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43results about How to "No biosecurity issues" patented technology
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Industrial production method of hydrolyzed wheat protein for feeding
InactiveCN101627793AConfiguration highAdvanced configurationAnimal feeding stuffAccessory food factorsHydrolysateCombined treatment
The invention discloses an industrial production method of hydrolyzed wheat protein for feeding, belonging to the technical field of feed additive production. In the method, through the method of synergy of multiple enzymes and combined treatment of multiple steps, the hydrolyzed wheat protein for feeding is prepared by taking wheat gluten meal and water with weight ratio of 1:10-1:2 and adding compound enzyme, ensuring the mass percent concentration of the compound enzyme in the solution is 0.2-1.4%, hydrolyzing the mixture for 1-13h under the conditions that the temperature is 30-70 DEG C and pH value is 5.0-10.0, then carrying out such processes as filtering, decoloring and drying, etc. Compared with the traditional methods, the method of the invention features mild hydrolysis conditions, sanitation and safety and maintains the original nutritive composition of amino acid free of loss; the hydrolysate is low in salt content, mellow in mouth feel and high in yield which is up to 98%. The tests that the hydrolyzed wheat protein is added in the feed for suckling pigs, chicks, calves, aquaculture and the like to be used show that the hydrolyzed wheat protein has the advantages and evident effects of improving daily feed intake, reducing diarrhea rate, enhancing immunity, slowing weanling stress, improving growth performance and the like.
Owner:郑州新威营养技术有限公司
Preparation for porcine defensin engineering yeast strain
InactiveCN101275118AIncrease production capacityReduce manufacturing costFungiMicroorganism based processesYeast chromosomeBiotechnology
The present invention provides a preparing method of a porcine defensins engineering yeast strain, firstly, the porcine defensins gene is modified by method of genetic site-directed mutagenesis, mutant of porcine defensins gene having high biological activity is screened, then the porcine defensins gene which codon is optimized is artificially synthesized by the conventional codon of yeast strain, the engineering yeast strain having high yield, strong biological activity for producing porcine defensins is obtained by multiple screens. The porcine defensins engineering yeast strain kills the yeast by heating after fermented, directly used as feed additive by concentrated, having no separating and extracting courses, reducing the production cost of the defensins, improving the economic effect of the production enterprise. The invention provides conditions for mass industrial production of defensins, resolving the displacement of the porcine feed antibiotics additive.
Owner:HEBEI AGRICULTURAL UNIV.
Method for producing antigen protein in use for hog cholera vaccine
A process for preparing the antigen protein used for hog cholera vaccine includes extracting hog cholera virus RNA, reverse transcription to obtain the immune gene E2 of hog cholera virus, using E2 as template for PCR while inserting them to expression carrier of Bichia yeast, introducing the recombinant expression carrier to Bichia yeast, screening the recombinant Bichia yeast, discriminating its expression product testing its immunoactivity, determining the chosen recombinant yeast, testing and analyzing its culture condition, choosing the optimal culture condition, culturing the recombinant yeast and preparing the antigen protein of said vaccine.
Owner:LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
Crucian herpesvirus disease JDORF25 vaccine as well as preparation method and application thereof
ActiveCN104593388ASimple purification processReduce manufacturing costFungiVirus peptidesAntigenPichia pastoris
The invention discloses a crucian herpesvirus disease JDORF25 vaccine as well as a preparation method and an application thereof. The crucian herpesvirus disease vaccine is recombinant protein truncated, optimized and translated by crucian herpesvirus type II immuno-related ORF25 gene, wherein the sequence is shown in SEQ ID NO.2; the gene segment is synthesized, transferred to a yeast expression vector and screened by highly copying and cloning to finally obtain a high-expression recombinant protein pichia pastoris Km71 / CyHV-2-25 Pichia pastoris Km71 / CyHV-2-25, CCTCC NO: M2014570. The protein fermented by the yeast has the characteristics of high activity and high yield. The passive immunity test defines that the antigen protein can effectively prevent crucian herpesvirus disease and can also be mixed with adjuvants for highly effectively preventing crucian herpesvirus disease.
