43results about How to "No biosecurity issues" patented technology

The invention discloses an industrial production method of hydrolyzed wheat protein for feeding, belonging to the technical field of feed additive production. In the method, through the method of synergy of multiple enzymes and combined treatment of multiple steps, the hydrolyzed wheat protein for feeding is prepared by taking wheat gluten meal and water with weight ratio of 1:10-1:2 and adding compound enzyme, ensuring the mass percent concentration of the compound enzyme in the solution is 0.2-1.4%, hydrolyzing the mixture for 1-13h under the conditions that the temperature is 30-70 DEG C and pH value is 5.0-10.0, then carrying out such processes as filtering, decoloring and drying, etc. Compared with the traditional methods, the method of the invention features mild hydrolysis conditions, sanitation and safety and maintains the original nutritive composition of amino acid free of loss; the hydrolysate is low in salt content, mellow in mouth feel and high in yield which is up to 98%. The tests that the hydrolyzed wheat protein is added in the feed for suckling pigs, chicks, calves, aquaculture and the like to be used show that the hydrolyzed wheat protein has the advantages and evident effects of improving daily feed intake, reducing diarrhea rate, enhancing immunity, slowing weanling stress, improving growth performance and the like.

The invention discloses a crucian herpesvirus disease JDORF25 vaccine as well as a preparation method and an application thereof. The crucian herpesvirus disease vaccine is recombinant protein truncated, optimized and translated by crucian herpesvirus type II immuno-related ORF25 gene, wherein the sequence is shown in SEQ ID NO.2; the gene segment is synthesized, transferred to a yeast expression vector and screened by highly copying and cloning to finally obtain a high-expression recombinant protein pichia pastoris Km71/CyHV-2-25 Pichia pastoris Km71/CyHV-2-25, CCTCC NO: M2014570. The protein fermented by the yeast has the characteristics of high activity and high yield. The passive immunity test defines that the antigen protein can effectively prevent crucian herpesvirus disease and can also be mixed with adjuvants for highly effectively preventing crucian herpesvirus disease.

The invention discloses a crucian herpes virus disease compound vaccine preparation, a preparation method and application. An applicant provides three strains of recombinant saccharomycetes, including pichia pastoris Km71/CyHV-2-25, CCTCC NO:M2014570, pichia pastoris Km71/CyHV-2-25C, CCTCC NO:M2014571 and pichia pastoris Km71/CyHV-2-25D, CCTCC NO:M2014572. Proteins produced by utilizing the saccharomycetes through fermentation have the characteristics of high activity and yield; and immunity protective experiments prove that the crucian herpes virus disease compound vaccine preparation prepared by mixing the three proteins is capable of effectively preventing crucian herpes virus diseases.

The invention provides a synthetic peptide vaccine for treating porcine reproductive and respiratory syndrome and a preparation method thereof, in particular relates to polypeptide of the synthetic peptide vaccine for treating the porcine reproductive and respiratory syndrome and a vaccine which contains the polypeptide and a method for preparing the polypeptide and the vaccine. The amino acid sequence of the polypeptide is an amino acid sequence shown in SEQ ID No.1, SEQ ID No.2, SEQ ID No.3 or SEQ ID No.4. The vaccine prepared from the polypeptide can effectively cope with the antigenic variation of a porcine reproductive and respiratory syndrome virus, ensures biological safety, is easy for large-scale synthesis and has good application prospect.

The invention discloses a construction method and an expression method of a coexpression vector of L7/L12, Omp31, Rs alpha and sodC Brucella immune proteins. The disclosed coexpression vector includes a double expression vector pETDuet-1 and a double expression vector pRSFDuet-1, which are both expressed in the same host bacteria, wherein the two double expression vectors are respectively connected with encoding genes of two Brucella immune proteins. On this basis, the invention also discloses the construction method and the expression method of the coexpression vector. L7/L12, Omp31, Rs alpha and sodC Brucella immune proteins all with good immunity functions can be expressed simultaneously by the coexpression vector disclosed by the invention and are effectively used for preparing subunit vaccine of the Brucella gene engineering and preventing and controlling various brucellosis.

The invention discloses giant salamander iridescent virus vaccine, a preparation method and an application. The giant salamander iridescent virus vaccine is recombinant protein translated after truncating and optimizing a major capsid protein gene of a giant salamander iridescent virus, and has a sequence as shown as SEQIDNO.2 (Sequence Identifier Number 2). A nucleotide sequence corresponding to the protein is optimized according to preference of a yeast codon to synthesize a gene segment which is transferred to a yeast expression vector, and pichiapastoris Km71/GSIV-MCP (i) Pichiapastoris(1/i)Km71/GSIV-MCP (CCTCC (China Center for Type Culture Collection) NO: M2014573) of the high expression recombinant protein is finally obtained via high copy and clone screening. The protein produced by fermentating the yeast has the characteristics of high activity and large yield. An immunoprotection experiment determines that the antigen protein can effectively prevent a giant salamander iridescent virus disease.

