent kiwifruit genetic breeding. For the exploration and utilization of natural hybrid germplasm resources, there is still a lack of convenient, quick and effective methods

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