Owner:YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
Crucian herpes virus disease compound vaccine preparation, preparation method and application
ActiveCN104524564AImprove securityNo genetic recombination issuesFungiVirus peptidesChannel catfish herpesvirusYeast
The invention discloses a crucian herpes virus disease compound vaccine preparation, a preparation method and application. An applicant provides three strains of recombinant saccharomycetes, including pichia pastoris Km71 / CyHV-2-25, CCTCC NO:M2014570, pichia pastoris Km71 / CyHV-2-25C, CCTCC NO:M2014571 and pichia pastoris Km71 / CyHV-2-25D, CCTCC NO:M2014572. Proteins produced by utilizing the saccharomycetes through fermentation have the characteristics of high activity and yield; and immunity protective experiments prove that the crucian herpes virus disease compound vaccine preparation prepared by mixing the three proteins is capable of effectively preventing crucian herpes virus diseases.
Owner:YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
Synthetic peptide vaccine for treating porcine reproductive and respiratory syndrome and preparation method thereof
InactiveCN101845083AImprove securityImprove efficiencyViral antigen ingredientsVirus peptidesMedicinePorcine reproductive and respiratory syndrome virus
The invention provides a synthetic peptide vaccine for treating porcine reproductive and respiratory syndrome and a preparation method thereof, in particular relates to polypeptide of the synthetic peptide vaccine for treating the porcine reproductive and respiratory syndrome and a vaccine which contains the polypeptide and a method for preparing the polypeptide and the vaccine. The amino acid sequence of the polypeptide is an amino acid sequence shown in SEQ ID No.1, SEQ ID No.2, SEQ ID No.3 or SEQ ID No.4. The vaccine prepared from the polypeptide can effectively cope with the antigenic variation of a porcine reproductive and respiratory syndrome virus, ensures biological safety, is easy for large-scale synthesis and has good application prospect.
Owner:CHINA ANIMAL HUSBANDRY IND
Construction method and expression method of coexpression vector of L7/L12, Omp31, Rs alpha and sodC Brucella immune proteins
InactiveCN104152480AAvoid interactionGuaranteed stable expressionAntibacterial agentsBacterial antigen ingredientsGene engineeringBiology
The invention discloses a construction method and an expression method of a coexpression vector of L7 / L12, Omp31, Rs alpha and sodC Brucella immune proteins. The disclosed coexpression vector includes a double expression vector pETDuet-1 and a double expression vector pRSFDuet-1, which are both expressed in the same host bacteria, wherein the two double expression vectors are respectively connected with encoding genes of two Brucella immune proteins. On this basis, the invention also discloses the construction method and the expression method of the coexpression vector. L7 / L12, Omp31, Rs alpha and sodC Brucella immune proteins all with good immunity functions can be expressed simultaneously by the coexpression vector disclosed by the invention and are effectively used for preparing subunit vaccine of the Brucella gene engineering and preventing and controlling various brucellosis.
Owner:LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
Giant salamander iridescent virus vaccine, preparation method and application
ActiveCN104404057AImprove securityNo genetic recombination issuesFungiViral antigen ingredientsAntigenYeast
The invention discloses giant salamander iridescent virus vaccine, a preparation method and an application. The giant salamander iridescent virus vaccine is recombinant protein translated after truncating and optimizing a major capsid protein gene of a giant salamander iridescent virus, and has a sequence as shown as SEQIDNO.2 (Sequence Identifier Number 2). A nucleotide sequence corresponding to the protein is optimized according to preference of a yeast codon to synthesize a gene segment which is transferred to a yeast expression vector, and pichiapastoris Km71 / GSIV-MCP (i) Pichiapastoris(1 / i)Km71 / GSIV-MCP (CCTCC (China Center for Type Culture Collection) NO: M2014573) of the high expression recombinant protein is finally obtained via high copy and clone screening. The protein produced by fermentating the yeast has the characteristics of high activity and large yield. An immunoprotection experiment determines that the antigen protein can effectively prevent a giant salamander iridescent virus disease.
