43results about How to "No biosecurity issues" patented technology

The invention relates to a good-quality and high-yield salvia miltiorrhiza cultivation method. The good-quality and high-yield salvia miltiorrhiza cultivation method can effectively solve the existing problems of low yield and poor quality of salvia miltiorrhiza and comprises the steps that hybrid seeds are cultivated, artificial pollination is performed, then paper bags are put on, the paper bag shredding or breaking indicates that accidental pollination occurs possibly, the pollination is performed again, the bags are timely taken on 3-5 days after pollination, growth management and prevention and control of plant diseases and insect pests are performed, digging and collection are performed when the salvia miltiorrhiza grows for 600-630 days, and the salvia miltiorrhiza plants with 30-50 divided roots respectively are selected for the hybrid seeds. The good-quality and high-yield salvia miltiorrhiza cultivation method can improve the yield and quality of cultivated salvia miltiorrhiza, can overcome the shortcomings of blindness and randomness during natural germplasm utilization of traditional salvia miltiorrhiza, can develop extraordinary germplasm which cannot be expected and obtained through traditional artificial breeding, does not produce the problem of biological safety, reduces the labor intensity for seed production, saves labors, reduces the production cost, makes separation of parents and the hybrid seeds accurate and makes the purity of produced seed high. The good-quality and high-yield salvia miltiorrhiza cultivation method can be used for larger-area hybrid seed production and is easy to popularize.

The invention discloses an industrial production method of hydrolyzed wheat protein for feeding, belonging to the technical field of feed additive production. In the method, through the method of synergy of multiple enzymes and combined treatment of multiple steps, the hydrolyzed wheat protein for feeding is prepared by taking wheat gluten meal and water with weight ratio of 1:10-1:2 and adding compound enzyme, ensuring the mass percent concentration of the compound enzyme in the solution is 0.2-1.4%, hydrolyzing the mixture for 1-13h under the conditions that the temperature is 30-70 DEG C and pH value is 5.0-10.0, then carrying out such processes as filtering, decoloring and drying, etc. Compared with the traditional methods, the method of the invention features mild hydrolysis conditions, sanitation and safety and maintains the original nutritive composition of amino acid free of loss; the hydrolysate is low in salt content, mellow in mouth feel and high in yield which is up to 98%. The tests that the hydrolyzed wheat protein is added in the feed for suckling pigs, chicks, calves, aquaculture and the like to be used show that the hydrolyzed wheat protein has the advantages and evident effects of improving daily feed intake, reducing diarrhea rate, enhancing immunity, slowing weanling stress, improving growth performance and the like.

The invention discloses a coexpression vector of Chlamydophila abortus and Chlamydophila psittaci protective antigen MOMP and MIP, a construction method and an expression method thereof. By means of a molecular cloning technique, the coding genes of Chlamydophila abortus's main outer membrane protein (MOMPa) and macrophage infection potentiator (MIPa) and Chlamydophila psittaci's main outer membrane protein (MOMPp) and macrophage infectivity potentiator (MIPp) are respectively connected to the dual expression vector pETDuet-1 and pRSFDuet-1 so as to construct the coexpression vector. The coexpression vector provided by the invention carries four protein genes of MOMP and MIP of Chlamydophila abortus and Chlamydophila psittaci, can realize expression in one host bacterium, and can be used for preparing polyvalent chlamydial genetic engineering subunit vaccines and preventing and controlling multi-serotype animal infectious diseases, is in favor of improving the technological level for controlling animal chlamydial diseases in China, thus ensuring the sound development of livestock raising industry, increasing the income of farmers and herdsmen, and guaranteeing public health and animal product safety.

The invention provides a polypeptide antigen for preparing a bird flu synthetic peptide vaccine, a vaccine containing the polypeptide antigen and a preparation method thereof. Polypeptide antigens include the polypeptides shown in Sequence 1. The avian influenza synthetic peptide vaccine provided by the invention has good immune efficacy, and the antigen of the vaccine is a chemically synthesized polypeptide, does not contain avian influenza virus nucleic acid particles, and has high safety. Therefore, the vaccine of the invention can effectively deal with the antigenic variation of the avian influenza virus, has no biological safety, is easy to synthesize on a large scale, and has good application prospects.

The invention relates to a specific promoter in root tissue of leguminous plants and its application. The promoter is particularly suitable for driving the specific expression of the exogenous gene in the root tissue of the legume plant, while the expression level in other tissues of the legume plant is extremely low or not expressed. The expression specificity and specificity of the promoter are very high. The present invention also provides constructs, expression cassettes, vectors and the like having the promoter. The transgenic legume plant containing the promoter of the present invention can specifically improve the agronomic traits of the legume plant, effectively avoid the impact of foreign gene introduction on other tissues of the legume plant, and can also specifically express the foreign gene in the root tissue, Thereby obtaining various related products.

The invention discloses an anti-brucellosis vaccine used by domestic animals and a preparation method of the antigenic protein used in the vaccine. The antigenic protein used in the Brucella vaccine of the present invention is the Brucella bcsp31 protein, especially the Brucella bcsp31 recombinant protein. The preparation method of the recombinant antigenic protein is to use the bacterial total DNA extracted from Brucella as a template, design a pair of primers according to the complete sequence of nucleotides of the Brucella bcsp31 gene, and carry out polymerase chain reaction, clone and obtain the bcsp31 protein gene, Then transfer to the yeast expression vector to obtain the recombinant expression vector, and then introduce it into the yeast expression bacteria to obtain the recombinant Pichia expression bacteria, proliferate and induce the expression of the recombinant Pichia expression bacteria, and obtain the antigen protein used by separation and purification.

The invention provides an Asia I synthetic peptide vaccine of a foot and mouth disease, in particular to a polypeptide or a polypeptide polymer thereof for the Asia I synthetic peptide vaccine of the foot and mouth disease, the vaccine containing the polypeptide or the polypeptide polymer thereof, and a preparation method of both, wherein, the amino acid sequence of the polypeptide is shown as SEQ ID NO:1. In the invention, variation situation of main antigen sites of the foot and mouth disease is researched by determining a sequence of an epidemic strain of the foot and mouth disease, and meanwhile analysis prediction of the antigen sites of the foot and mouth disease is performed followed by chemical synthesis on possible peptide segments of the antigen sites combined with a computer auxiliary method; and candidate polypeptide antigens are screened through a large quantity of animal experiments to finally obtain the polypeptide antigens with high immune reaction level which can protect well an animal from being attacked by the epidemic strain of the foot and mouth disease. The vaccine prepared from the polypeptide antigens has the advantages of no biological safety problem, easylarge-scale synthesis, good application prospect and good ability of effectively dealing with antigen variation of foot and mouth disease virus.