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Huoxiang Zhengqi liquid quality control method

A quality control method, the technology of Huoxiangzhengqi water, which is applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of complex chemical composition and unclear medicinal effect, and achieve high repeatability and ensure stable and consistent effects.

Inactive Publication Date: 2012-07-04
GUANGDONG PHARMA UNIV
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Problems solved by technology

[0004] Huoxiang Zhengqi Water is composed of ten traditional Chinese medicines, and its chemical composition is relatively complex
The 2010 edition of the Chinese Pharmacopoeia only measures the content of four components, honokiol, magnolol, liquiritin, and hesperidin. However, the efficacy of the compound Chinese medicine is the result of the joint action of multiple components, and the controlled components The pharmacological effect is not clear

Method used

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  • Huoxiang Zhengqi liquid quality control method
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  • Huoxiang Zhengqi liquid quality control method

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Embodiment Construction

[0035] The present invention will be further described below in conjunction with specific embodiments, but it is not limited thereto.

[0036] A kind of quality control method of Huoxiang Zhengqi water, comprises the steps:

[0037]1) Preparation of the solution of the product to be tested: Take 300 μL of the product to be tested in Huoxiang Zhengqi Water, dilute to 1 mL with water, extract 3 times with ethyl acetate, take 2 mL of ethyl acetate each time, combine the extracts, and evaporate the acetic acid to dryness Ethyl ester, dilute to 10 mL with methanol, shake well, filter, and obtain;

[0038] 2) The product solution to be tested is carried out by high-performance liquid chromatography under the following chromatographic conditions to obtain the high polarity part fingerprint of the product to be tested:

[0039] Chromatographic column adopts Diamonsil C 18 ; Using gradient elution, mobile phase A is 0.05% formic acid aqueous solution, B is acetonitrile, C is methanol...

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Abstract

The invention discloses a Huoxiang Zhengqi liquid quality control method, which comprises the following steps of: preparing solution of products to be tested; testing a fingerprint map of solution of the products to be tested; and carrying out comparison with the set high-polarity part standard fingerprint map using liquiritin as reference objects and the medium and low-polarity part standard fingerprint map using nobiletin as reference objects for judging whether the products to be tested are qualified or not. According to the method, the major effective ingredients in the Huoxiang Zhengqi are used as the basis, a reasonable quality control method is built, and the product quality can be effectively controlled. The quality control method has the advantages that the precision, the stability and the repeatability are higher, and the stability and the uniformity of the product quality are ensured, so the safety and the effectiveness of the product are ensured.

Description

technical field [0001] The invention relates to a quality control method for Chinese patent medicines, in particular to a method for controlling the quality of Huoxiangzhengqi water by using high-performance liquid chromatography (HPLC) fingerprints. Background technique [0002] Huoxiang Zhengqi Water is a commonly used medicine for relieving heat in summer. It is mainly composed of Huoxiang, Atractylodes atractylodes, Chenpi, Magnolia officinalis, Angelica dahurica, Poria, Dabupi, pinellia, licorice, perilla and other traditional Chinese medicines. Spasm, enhance cellular immune function and antibacterial effect. [0003] Traditional Chinese medicine fingerprint refers to the chromatogram that can mark the chemical characteristics of some Chinese medicinal materials or preparations after proper treatment and certain analysis methods. Fingerprint of traditional Chinese medicine is a comprehensive and quantifiable means of identification. It is based on the systematic study...

Claims

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Application Information

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IPC IPC(8): G01N30/88
Inventor 李康吴韶辉张洪坤黄洋
Owner GUANGDONG PHARMA UNIV
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