Owner:YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
Industrial production method of rice protein hydrolysate for feed
The invention discloses an industrial production method of rice protein hydrolysate for feed, and belongs to the technical field of feed additive production. The industrial production method of the rice protein hydrolysate for the feed comprises the steps that (1) the method of synergism of various enzymes and multi-step joint processing is adopted, the solid / liquid weight ratio between rice protein powder and water is 1:2-10, and alkaline proteinase is added to carry out dissolution; (2) a complex enzyme preparation which is formed by the alkaline proteinase, neutral proteinase, papain and flavourzyme in a compound mode is added to carry out hydrolysis, and the working procedures such as enzyme deactivation, filtration, decolorization and spray drying are carried out to obtain a rice protein hydrolysate product for the feed. Compared with the traditional method, the hydrolysis conditions are moderate, hygiene and safety are achieved, the situation that original amino-acid composition nutrients are not damaged is ensured, the hydrolysate is low in salinity and mellow in taste, and the yield is high and can reach 98%. According to application tests of adding the rice protein hydrolysate to feed such as feed of suckling pigs, feed of chicks, feed of calves and feed of aquaculture, the rice protein hydrolysate for the feed has the advantages of increasing the daily food-intake amount, reducing the diarrhea rate, improving immunity, reducing weaning stress, and improving growth performance.
Owner:江苏康科食品工程技术有限公司
Method for excavating natural hybridization germplasm and breeding new varieties of kiwi fruits
InactiveCN104160908AShorten the breeding periodReduce geographic areaCultivating equipmentsPlant genotype modificationActinidiaGermplasm
The invention discloses a method for excavating natural hybridization germplasm and breeding new varieties of kiwi fruits and relates to the field of plant genetic breeding and biotechnology. The method comprises the steps of (1) surveying distribution conditions of mixed populations of species in a naturally overlapped distribution region of actinidia plants and collecting samples; (2) analyzing populations for assisting in positioning in a hybridization band through molecular markers; (3) collecting germplasm of the hybridization band and performing artificial domestication on the germplasm in a breeding garden; (4) commenting on and performing large-scale regional trial on superior plants or varieties obtained through artificial domestication; (5) registering and applying for new varieties. Compared with traditional artificial hybridization breeding, by means of the method, the breeding cycle is shortened greatly, the excavation geographic area of the target hybridization germplasm is narrowed down, blindness and randomness in utilization of the traditional natural germplasm of the kiwi fruits can be overcome, singular germplasm unexpected by traditional artificial breeding can be screened and domesticated, and problems caused by biosecurity are avoided.
Owner:SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
Choroidal neovascularization targeting nanoparticle coated with macrophage membrane and preparation method of choroidal neovascularization targeting nanoparticle
PendingCN113425701AEasy to prepareLow requirements for experimental equipmentOrganic active ingredientsSenses disorderProtein proteinPoly l lactic acid
The invention discloses a choroidal neovascularization targeting nanoparticle coated with a macrophage membrane and a preparation method of the choroidal neovascularization targeting nanoparticle. A polylactic acid-glycolic acid copolymer entraps rapamycin to prepare rapamycin-PLGA nanoparticles, the rapamycin-PLGA nanoparticles are used as an inner core, and a macrophage membrane is used as a capsid to coat the surface of the rapamycin-PLGA nanoparticles; wherein the mass ratio of the rapamycin to the polylactic acid-glycolic acid copolymer is 1: (9-20), and the mass ratio of the protein in the macrophage membrane to the polylactic acid-glycolic acid copolymer is 1: (1-1.5). The prepared nanoparticles are good in biocompatibility, the preparation method is simple, and the particle size distribution of the nanoparticles is uniform. The key lesion of age-related macular degeneration, namely choroidal neovascularization, is actively targeted by using biomolecules on the surface of a macrophage membrane, so that the active recruitment of a drug-containing carrier to the neovascularization is effectively improved, and the effect of protecting retinal pigment epithelium under an inflammatory condition is also achieved while the neovascularization is inhibited.