The invention discloses an industrial production method of rice protein hydrolysate for feed, and belongs to the technical field of feed additive production. The industrial production method of the rice protein hydrolysate for the feed comprises the steps that (1) the method of synergism of various enzymes and multi-step joint processing is adopted, the solid/liquid weight ratio between rice protein powder and water is 1:2-10, and alkaline proteinase is added to carry out dissolution; (2) a complex enzyme preparation which is formed by the alkaline proteinase, neutral proteinase, papain and flavourzyme in a compound mode is added to carry out hydrolysis, and the working procedures such as enzyme deactivation, filtration, decolorization and spray drying are carried out to obtain a rice protein hydrolysate product for the feed. Compared with the traditional method, the hydrolysis conditions are moderate, hygiene and safety are achieved, the situation that original amino-acid composition nutrients are not damaged is ensured, the hydrolysate is low in salinity and mellow in taste, and the yield is high and can reach 98%. According to application tests of adding the rice protein hydrolysate to feed such as feed of suckling pigs, feed of chicks, feed of calves and feed of aquaculture, the rice protein hydrolysate for the feed has the advantages of increasing the daily food-intake amount, reducing the diarrhea rate, improving immunity, reducing weaning stress, and improving growth performance.

The invention discloses a choroidal neovascularization targeting nanoparticle coated with a macrophage membrane and a preparation method of the choroidal neovascularization targeting nanoparticle. A polylactic acid-glycolic acid copolymer entraps rapamycin to prepare rapamycin-PLGA nanoparticles, the rapamycin-PLGA nanoparticles are used as an inner core, and a macrophage membrane is used as a capsid to coat the surface of the rapamycin-PLGA nanoparticles; wherein the mass ratio of the rapamycin to the polylactic acid-glycolic acid copolymer is 1: (9-20), and the mass ratio of the protein in the macrophage membrane to the polylactic acid-glycolic acid copolymer is 1: (1-1.5). The prepared nanoparticles are good in biocompatibility, the preparation method is simple, and the particle size distribution of the nanoparticles is uniform. The key lesion of age-related macular degeneration, namely choroidal neovascularization, is actively targeted by using biomolecules on the surface of a macrophage membrane, so that the active recruitment of a drug-containing carrier to the neovascularization is effectively improved, and the effect of protecting retinal pigment epithelium under an inflammatory condition is also achieved while the neovascularization is inhibited.

The invention discloses a method for improving a breed by introducing panax quinquefolius DNA into rice. The method comprises the steps of: firstly extracting panax quinquefolius total DNA to prepare panax quinquefolius total DNA solution; secondly planting parent rice, introducing the panax quinquefolius total DNA solution into the parent rice after the rice blossoms and continuing planting to harvest rice containing panax quinquefolius total DNA; thirdly planting seeds of the rice containing panax quinquefolius total DNA, observing the planted rice group in the growth period and selecting a variant with one or a plurality of character differences from the parent rice from the rice group to harvest the variant; and finally planting variant seeds and breeding the improved breed of rice by a systemic breeding method. The method has the advantages that the growth period of the improved breed of rice bred by the method is ahead of time; the number of ears of the single plant, the total seed number per ear, the number of filled seeds and the ripening rate are improved to different extents; and the improved breed has excellent characters.

The invention discloses a polypeptide fragment composition and an application in preparation of a porcine reproductive and respiratory syndrome vaccine thereof. The polypeptide fragment composition provided by the invention is a polypeptide shown in sequence 1 of the sequence table, a polypeptide shown in sequence 2 of the sequence table, a polypeptide shown in sequence 3 of the sequence table, apolypeptide shown in sequence 4 of the sequence table, a polypeptide shown in sequence 5 of the sequence table, and a polypeptide shown in sequence 6 of the sequence table. The polypeptide fragment composition provided by the invention can be used as an active component of a porcine reproductive and respiratory syndrome vaccine, and has great application value for the prevention and treatment of porcine reproductive and respiratory syndrome. The vaccine provided by the invention can effectively cope with antigenic variation of porcine reproductive and respiratory syndrome virus, has no biological safety problem, is suitable for large-scale synthesis, and has good application prospects.

