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Co-expression vector of protective antigens momp and mip of Chlamydia abortus and Chlamydia psittaci and its construction and expression method

A technology of co-expression vectors and expression vectors, applied in the direction of Chlamydia antigen components, chemical instruments and methods, and the use of vectors to introduce foreign genetic materials, etc., can solve problems such as inactivation, economic loss in animal husbandry, vaccine recombination, etc., and achieve quality control , Overcome the effect of insufficient expression of tandem expression

Active Publication Date: 2017-02-22
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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Problems solved by technology

The mixed infection of these two kinds of chlamydia has been found in many animal abortions and other cases. Therefore, the mixed infection of abortive chlamydia and psittaci-tropic chlamydia has caused huge economic losses to the animal husbandry
[0003] In response to the above problems, the field has tried to develop new vaccines such as live vector vaccines, nucleic acid vaccines, gene deletion vaccines, etc., but these vaccines have the possibility of recombination and inactivation in nature

Method used

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  • Co-expression vector of protective antigens momp and mip of Chlamydia abortus and Chlamydia psittaci and its construction and expression method
  • Co-expression vector of protective antigens momp and mip of Chlamydia abortus and Chlamydia psittaci and its construction and expression method
  • Co-expression vector of protective antigens momp and mip of Chlamydia abortus and Chlamydia psittaci and its construction and expression method

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Embodiment Construction

[0042] In the following examples, the present invention provides a specific implementation path of the technical solution, and provides various types of reagents that are specifically used. However, the present invention is not limited to other experimental methods, as long as the present invention can be obtained. The co-expression vector is the protection scope of the present invention.

[0043] In the following examples, the main materials used include:

[0044] Chlamydia abortus strain, Chlamydia psittaci strain, available from strain companies. For the convenience of labeling in the experiment, they were named SX5 and D13 respectively.

[0045] The dual expression vectors of pETDuet-1 and pRSFDuet-1, pET-32a(+) vector were purchased from Novagen.

[0046] Expression bacteria: BL21(DE3) is the expression host bacteria of the four toxin proteins, purchased from Beijing Quanshijin Biotechnology Co., Ltd.

[0047] Primers were synthesized by Shanghai Sangon Bioengineering ...

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Abstract

The invention discloses a coexpression vector of Chlamydophila abortus and Chlamydophila psittaci protective antigen MOMP and MIP, a construction method and an expression method thereof. By means of a molecular cloning technique, the coding genes of Chlamydophila abortus's main outer membrane protein (MOMPa) and macrophage infection potentiator (MIPa) and Chlamydophila psittaci's main outer membrane protein (MOMPp) and macrophage infectivity potentiator (MIPp) are respectively connected to the dual expression vector pETDuet-1 and pRSFDuet-1 so as to construct the coexpression vector. The coexpression vector provided by the invention carries four protein genes of MOMP and MIP of Chlamydophila abortus and Chlamydophila psittaci, can realize expression in one host bacterium, and can be used for preparing polyvalent chlamydial genetic engineering subunit vaccines and preventing and controlling multi-serotype animal infectious diseases, is in favor of improving the technological level for controlling animal chlamydial diseases in China, thus ensuring the sound development of livestock raising industry, increasing the income of farmers and herdsmen, and guaranteeing public health and animal product safety.

Description

technical field [0001] The invention relates to a construction and expression method of a chlamydia protective antigen MOMP and MIP co-expression vector, belonging to the field of biology. Background technique [0002] Chlamydia is a Gram-negative obligate intracellular parasitic prokaryote that can infect humans and animals and cause a variety of diseases. Chlamydophila abortus (Chlamydophila abortus) and Chlamydophila psittaci (Chlamydophila psittaci) are two common pathogens of animal diseases. Due to the similarity in structure and pathogenic mechanism, both of them can infect cattle, sheep, goats and pigs, etc. species with the same host range. Among them, Chlamydia aborophilus mainly parasitizes the placental trophoblast cells of animals, causing placental inflammation, resulting in abortion, premature birth, stillbirth, mummified fetus or weak fetus in pregnant dams, and can also cause abortion, pneumonia, enteritis, and polyarthritis in infected animals and other s...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/70C12N15/66C07K14/295A61K39/118A61P31/00
Inventor 周继章李兆才曹小安宫晓炜陈启伟
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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