Owner:赵晨
Method for improving breed by introducing panax quinquefolius DNA into rice
InactiveCN103290022ASimple and fast operationLow priceGenetic engineeringFermentationBiotechnologyDNA Solutions
The invention discloses a method for improving a breed by introducing panax quinquefolius DNA into rice. The method comprises the steps of: firstly extracting panax quinquefolius total DNA to prepare panax quinquefolius total DNA solution; secondly planting parent rice, introducing the panax quinquefolius total DNA solution into the parent rice after the rice blossoms and continuing planting to harvest rice containing panax quinquefolius total DNA; thirdly planting seeds of the rice containing panax quinquefolius total DNA, observing the planted rice group in the growth period and selecting a variant with one or a plurality of character differences from the parent rice from the rice group to harvest the variant; and finally planting variant seeds and breeding the improved breed of rice by a systemic breeding method. The method has the advantages that the growth period of the improved breed of rice bred by the method is ahead of time; the number of ears of the single plant, the total seed number per ear, the number of filled seeds and the ripening rate are improved to different extents; and the improved breed has excellent characters.
Owner:廖富东
Polypeptide fragment composition and application in preparation of porcine reproductive and respiratory syndrome vaccine thereof
InactiveCN102321180AEffective response to antigenic variationNo biosecurity issuesAntiviralsAntibody medical ingredientsAgricultural scienceActive component
The invention discloses a polypeptide fragment composition and an application in preparation of a porcine reproductive and respiratory syndrome vaccine thereof. The polypeptide fragment composition provided by the invention is a polypeptide shown in sequence 1 of the sequence table, a polypeptide shown in sequence 2 of the sequence table, a polypeptide shown in sequence 3 of the sequence table, apolypeptide shown in sequence 4 of the sequence table, a polypeptide shown in sequence 5 of the sequence table, and a polypeptide shown in sequence 6 of the sequence table. The polypeptide fragment composition provided by the invention can be used as an active component of a porcine reproductive and respiratory syndrome vaccine, and has great application value for the prevention and treatment of porcine reproductive and respiratory syndrome. The vaccine provided by the invention can effectively cope with antigenic variation of porcine reproductive and respiratory syndrome virus, has no biological safety problem, is suitable for large-scale synthesis, and has good application prospects.
Owner:INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
Asia I-type aftosa synthetic peptide vaccine
InactiveCN101659696AFree from attackHigh level of immune responseAntiviralsPeptide preparation methodsChemical synthesisComputer aid
The invention provides Asia I-type aftosa synthetic peptide vaccine, and in particular relates to polypeptide or polypeptide polymer thereof used in the vaccine as well as the vaccine containing the polypeptide or the polypeptide polymer thereof and a preparation method thereof. The polypeptide has amino acid sequences shown in SEQ ID No.1, SEQ ID No.2 and SEQ ID NO.3. The Asia I-type aftosa synthetic peptide vaccine carries out chemical synthesis of potential antigen site peptide segments by carrying out sequencing of domestic aftosa epidemic strains to study the variation of the main antigen sites of aftosa and combining with a computer-aided method to carry out aftosa antigen site analytical prediction; and candidate polypeptide antigens are screened out by carrying out large numbers of animal experiments to obtain polypeptide antigens which have high immunoreaction level and can protect animals from the attack of aftosa epidemic strains. The Asia I-type aftosa synthetic peptide vaccine prepared by the polypeptide antigens can effectively cope with the antigen variation of aftosa virus without causing biosafety problems and has easy large-scale synthesis, thereby having a good application prospect.
Owner:CHINA ANIMAL HUSBANDRY IND
Bird flu synthetic peptide vaccine and preparation method thereof
ActiveCN101628932ANo biosecurity issuesEase of large-scale synthesisVirus peptidesAntiviralsBird fluPeptide vaccine
The invention provides a bird flu synthetic peptide vaccine and a preparation method thereof, in particular to polypeptide used for the bird flu synthetic peptide vaccine or a polymer thereof, as well as a vaccine containing the polypeptide or the polymer thereof and both preparation methods thereof, wherein an amino acid sequence of the polypeptide is an amino acid sequence showed as SEQ ID NO.1 or SEQ ID NO.2. The vaccine made from the polypeptide or the polymer thereof can effectively deal to antigenic variation of bird flu virus, has no biological safety problem, can be synthesized easily in large scale and has favorable application prospects.
