Method for excavating natural hybridization germplasm and breeding new varieties of kiwi fruits

A breeding method and technology of new varieties, applied in the fields of plant genetics and biology, can solve the problems of lack of convenience, lack of utilization of kiwifruit genetics and breeding, etc., and achieve the effects of overcoming blindness and randomness, shortening the breeding period, and reducing geographical areas

Inactive Publication Date: 2014-11-26
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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Problems solved by technology

Although some kiwifruit cultivars currently released are directly or indirectly derived from natural germplasm resources, the early excavation work is very random and blind, and no systematic and complete method has been formed for subsequent kiwifruit genetic breeding. For the exploration and utilization of natural hybrid germplasm resources, there is still a lack of convenient, quick and effective methods

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  • Method for excavating natural hybridization germplasm and breeding new varieties of kiwi fruits
  • Method for excavating natural hybridization germplasm and breeding new varieties of kiwi fruits
  • Method for excavating natural hybridization germplasm and breeding new varieties of kiwi fruits

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Embodiment Construction

[0028] Below in conjunction with accompanying drawing and embodiment describe in detail:

[0029] 1. Method

[0030] 1. If figure 1 Step ① Investigate the distribution of species-mixed populations in the natural overlapping distribution area of ​​Actinidia and collect samples, mainly based on the existing six geographical distribution regions of Actinidia in China, and determine the main ones based on the geographical distribution and resource investigation of different species. Species mixed populations (≥ 2 species or variants), the identification of species is based on the internationally accepted kiwifruit UPOV (Union for the Protection of New Varieties of Plants, http: / / www.upov.int / genie / en / details.jsp ?id=101) describes the indicator. More than 10 mixed populations were screened for subsequent molecular marker genetic analysis and identification of hybridization bands in each major geographical area investigated. The representative sample collection method for mixed ...

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Abstract

The invention discloses a method for excavating natural hybridization germplasm and breeding new varieties of kiwi fruits and relates to the field of plant genetic breeding and biotechnology. The method comprises the steps of (1) surveying distribution conditions of mixed populations of species in a naturally overlapped distribution region of actinidia plants and collecting samples; (2) analyzing populations for assisting in positioning in a hybridization band through molecular markers; (3) collecting germplasm of the hybridization band and performing artificial domestication on the germplasm in a breeding garden; (4) commenting on and performing large-scale regional trial on superior plants or varieties obtained through artificial domestication; (5) registering and applying for new varieties. Compared with traditional artificial hybridization breeding, by means of the method, the breeding cycle is shortened greatly, the excavation geographic area of the target hybridization germplasm is narrowed down, blindness and randomness in utilization of the traditional natural germplasm of the kiwi fruits can be overcome, singular germplasm unexpected by traditional artificial breeding can be screened and domesticated, and problems caused by biosecurity are avoided.

Description

technical field [0001] The invention relates to the fields of plant genetics and breeding and biotechnology, in particular to a method for excavating kiwifruit natural hybrid germplasm and cultivating new varieties. It specifically involves the genetic evaluation of germplasm resources in natural overlapping distribution areas of different species of Actinidia, the use of molecular marker technology to assist in the positioning of natural hybrid zone populations within (inter) species, the discovery of excellent natural hybrid germplasm and the cultivation and domestication of artificial breeding gardens , and finally the breeding and release of new varieties. Background technique [0002] Kiwifruit (Actinidia Lindl.) is a kind of fruit that was artificially domesticated and cultivated in the early 20th century, and has a history of only more than 100 years. Kiwi fruit is widely favored for its unique flavor, rich in vitamin C, dietary fiber, multi-mineral nutrition, and fu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G17/00A01H1/02A01H1/04
Inventor 黄宏文刘义飞钟彩虹李大卫
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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