The invention provides Asia I-type aftosa synthetic peptide vaccine, and in particular relates to polypeptide or polypeptide polymer thereof used in the vaccine as well as the vaccine containing the polypeptide or the polypeptide polymer thereof and a preparation method thereof. The polypeptide has amino acid sequences shown in SEQ ID No.1, SEQ ID No.2 and SEQ ID NO.3. The Asia I-type aftosa synthetic peptide vaccine carries out chemical synthesis of potential antigen site peptide segments by carrying out sequencing of domestic aftosa epidemic strains to study the variation of the main antigen sites of aftosa and combining with a computer-aided method to carry out aftosa antigen site analytical prediction; and candidate polypeptide antigens are screened out by carrying out large numbers of animal experiments to obtain polypeptide antigens which have high immunoreaction level and can protect animals from the attack of aftosa epidemic strains. The Asia I-type aftosa synthetic peptide vaccine prepared by the polypeptide antigens can effectively cope with the antigen variation of aftosa virus without causing biosafety problems and has easy large-scale synthesis, thereby having a good application prospect.

The invention provides an Asia I synthetic peptide vaccine of a foot and mouth disease, in particular to a polypeptide or a polypeptide polymer thereof for the Asia I synthetic peptide vaccine of thefoot and mouth disease, the vaccine containing the polypeptide or the polypeptide polymer thereof, and a preparation method of both, wherein, the amino acid sequence of the polypeptide is shown as SEQID NO:1. In the invention, variation situation of main antigen sites of the foot and mouth disease is researched by determining a sequence of an epidemic strain of the foot and mouth disease, and meanwhile analysis prediction of the antigen sites of the foot and mouth disease is performed followed by chemical synthesis on possible peptide segments of the antigen sites combined with a computer auxiliary method; and candidate polypeptide antigens are screened through a large quantity of animal experiments to finally obtain the polypeptide antigens with high immune reaction level which can protect well an animal from being attacked by the epidemic strain of the foot and mouth disease. The vaccine prepared from the polypeptide antigens has the advantages of no biological safety problem, easylarge-scale synthesis, good application prospect and good ability of effectively dealing with antigen variation of foot and mouth disease virus.

The invention discloses an Escherichia coli strain reformed by gene engineering, and applications thereof. According to the present invention, engineering bacteria for preventing and controlling smutis constructed by using the applications of methyl jasmonate in prevention and controlling of smut, and recipient bacteria over-express methyl jasmonate transferase; the metabolite of the engineeringbacteria is methyl jasmonate, such that the biosafety problem does not exist; jasmonate methyltransferase is derived from plants, and cannot cause biosafety problem after being expressed by Escherichia coli; the engineering bacteria are Escherichia coli, and has characteristics of simple culture conditions, low cost and simple implementation method; the method for improving the defense ability byincreasing the content of the hormone methyl jasmonate in the plant does not affect the growth of the plant; and with the application of the engineering bacteria in prevention and controlling of CornLump Smut, the significant prevention and control effect can be achieved, and the Escherichia coli strain reformed by gene engineering is worthy of being popularized and applied.

The invention discloses a new antigen display system based on insect viral capsid and a construction method of the new antigen display system. The steps of the system construction method are as follows: (1) determining an insertion site of a heterologous protein; (2) successfully constructing a library of WhNV recombinant viroid particle VLP fused with enhanced green fluorescent protein EGFP; (3)constructing a library of Wuhan Noda Village recombinant virus particles fused with H5 antigen protein of human avian influenza virus; and (4) establishing a virus display system of Wuhan Noda Village. The system comprises: (1) WhNV capsid of Wuhan Noda Village virus, (2) a fusion protein as the heterologous protein inserted into the WhNV capsid protein, and (3) linkers, small amino acid sequencesrespectively added to the N-terminal and C-terminal of the fusion protein as linkers to connect with the WhNV capsid. According to the present invention, the construction method is simple and easy tooperate, and the WhNV heterologous antigen display system successfully established by the method lays a good foundation for the establishment of an efficient, stable and highly versatile platform forthe development of influenza VLP vaccine, and then for the establishment of platforms for the development of multiple vaccines and drugs.

The invention relates to a specific promoter in a leguminous plant root system tissue and application thereof. The promoter is particularly suitable for driving the specific expression of exogenous genes in the leguminous plant root tissue, and the expression level of the exogenous genes in other tissues of leguminous plants is extremely low or the exogenous genes are not expressed in the other tissues of the leguminous plants. The expression specificity and specificity of the promoter are very high. The invention further provides constructions, expression kits, carriers and the like with the promoter. For transgenic leguminous plants containing the promoter disclosed by the invention, agronomic traits of the leguminous plants can be specifically improved, so that the influence on the other tissues of the leguminous plants caused by the introduction of the exogenous genes is effectively avoided, the exogenous genes can be specifically expressed in the root tissue, and thus, various related products are obtained.