Owner:CHINA ANIMAL HUSBANDRY IND
Specific promoter in leguminous plant legume related tissue and application thereof
ActiveCN104673792AControl expression positionAvoid disruption of gene expression levelsVector-based foreign material introductionAngiosperms/flowering plantsCancer researchGene
The invention relates to a specific promoter in a leguminous plant legume related tissue and application thereof. The promoter is particularly suitable for driving the specific expression of exogenous genes in the leguminous plant legume related tissue, such as legumes or seed leaves, and the expression level of the exogenous genes in other tissues of leguminous plants is extremely low or the exogenous genes are not expressed in the other tissues of the leguminous plants. The expression specificity and specificity of the promoter are very high. The invention further provides constructions, expression kits, carriers and the like with the promoter. For transgenic leguminous plants containing the promoter disclosed by the invention, agronomic traits of the leguminous plants can be specifically improved, so that the influence on the other tissues of the leguminous plants caused by the introduction of the exogenous genes is effectively avoided, the exogenous genes can be specifically expressed in the legume related tissue, and thus, various related products are obtained.
Owner:CAS CENT FOR EXCELLENCE IN MOLECULAR PLANT SCI
Asia I synthetic peptide vaccine of foot and mouth disease
ActiveCN101643500AFree from attackHigh level of immune responseVirus peptidesAntiviralsChemical synthesisAnimal testing
The invention provides an Asia I synthetic peptide vaccine of a foot and mouth disease, in particular to a polypeptide or a polypeptide polymer thereof for the Asia I synthetic peptide vaccine of thefoot and mouth disease, the vaccine containing the polypeptide or the polypeptide polymer thereof, and a preparation method of both, wherein, the amino acid sequence of the polypeptide is shown as SEQID NO:1. In the invention, variation situation of main antigen sites of the foot and mouth disease is researched by determining a sequence of an epidemic strain of the foot and mouth disease, and meanwhile analysis prediction of the antigen sites of the foot and mouth disease is performed followed by chemical synthesis on possible peptide segments of the antigen sites combined with a computer auxiliary method; and candidate polypeptide antigens are screened through a large quantity of animal experiments to finally obtain the polypeptide antigens with high immune reaction level which can protect well an animal from being attacked by the epidemic strain of the foot and mouth disease. The vaccine prepared from the polypeptide antigens has the advantages of no biological safety problem, easylarge-scale synthesis, good application prospect and good ability of effectively dealing with antigen variation of foot and mouth disease virus.
Owner:CHINA ANIMAL HUSBANDRY IND
Coexpression vector of Chlamydophila abortus and Chlamydophila psittaci protective antigen MOMP and MIP, construction and expression method thereof
ActiveCN104131020AGuarantee safe and effectiveAchieve quality controlChlamydiaceae ingredientsDepsipeptidesProtective antigenControl animal
The invention discloses a coexpression vector of Chlamydophila abortus and Chlamydophila psittaci protective antigen MOMP and MIP, a construction method and an expression method thereof. By means of a molecular cloning technique, the coding genes of Chlamydophila abortus's main outer membrane protein (MOMPa) and macrophage infection potentiator (MIPa) and Chlamydophila psittaci's main outer membrane protein (MOMPp) and macrophage infectivity potentiator (MIPp) are respectively connected to the dual expression vector pETDuet-1 and pRSFDuet-1 so as to construct the coexpression vector. The coexpression vector provided by the invention carries four protein genes of MOMP and MIP of Chlamydophila abortus and Chlamydophila psittaci, can realize expression in one host bacterium, and can be used for preparing polyvalent chlamydial genetic engineering subunit vaccines and preventing and controlling multi-serotype animal infectious diseases, is in favor of improving the technological level for controlling animal chlamydial diseases in China, thus ensuring the sound development of livestock raising industry, increasing the income of farmers and herdsmen, and guaranteeing public health and animal product safety.
Owner:LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
Escherichia coli strain reformed by gene engineering, and applications thereof
ActiveCN110352961AEnhanced inhibitory effectNo biosecurity issuesBiocideBacteriaEscherichia coliMetabolite
The invention discloses an Escherichia coli strain reformed by gene engineering, and applications thereof. According to the present invention, engineering bacteria for preventing and controlling smutis constructed by using the applications of methyl jasmonate in prevention and controlling of smut, and recipient bacteria over-express methyl jasmonate transferase; the metabolite of the engineeringbacteria is methyl jasmonate, such that the biosafety problem does not exist; jasmonate methyltransferase is derived from plants, and cannot cause biosafety problem after being expressed by Escherichia coli; the engineering bacteria are Escherichia coli, and has characteristics of simple culture conditions, low cost and simple implementation method; the method for improving the defense ability byincreasing the content of the hormone methyl jasmonate in the plant does not affect the growth of the plant; and with the application of the engineering bacteria in prevention and controlling of CornLump Smut, the significant prevention and control effect can be achieved, and the Escherichia coli strain reformed by gene engineering is worthy of being popularized and applied.
Owner:SOUTH CHINA AGRI UNIV
Bird flu synthetic peptide vaccine and preparation method thereof
ActiveCN101628932BNo biosecurity issuesEase of large-scale synthesisVirus peptidesAntiviralsBird fluAvian influenza virus
The invention provides a bird flu synthetic peptide vaccine and a preparation method thereof, in particular to polypeptide used for the bird flu synthetic peptide vaccine or a polymer thereof, as well as a vaccine containing the polypeptide or the polymer thereof and both preparation methods thereof, wherein an amino acid sequence of the polypeptide is an amino acid sequence showed as SEQ ID NO.1or SEQ ID NO.2. The vaccine made from the polypeptide or the polymer thereof can effectively deal to antigenic variation of bird flu virus, has no biological safety problem, can be synthesized easilyin large scale and has favorable application prospects.
Owner:CHINA ANIMAL HUSBANDRY IND
A kind of cultivation method of high-quality and high-yield salvia miltiorrhiza
ActiveCN105724238BIncrease productionImprove qualityCultivating equipmentsRoot crop cultivationSalvia miltiorrhizaHybrid seed
Owner:HENAN UNIV OF CHINESE MEDICINE
A kind of industrial production method of wheat hydrolyzed protein for feed
InactiveCN101627793BImprove digestion utilizationPromote absorptionAnimal feeding stuffAccessory food factorsHydrolysateCombined treatment
The invention discloses an industrial production method of hydrolyzed wheat protein for feeding, belonging to the technical field of feed additive production. In the method, through the method of synergy of multiple enzymes and combined treatment of multiple steps, the hydrolyzed wheat protein for feeding is prepared by taking wheat gluten meal and water with weight ratio of 1:10-1:2 and adding compound enzyme, ensuring the mass percent concentration of the compound enzyme in the solution is 0.2-1.4%, hydrolyzing the mixture for 1-13h under the conditions that the temperature is 30-70 DEG C and pH value is 5.0-10.0, then carrying out such processes as filtering, decoloring and drying, etc. Compared with the traditional methods, the method of the invention features mild hydrolysis conditions, sanitation and safety and maintains the original nutritive composition of amino acid free of loss; the hydrolysate is low in salt content, mellow in mouth feel and high in yield which is up to 98%. The tests that the hydrolyzed wheat protein is added in the feed for suckling pigs, chicks, calves, aquaculture and the like to be used show that the hydrolyzed wheat protein has the advantages and evident effects of improving daily feed intake, reducing diarrhea rate, enhancing immunity, slowing weanling stress, improving growth performance and the like.
Owner:郑州新威营养技术有限公司
New antigen display system based on insect viral capsid and construction method of new antigen display system
ActiveCN110144367AStrong immune responseBig spaceSsRNA viruses negative-senseSsRNA viruses positive-senseHeterologousInsertion site
The invention discloses a new antigen display system based on insect viral capsid and a construction method of the new antigen display system. The steps of the system construction method are as follows: (1) determining an insertion site of a heterologous protein; (2) successfully constructing a library of WhNV recombinant viroid particle VLP fused with enhanced green fluorescent protein EGFP; (3)constructing a library of Wuhan Noda Village recombinant virus particles fused with H5 antigen protein of human avian influenza virus; and (4) establishing a virus display system of Wuhan Noda Village. The system comprises: (1) WhNV capsid of Wuhan Noda Village virus, (2) a fusion protein as the heterologous protein inserted into the WhNV capsid protein, and (3) linkers, small amino acid sequencesrespectively added to the N-terminal and C-terminal of the fusion protein as linkers to connect with the WhNV capsid. According to the present invention, the construction method is simple and easy tooperate, and the WhNV heterologous antigen display system successfully established by the method lays a good foundation for the establishment of an efficient, stable and highly versatile platform forthe development of influenza VLP vaccine, and then for the establishment of platforms for the development of multiple vaccines and drugs.
Owner:WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
A kind of crucian carp herpes virus disease jdorf25 vaccine and its preparation method and application
ActiveCN104593388BImprove securityNo genetic recombination issuesFungiVirus peptidesPichia pastorisAdjuvant
The invention discloses a crucian herpesvirus disease JDORF25 vaccine as well as a preparation method and an application thereof. The crucian herpesvirus disease vaccine is recombinant protein truncated, optimized and translated by crucian herpesvirus type II immuno-related ORF25 gene, wherein the sequence is shown in SEQ ID NO.2; the gene segment is synthesized, transferred to a yeast expression vector and screened by highly copying and cloning to finally obtain a high-expression recombinant protein pichia pastoris Km71 / CyHV-2-25 Pichia pastoris Km71 / CyHV-2-25, CCTCC NO: M2014570. The protein fermented by the yeast has the characteristics of high activity and high yield. The passive immunity test defines that the antigen protein can effectively prevent crucian herpesvirus disease and can also be mixed with adjuvants for highly effectively preventing crucian herpesvirus disease.
Owner:YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
Co-expression vector of protective antigens momp and mip of Chlamydia abortus and Chlamydia psittaci and its construction and expression method
ActiveCN104131020BGuarantee safe and effectiveAchieve quality controlChlamydiaceae ingredientsDepsipeptidesAgricultural scienceChlamydophila
The invention discloses a coexpression vector of Chlamydophila abortus and Chlamydophila psittaci protective antigen MOMP and MIP, a construction method and an expression method thereof. By means of a molecular cloning technique, the coding genes of Chlamydophila abortus's main outer membrane protein (MOMPa) and macrophage infection potentiator (MIPa) and Chlamydophila psittaci's main outer membrane protein (MOMPp) and macrophage infectivity potentiator (MIPp) are respectively connected to the dual expression vector pETDuet-1 and pRSFDuet-1 so as to construct the coexpression vector. The coexpression vector provided by the invention carries four protein genes of MOMP and MIP of Chlamydophila abortus and Chlamydophila psittaci, can realize expression in one host bacterium, and can be used for preparing polyvalent chlamydial genetic engineering subunit vaccines and preventing and controlling multi-serotype animal infectious diseases, is in favor of improving the technological level for controlling animal chlamydial diseases in China, thus ensuring the sound development of livestock raising industry, increasing the income of farmers and herdsmen, and guaranteeing public health and animal product safety.
Owner:LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
A kind of bird flu synthetic peptide vaccine and preparation method thereof
ActiveCN109206492BSynthetic Effective CopingNo biosecurity issuesSsRNA viruses negative-senseViral antigen ingredientsChemical synthesisEngineering
Owner:CHINA ANIMAL HUSBANDRY IND
Legume root tissue-specific promoter and its application
ActiveCN104673793BControl expression positionAvoid disruption of gene expression levelsVector-based foreign material introductionAngiosperms/flowering plantsMyriophyllumPlant tissue
The invention relates to a specific promoter in root tissue of leguminous plants and its application. The promoter is particularly suitable for driving the specific expression of the exogenous gene in the root tissue of the legume plant, while the expression level in other tissues of the legume plant is extremely low or not expressed. The expression specificity and specificity of the promoter are very high. The present invention also provides constructs, expression cassettes, vectors and the like having the promoter. The transgenic legume plant containing the promoter of the present invention can specifically improve the agronomic traits of the legume plant, effectively avoid the impact of foreign gene introduction on other tissues of the legume plant, and can also specifically express the foreign gene in the root tissue, Thereby obtaining various related products.
Owner:CAS CENT FOR EXCELLENCE IN MOLECULAR PLANT SCI
Specific promoter in leguminous plant root tissue and application thereof
ActiveCN104673793AControl expression positionAvoid disruption of gene expression levelsVector-based foreign material introductionAngiosperms/flowering plantsPlant rootsFabaceae
The invention relates to a specific promoter in a leguminous plant root system tissue and application thereof. The promoter is particularly suitable for driving the specific expression of exogenous genes in the leguminous plant root tissue, and the expression level of the exogenous genes in other tissues of leguminous plants is extremely low or the exogenous genes are not expressed in the other tissues of the leguminous plants. The expression specificity and specificity of the promoter are very high. The invention further provides constructions, expression kits, carriers and the like with the promoter. For transgenic leguminous plants containing the promoter disclosed by the invention, agronomic traits of the leguminous plants can be specifically improved, so that the influence on the other tissues of the leguminous plants caused by the introduction of the exogenous genes is effectively avoided, the exogenous genes can be specifically expressed in the root tissue, and thus, various related products are obtained.
Owner:CAS CENT FOR EXCELLENCE IN MOLECULAR PLANT SCI
Preparation method of Brucella vaccine and antigenic protein for vaccine
InactiveCN101607081BNo genetic recombination issuesNo possible gene recombination problems will occurAntibacterial agentsBacterial antigen ingredientsBacteroidesDisease
The invention discloses an anti-brucellosis vaccine used by domestic animals and a preparation method of the antigenic protein used in the vaccine. The antigenic protein used in the Brucella vaccine of the present invention is the Brucella bcsp31 protein, especially the Brucella bcsp31 recombinant protein. The preparation method of the recombinant antigenic protein is to use the bacterial total DNA extracted from Brucella as a template, design a pair of primers according to the complete sequence of nucleotides of the Brucella bcsp31 gene, and carry out polymerase chain reaction, clone and obtain the bcsp31 protein gene, Then transfer to the yeast expression vector to obtain the recombinant expression vector, and then introduce it into the yeast expression bacteria to obtain the recombinant Pichia expression bacteria, proliferate and induce the expression of the recombinant Pichia expression bacteria, and obtain the antigen protein used by separation and purification.
Owner:LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
Asia I synthetic peptide vaccine of foot and mouth disease
ActiveCN101643500BFree from attackHigh level of immune responseVirus peptidesAntiviralsChemical synthesisFoot mouth disease virus
The invention provides an Asia I synthetic peptide vaccine of a foot and mouth disease, in particular to a polypeptide or a polypeptide polymer thereof for the Asia I synthetic peptide vaccine of the foot and mouth disease, the vaccine containing the polypeptide or the polypeptide polymer thereof, and a preparation method of both, wherein, the amino acid sequence of the polypeptide is shown as SEQ ID NO:1. In the invention, variation situation of main antigen sites of the foot and mouth disease is researched by determining a sequence of an epidemic strain of the foot and mouth disease, and meanwhile analysis prediction of the antigen sites of the foot and mouth disease is performed followed by chemical synthesis on possible peptide segments of the antigen sites combined with a computer auxiliary method; and candidate polypeptide antigens are screened through a large quantity of animal experiments to finally obtain the polypeptide antigens with high immune reaction level which can protect well an animal from being attacked by the epidemic strain of the foot and mouth disease. The vaccine prepared from the polypeptide antigens has the advantages of no biological safety problem, easylarge-scale synthesis, good application prospect and good ability of effectively dealing with antigen variation of foot and mouth disease virus.
Owner:CHINA ANIMAL HUSBANDRY IND
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